This article focuses on the role that the provisions of the Natura 2000 Network play in affecting land-taking processes by looking at the Italian region of Sardinia, where strict rules on land development have been enforced since 1993 through regional landscape plans and where an extensive Natura 2000 Network, covering nearly 19% of the regional land mass, was established in compliance with Directive 92/43/EEC on the conservation of natural habitats and of wild fauna and flora and Directive 2009/147/EC on the conservation of wild birds. The results and inferences of our study could be easily generalized to other European Union regions, provided that similar geographic datasets are available. By shedding some light on the relation between land take on the one hand, and nature conservation and landscape protection on the other, it is possible to enhance regional planning policies to prevent or hinder land-taking processes, and, by doing so, to help implementing the European Commission recommendation on no net land take by 2050 into the EU regional policies.