A batch-activated sludge process (AS) has been applied to eliminate sodium dodecylbenzenesulfonate (NaDBS), a linear alkylbenzenesulfonate (LAS) compound, from synthetic and real domestic wastewater. The kinetics of surfactant biodegradation was best described by first-order kinetics with rate constants of 1.28 and 1.15 L·(g VSS·day)-1 for synthetic and real domestic wastewater, respectively. The effect of preozonation on the overall surfactant and COD removal rates was also studied. The results indicated that treatment by combined ozone−AS leads to negligible foam ability and low residual surfactant concentration and COD in the treated effluent. Acclimation of a mixed culture to ozonated products was beneficial to highly improve biodegradation rates after preozonation. Thus, when applying ozone at a dose of 100 mg·L-1, the first-order surfactant biodegradation rate constant increased up to 1.79 and 3.09 L·(g VSS·day)-1 for synthetic and real wastewater, respectively. Continuous experiments of ozonation f...