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Universal Capacitance Model for Real-Time Biomass in Cell Culture

Capacitance probes have the potential to revolutionize bioprocess control due to their safe and robust use and ability to detect even the smallest capacitors in the form of biological cells. Several techniques have evolved to model biomass statistically, however, there are problems with model transfer between cell lines and process conditions. Errors of transferred models in the declining phase of the culture range for linear models around +100% or worse, causing unnecessary delays with test runs during bioprocess development. The goal of this work was to develop one single universal model which can be adapted by considering a potentially mechanistic factor to estimate biomass in yet untested clones and scales. The novelty of this work is a methodology to select sensitive frequencies to build a statistical model which can be shared among fermentations with an error between 9% and 38% (mean error around 20%) for the whole process, including the declining phase. A simple linear factor was found to be responsible for the transferability of biomass models between cell lines, indicating a link to their phenotype or physiology.
- TU Wien Austria
- Boehringer Ingelheim Fonds Germany
- Boehringer Ingelheim Fonds Germany
- TU Wien Austria
Models, Statistical, Chemical technology, capacitance, statistical model, Cell Culture Techniques, PLS, TP1-1185, CHO Cells, Electric Capacitance, Models, Biological, Article, Cricetulus, Cricetinae, Animals, Biomass, fed batch, CHO cell culture
Models, Statistical, Chemical technology, capacitance, statistical model, Cell Culture Techniques, PLS, TP1-1185, CHO Cells, Electric Capacitance, Models, Biological, Article, Cricetulus, Cricetinae, Animals, Biomass, fed batch, CHO cell culture
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).31 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
