Increasing the feed total solids to anaerobic digester improves the process economics and decreases the volume of liquid effluent from current wet anaerobic digestion. The objective of this study was to develop a novel psychrophilic (20 °C) anaerobic digestion technology of undiluted cow feces (total solids of 11%–16%). Two sets of duplicate laboratory-scale sequence batch bioreactors have been operated at organic loading rates (OLR) of 6.0 to 8.0 g total chemical oxygen demand (TCOD) kg−1 inoculum day−1 (d−1) during 210 days. The results demonstrated that the process is feasible at treatment cycle length (TCL) of 21 days; however, the quality of cow feces rather than the OLR had a direct influence on the specific methane yield (SMY). The SMY ranged between 124.5 ± 1.4 and 227.9 ± 4.8 normalized liter (NL) CH4 kg−1 volatile solids (VS) fed d−1. Substrate-to-inoculum mass ratio (SIR) was 0.63 ± 0.05, 0.90 ± 0.09, and 1.06 ± 0.07 at OLR of 6.0, 7.0, and 8.0 g TCOD kg−1 inoculum d−1, respectively. No volatile fatty acids (VFAs) accumulation has been observed which indicated that hydrolysis was the rate limiting step and VFAs have been consumed immediately. Bioreactors performance consistency in terms of the level of SMYs, VFAs concentrations at end of the TCL, pH stability and volatile solids reduction indicates a stable and reproducible process during the entire operation.