One of the four major crops worldwide is the potato. Despite the production of potatoes in Korea, most of them are being imported from other countries. The 15 viral diseases are monitored and tested whenever potatoes are imported in Korea. In this study, there are 2 sets of RT-PCR primer for diagnosing the Andean potato mottle virus (APMoV), which is a type of monitored quarantine viruses infecting potatoes, wherein each nested PCR and modified-positive control plasmid that can verify laboratory contamination and false positive response were developed. The RT-PCR primer sets for analyzing the APMoV were 315 and 732 bp amplified, and each nested PCR were 128 and 391 bp amplified. In addition, the modified-positive control plasmid, which can be used for the PCR diagnosis system, was also developed in order to realize a precision test system for fast and accurate diagnoses of APMoV in imported potatoes. The APMoV PCR diagnosis system, which has been developed in this study, can be used for a possible quarantine in the future. It can also contribute to the increase in the potato production worldwide.