This work focuses on cellular toxicity of PET-based hollow fiber containing silver particles (Ag/PET hollow fiber), which was synthesized by different pressure method. Hela cells were used to investigate in vitro cytotoxicity of Ag/PET hollow fiber with the method of Methyl Thiazolyl Tetrazolium (MTT) assay. And the results showed no significant toxicity to Hela cells. And the SEM images of Hela cells treated by Ag/PET hollow fibers showed that cells morphology was unaltered in the present of Ag/PET hollow fiber. However, abnormal size, shrinkage and rounded appearance of cells at higher dose (20mg/ml)suggested slight toxicity of Ag/PET hollow fiber. Combining the antibacterial concentration (37.5μg/ml) in the pre-works, it was found that there was a certain concentration of silver ions which can achieve a minimization of cytotoxicity and a maximization of antibacterial efficacy.