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Acta Crystallographica Section F Structural Biology Communications
Article . 2014 . Peer-reviewed
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Crystallization and preliminary X-ray crystallographic analysis of a putative feruloyl esterase fromTalaromyces cellulolyticus

Authors: Masahiro Watanabe; Kazuhiko Ishikawa;

Crystallization and preliminary X-ray crystallographic analysis of a putative feruloyl esterase fromTalaromyces cellulolyticus

Abstract

Feruloyl esterase (FAE; EC 3.1.1.73) catalyzes the cleavage of the ester bond between ferulic acid and polysaccharides in plant cell walls, and thus holds significant potential for the industrial utilization of biomass saccharification. A feruloyl esterase was identified from the genome database ofTalaromyces cellulolyticus(formerly known asAcremonium cellulolyticus). The gene consists of the catalytic domain and a carbohydrate-binding module connected through a serine/threonine-rich linker region. The recombinant enzyme was prepared, purified and crystallized at 293 K using 0.1 Mimidazole pH 8.0, 0.2 Mcalcium acetate, 14% PEG 8000 as the precipitant. The crystal diffracted to 2.6 Å resolution and the crystal system is primitive orthorhombic, with unit-cell parametersa= 90.9,b= 123.4,c= 135.4 Å. Four molecules are assumed to be present per asymmetric unit, corresponding to a Matthews coefficient of 2.50 Å3 Da−1and a solvent content of 50.88%(v/v).

Keywords

Talaromyces, Crystallization, Crystallography, X-Ray, Carboxylic Ester Hydrolases

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Average
Average
Average
bronze