The objective of the study was the translation and adaptation into Arabic language of the Questionnaire on Teacher Interaction and the examination of psychometric properties among students in Tunisia. A set of students (N = 778) were recruited to complete a translated, Arabic version of the Teacher Interaction Questionnaire (AQTI). The subjects represented both female and male with an average age of 15.98 ± 2.17 years. The subjects were divided into exploratory (N = 226) and confirmatory (N = 552) samples. For the exploratory sample, the female sex represented 46.90% and the male 53.10% (N = 120). Likewise, the confirmatory sample consisted of females (48.01%) and males (51.99%). The scale designed in Arabic AQTI presented eight components for exploratory factor analysis. The result was a first factorial solution, which kept these eight components of the predetermined instrument. The confirmatory factor analysis provided good fit indices. Similar results for instrument reliability were confirmed for all dimensions, through good Cronbach’s coefficient, which were superior to 0.80. The Arabic version of AQTI is valid and reliable and can be administered to assess teacher/student interactions in Tunisia. However, further research needs to examine the construct validity of the instrument.