ABSTRACT: Ryegrass seeding may be limited by the lack of farming machinery. The aim of this study was to evaluate the establishment of three ryegrass (Lolium sp.) genotypes (annual diploid, annual tetraploid, and hybrid tetraploid) in kikuyu grass pastures (Pennisetum clandestinum) with seeding based on zero tillage. The first agronomic evaluation of ryegrass was conducted 60 days after seeding and then every 35 days until the fourth grazing. For data analysis we used a linear mixed model with grass genotype, topography, and number of cuts as fixed effects. The random effect was the farm. Biomass and establishment percentage were better in the high area (flat terrain) for the three genotypes (p<0.05). Biomass of annual diploid ryegrass was higher in the high and slope area (p<0.05). The leaf/stem ratio, height, length, and width of the last and fully elongated leaf were statistically significant (p<0.05) between the different cuts for all genotypes, showing that grass growth increases with the number of cuts when rational grazing management is provided. The results of this study support the conclusion that greater adaptation of diploid ryegrass associated with Kikuyu grass positively impacts biomass production under zero tillage. ; RESUMEN: La siembra de ryegrass puede estar limitada por la carencia de maquinaria en las explotaciones ganaderas. El objetivo de este estudio fue evaluar el establecimiento de tres genotipos de ryegrass (Lolium sp.) -anual diploide, anual tetraploide, e híbrido tetraploide- en pasturas de kikuyo (Pennisetum clandestinum) con siembra basada en cero labranza. La primera evaluación agronómica del ryegrass se hizo 60 dias luego de la siembra y luego cada 35 días, hasta el cuarto pastoreo. Para el análisis de la información se empleó un modelo lineal mixto con genotipo del forraje, topografía, y número de corte como efectos fijos. El efecto aleatorio fue finca. La biomasa y el porcentaje de establecimiento fueron mejores en la zona alta (terreno plano) (p<0,05) del potrero en ...