Response scales in auditory perception assessment are critical for capturing the true responses of listeners. Despite its impact on data, response scales have received the least attention in auditory perception assessment. In this study, the usability of visual analogue scales for auditory perception assessment was investigated. Five response scales (a unipolar visual analogue scale–negated to regular, a unipolar visual analogue scale—regular to negated, a bipolar visual analogue scale–positive to negative, a bipolar visual analogue scale—negative to positive, and a unipolar 11-point scale (ISO/TS 15666:2021)) for auditory perception assessment are presented. Music and traffic noise were presented to 60 university students at two different levels, i.e., 45 and 65 dBA, respectively. A web-based experimental design was implemented, and tablet pads were provided to the respondents to record their responses. The unipolar 11-point scale required the longest response time, followed by the two unipolar visual analogue scales and two bipolar visual analogue scales with statistical significance. All response scales used in this study achieved statistical reliability and sensitivity for the auditory perception assessment. Among the five response scales, the bipolar visual analogue scale (negative to positive) ranked first in reliability over repeated measures, exhibited sensitivity in differentiating sound sources, and was preferred by the respondents under the conditions of the present study. None of the respondents preferred the unipolar 11-point scale. The visual analogue scale was favoured over the traditional unipolar 11-point scale by young educated adults in a mobile-based testing environment. Moreover, the bipolar visual analogue scale demonstrated the highest reliability and sensitivity, and it was preferred the most by the respondents. The semantic labelling direction from negated to regular, or from negative to positive, is preferred over its opposite counterpart. Further research is necessary to investigate the use of response scales for the general public including children and the elderly, as well as that of semantic adjectives and their counterparts for auditory perception assessment.