Academic stress and burnout are the predominant factors that can negatively affect student performance and sustainable learning. Therefore, it is important to analyze the factors related to student academic burnout in preservice teachers in western China. 212 respondents from public universities in Guangxi Province participated, and the data were analyzed using partial least-squares structural equation modeling (PLS-SEM) to check reliability, validity, and initial hypothesis testing. The results show that perfectionism, excessive self-efficacy, and workload are the main factors causing academic stress and burnout in preservice teachers. These problems can be reduced by increasing self-efficacy and coping strategies of preservice teachers. In addition, this study provides important knowledge to universities based on factors related to preservice teachers’ academic stress and burnout, as well as strategies and solutions to reduce these problems in students.