Recreational hunting can have positive consequences on biodiversity conservation, but in many countries the number of hunters is declining. This downward trend threatens the sustainability of an important human activity that can be used as a tool for wildlife management and biodiversity conservation. On the other hand, in developed countries there is an upward trend in the number of female hunters. In this study, we analyzed women and men’s hunting preferences in Spain, focusing our attention on the game species they were interested in. We found that female hunters were more interested than male hunters in hunting big game species. We discussed potential consequences of our results on the sustainability of hunting and biodiversity conservation.