Currently, studies regarding the factors influencing the real-world electricity consumption of electric motorcycles are lacking. The objective of this study was to examine the factors influencing the real-world electricity consumption of electric motorcycles when driving along an uncongested road network. This study developed an onboard measurement device to collect on-road data, including instant speed data and electricity consumption, from the test electric motorcycle while it was driving on a real-world road. Overall, 105 participants (n = 105) drove the test motorcycle along the uncongested urban road network. Multiple linear regression analysis was applied to explore the effect of influencing variables on the electricity consumption of electric motorcycles. The analysis results revealed that the rider’s weight and average running speed positively influenced electricity consumption, whereas decelerating time negatively influenced electricity consumption. Noticeably, the rider’s weight affected electricity consumption more than other factors. The lightweighting of electric motorcycles was mainly recommended to lower electricity consumption. Subsequently, CO2 emissions from electricity generation could be reduced.