The purpose of this research is to analyze the role of organizational culture in fostering green practices in the workplace while investigating the mediating role of intrinsic motivation in the context of energy conservation. Based on a cross-sectional quantitative study with a sample of 203 employees from the manufacturing sector, the hypothesized relationships were verified. Based on the mediation analysis, statistical analyses revealed positive relationships between organizational culture and green workplace practices, as well as organizational culture and intrinsic motivation. Additionally, the study found that intrinsic motivation mediates the relationship between organizational culture and green workplace practices. This study supported the importance of organizational culture in enhancing green workplace practices aimed at conserving energy and natural resources. The underlying mechanism behind the significant positive effect of intrinsic motivation on proenvironmental behavior in the workplace was also identified. The research demonstrates the importance of an organizational culture to reinforce green practices in the workplace. Furthermore, based on the used models, this study illustrates the importance of each organizational culture dimension: leadership, sense of community, communication, collaboration, and structure in promoting green behavior. Additionally, the research suggests a key role of intrinsic motivation in this relationship. This study provides valuable guidance for the implementation of specific environmental measures in companies.