This work calculates and discusses the Life Cycle Assessment (LCA) of four scenarios composed of two types of road pavements and two types of lighting systems to be built in an Italian twin-tube road tunnel. A 20-year time horizon is adopted to assess the burdens of construction and maintenance of both flexible and rigid pavements and high-pressure sodium (HPS) and light-emitting diode (LED) lamps, traffic, and switching on of lamps. All considered scenarios are comparable with each other in terms of technical performances, but significantly differ regarding their environmental consequences. The geometrical and technical characteristics of the examined scenarios comply with current Italian standards for highways. In all the examined cases, LCA is carried out according to the European standard, EN 15804, and includes 19 impact categories (IC). The analysis demonstrates that the use of more reflecting surface pavement materials (i.e., concrete vs. asphalt) and more performing lighting systems (i.e., LED vs. HPS) can effectively mitigate the deleterious burdens related to road construction, maintenance, and use. For most of the examined ICs, the most environment-friendly scenario has LED lamps and concrete pavement.