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The objective of this study was to determine effects of drought on selected root growth parameters and develop relationships between root parameters and tuber yield for selected Jerusalem artichoke (JA) genotypes. Three water regimes (Field capacity, 50% available soil water (AW) and 25% AW) and five JA varieties (JA 60, JA 125, JA 5, JA 89 and HEL 65) were planted with factorial treatments in a randomized complete block design with four replications. Data on root dry weight (RDW) and root: shoot ratios (RSR) were measured manually. Root diameter (RD), root length (RL), root surface area (RSA) and root volume (RV) were collected at harvest. Drought tolerance indices (DTI) were calculated for all root parameters. Drought reduced all root parameters and DTI but increased RSR in JA 60, JA 125, JA 5, and HEL 65. JA 125 had high values for all root traits and DTI of these traits under drought stress. JA 60 had high DTI of RDW, RD and RSR under mild and severe water stress. JA 5 had high DTI of RDW, RD, RL, RSR and RV under drought conditions. JA 89 and HEL 65 performed well for RDW, RD, RL and low DTI of all root characteristics. DTI for root parameters were positively correlated with tuber dry weight under mild and severe water stress. The JA 5, JA 60 and JA 125 varieties showed high DTI for some root traits, indicating that better root parameters contributed to higher tuber yield under drought stress.

Abstract Limited information is available regarding biomass production potential of long-term (>5- yr-old) switchgrass (Panicum virgatum L.) stands. Variables of interest in biomass production systems include cultivar selection, site/environment effects, and the impacts of fertility and harvest management on productivity and stand life. We studied biomass production of two upland and two lowland cultivars under two different managements at eight sites in the upper southeastern USA during 1999–2001. (Sites had been planted in 1992 and continuously managed for biomass production.) Switchgrass plots under lower-input management received 50 kg N ha−1 yr−1 and were harvested once, at the end of the season. Plots under higher-input management received 100 kg N ha−1 (in two applications) and were harvested twice, in midsummer and at the end of the season. Management effects on yield, N removal, and stand density were evaluated. Annual biomass production across years, sites, cultivars, and managements averaged 14.2 Mg ha−1. Across years and sites, a large (28%) yield response to increased inputs was observed for upland cultivars; but the potential value of higher-input management for lowland cultivars was masked overall by large site×management interactions. Nitrogen removal was greater under the higher-input system largely due to greater N concentrations in the midsummer harvests. Management recommendations (cultivar, fertilization, and harvest frequency), ideally, should be site and cultivar dependent, given the variable responses reported here.

Abstract In this paper we analyze the economic impact of a decision to produce ethanol in Mexico, comparing the effect of a subsidy to initiate ethanol production with that of alternative public policies. Public support of biofuels has been a public policy goal since 2008, and the promotion of ethanol remains an active part of the government agenda. The evidence used to encourage or alter the policy is (by necessity) chiefly based on international experience. In this study we use a computable general equilibrium model (CGE) to estimate the impact of ethanol production on the Mexican economy. Using cost data from Brazil we introduce ethanol into a Mexican social accounting matrix, and insert a latent sector into the model to analyze ethanol promotion. Our results show that subsidies to ethanol would increase agriculture production but at the expense of aggregate welfare. By contrast, alternative "clean energy" policies appear to advance economic growth to a greater extent.

We investigate the spatiotemporal patterns and environmental controls of the end of the vegetation growing season (EOS) in autumn across the alpine and temperate grasslands of China from 2001 through 2020, focusing on whether the EOS is likely a "dryness effect" due to drought or a "coolness effect" caused by cold temperature in autumn. The results show that the EOS date is earlier (∼6 days earlier on average) in alpine grasslands than in temperate grasslands. During 2001-2020, a slight non-significant delay of 1.0 day/decade is observed for the regional averaged EOS, which is mostly induced by the delayed EOS in 64.4 % of the study region. Preseason temperature (1-2 months before the EOS) exerts a positive control on the EOS in most of the alpine grasslands and some regions of the eastern part of the temperate grasslands, while drought with a mean length of 3.2 months before the EOS exerts positive effects on the EOS in the central, southwestern, and western parts of the temperate grasslands and in the northeastern part of the alpine grasslands. The positive effects of temperature and drought are very likely phenomena reflecting that the EOS is the "coolness effect" associated with lower temperatures in autumn and the "dryness effect" due to drought, especially meteorological drought without consideration of soil moisture, in late summer and/or early autumn, respectively. Our findings are supported by an analysis of the spatial patterns of the cold degree days (CDD) and EOS sensitivity to the CDD. However, the negative effects of drought are also found in eastern temperate grasslands, likely caused by decreased temperature accompanied by increased moisture. The results presented here highlight the importance of incorporating the impacts of droughts on EOS variability, as well as their interactive effects with temperature, into current vegetation autumn phenology models for grasslands.

Ethanol elimination rates were determined in rats using an intravenous route of ethanol administration after several experimental manipulations. Twenty-four hr food deprivation resulted in a 30% reduction to 35 mg/100ml blood/hr in elimination rate from a non-deprived rate of 50 mg/100 ml blood/hr. After 2 months of ethanol drinking (5% v/v), 24 hr starvation resulted in only a 10% reduction in elimination rate (45 mg/100 ml blood/hr), and did not increase the non-food-deprived rate (49.2 mg/100 ml blood/hr) over that obtained in the above animals' drinking water rather than 5% ethanol. Animals which chronically overdrank ethanol or water for 3 months on a schedule-induced polydipsia procedure, known to result in ethanol physical dependence, showed a decreased rate of ethanol elimination (37.9 mg/100 ml blood/hr for water drinkers) in the non-food-deprived condition. By providing 750 mg of liver powder daily as a food supplement in the ethanol overdrinking regimen, the ethanol elimination rate remained at a rate comparable to the normal animal (48.4 mg/100 ml blood/hr).

AbstractHigher temperatures caused by future climate change will bring more frequent heat stress events and pose an increasing risk to global wheat production. Crop models have been widely used to simulate future crop productivity but are rarely tested with observed heat stress experimental datasets. Four wheat models (DSSAT‐CERES‐Wheat,DSSAT‐Nwheat,APSIM‐Wheat, and WheatGrow) were evaluated with 4 years of environment‐controlled phytotron experimental datasets with two wheat cultivars under heat stress at anthesis and grain filling stages. Heat stress at anthesis reduced observed grain numbers per unit area and individual grain size, while heat stress during grain filling mainly decreased the size of the individual grains. The observed impact of heat stress on grain filling duration, total aboveground biomass, grain yield, and grain protein concentration (GPC) varied depending on cultivar and accumulated heat stress. For every unit increase of heat degree days (HDD, degree days over 30 °C), grain filling duration was reduced by 0.30–0.60%, total aboveground biomass was reduced by 0.37–0.43%, and grain yield was reduced by 1.0–1.6%, butGPCwas increased by 0.50% for cv Yangmai16 and 0.80% for cv Xumai30. The tested crop simulation models could reproduce some of the observed reductions in grain filling duration, final total aboveground biomass, and grain yield, as well as the observed increase inGPCdue to heat stress. Most of the crop models tended to reproduce heat stress impacts better during grain filling than at anthesis. Some of the tested models require improvements in the response to heat stress during grain filling, but all models need improvements in simulating heat stress effects on grain set during anthesis. The observed significant genetic variability in the response of wheat to heat stress needs to be considered through cultivar parameters in future simulation studies.

Maternal ethanol (ETOH) exposure is associated with impaired fetal growth. Because insulin‐like growth factors (IGFs) are thought to be important in the regulation of fetal somatic growth, we examined the influence of maternal ETOH exposure on fetal growth and plasma levels of IGF‐I, IGF‐II, and IGF binding proteins (IGFBPs) in the rat model. Control (A) dams were fed a standard rat chow ad libitum. ETOH (E) consuming dams were fed a 36% ETOH diet, and pair‐fed (P) dams were fed isocaloric amounts of a control liquid diet. All animals were killed on day 20 of gestation. Plasma concentrations of IGF‐I and ‐II were determined by radioimmunoassay after formic acid‐acetone extraction and heat inactivation of IGFBPs. Levels of IGFBPs in fetal plasma were estimated by Western ligand blotting after protein separation by SDS‐PAGE and electrotransfer to nitrocellulose. Membranes were probed with [125l]IGF‐l, and IGFBPs were identified by autoradiography, quantified by scanning densitometry and results expressed relative to corresponding IGFBPs in control fetal plasma.Maternal weight gain from conception to 20 days of pregnancy was reduced for E compared to P and A dams (p < 0.05 E vs. P or A). The same pattern was reflected in fetal weight that tended to be lower in P compared with A pups, and was significantly reduced in E pups compared with both groups (p < 0.0001 E vs. P or A). Thus, fetal growth was more retarded in E animals despite equal caloric and protein intake by E and P dams.Radioimmunoassay of fetal plasma revealed that there was no difference in circulating levels of IGF‐II in A (65.5 ± 8.7 ng/ml) and P (65.9 ± 9.8 ng/ml) plasma. However, levels of IGF‐II were higher in E (87.1 ± 6.2 ng/ml) than in corresponding P animals. At the same time, there were no differences in IGF‐I levels between any of the treatment groups. Western ligand blotting demonstrated that levels of 32–34 kDa IGFBPs were elevated in plasma of P compared with A fetal plasma, consistent with an effect of reduced maternal nutrition. In contrast, levels of32–34 kDa IGFBPs were reduced in E plasma compared with P and A (p < 0.04 and p < 0.02, respectively).These data suggest that circulating levels of IGF‐II (but not IGF‐I) and specific IGFBPs are altered in growth‐retarded fetuses exposed to ETOH. Comparison to fetal plasma obtained from pair‐fed litters demonstrate that these effects on IGF and IGFBP levels are specific to ETOH, and not simply due to maternal nutrient intake. Taken together, these observations indicate that ETOH has unique effects on fetal IGF physiology compared with previously studied models of fetal growth retardation. Alterations in the expression and levels of IGFs and IGFBPs may contribute to ETOH‐induced fetal growth deficiency.

AbstractWidespread adoption of regenerative agriculture practices is an integral part of the US plan to achieve net‐zero greenhouse gas emissions by 2050. National incentives have particularly increased for the adoption of cover crops (CCs), which have presumably large carbon (C) sequestration potential. However, assessments of national CC climate benefits have not fully considered regional variability, changing C sequestration rates over time, and potential N2O trade‐offs. Using the DayCent soil biogeochemical model and current national survey data, we estimate CC climate change mitigation potential to be 39.0 ± 24.1 Mt CO2e year−1, which is 45%–65% lower than previous estimates, with large uncertainty attributed to N2O impacts. Three‐fourths of this climate change mitigation potential is concentrated in the North Central, Southern Great Plains and Lower Mississippi regions. Public investment should be focused in these regions to maximize CC climate benefits, but the national contribution of CC to emissions targets may be lower than previously anticipated.

Abstract A rising interest in battery systems for various applications needs a deep understanding of the system performance for technical and economical optimization. The electrical system behavior and the energy efficiency of two different Li-ion battery systems are presented in this paper. Both systems are designed for operation in a virtual storage plant with respect to the primary control reserve. The efficiency of both systems is analyzed including the battery, the power electronics and auxiliary units. Based on the overall energy efficiency of the electrical storage systems, a model is developed to show the impact on the operation and standby behavior. This model is transferred in a simulation framework investigating the application of battery systems towards primary control reserve. Real frequency data of continental European transmission system are used to simulate the behavior of the two systems in order to determine energy losses during operation. Primary control reserve is one possible application as grid support service for battery systems and is under current discussion. In this new field of battery application energy losses are a limiting factor for economic and technical qualification. The simulation identifies strengths and weaknesses of the investigated systems based on the determined efficiencies, and the results support an optimized operation strategy.