This article presents the results of a study of energy and ecological indicators at different engine loads (BMEP) adjusting the Start of Injection (SOI) of a Compression Ignition Engine fuelled with blends of diesel (D), rapeseed methyl ester (RME)-based biodiesel and isopropanol (P). Fuel blends mixed at D50RME45P5, D50RME40P10 and D50RME30P20 proportions were used. Alcohol-based fuels, such as isopropanol, were chosen because they can be made from different biomass-based feedstocks and used as additives with diesel fuel in diesel engines. Diesel fuel and its blend with 10% alcohol have almost the same thermal efficiency (BTE). In further examination of energy and ecological indicators, combustion parameters were analysed at SOI 6 CAD BTDC using AVL BOOST software (BURN subprogram). Increasing alcohol content in fuel blends led to a reduced cetane number, which prolonged the ignition delay phase and intensified heat release in the premixed combustion phase. Higher combustion temperatures and oxygen content in the fuel blends increased NOx emissions. Lower C/H ratios and higher O2 levels affected by RME and isopropanol reduced smoke emissions.