Additional file 1: of No involvement of alveolar macrophages in the initiation of carbon nanoparticle induced acute lung inflammation in mice
Shanze Chen; Renfu Yin; Mutze, Kathrin; Youjia Yu; Takenaka, Shinji; Kรถnigshoff, Melanie; Stoeger, Tobias;
Additional file 1: of No involvement of alveolar macrophages in the initiation of carbon nanoparticle induced acute lung inflammation in mice
Additional Methods. Figure S1. Ultrastructure of CNP-aerosols (A) and aqueous dispersion (B). Figure S2. Enrichment of cell-specific gene expression in CD45- and CD45+ lung cells and total BAL cells. Figure S3. Purity of isolated primary murine ATII cells. Figure S4. Synopsis of gene expression results of all four cell isolation experiments. Figure S5A. Gene expression profile of by BAL macrophages 3 and 12 h after CNP and 12 h after LPS instillation. Figure S5B. BAL neutrophil and macrophage numbers 3 and 12 h after CNP and 12 h after LPS instillation. Figure S6. Detection of inflammatory cell activation by immunostaining, we used NFkB-GFP-reporter mice. Additional Reference. (PDF 541 kb)