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Atmospheric concentrations of the greenhouse gas nitrous oxide (N(2)O) have increased significantly since pre-industrial times owing to anthropogenic perturbation of the global nitrogen cycle, with animal production being one of the main contributors. Grasslands cover about 20 per cent of the temperate land surface of the Earth and are widely used as pasture. It has been suggested that high animal stocking rates and the resulting elevated nitrogen input increase N(2)O emissions. Internationally agreed methods to upscale the effect of increased livestock numbers on N(2)O emissions are based directly on per capita nitrogen inputs. However, measurements of grassland N(2)O fluxes are often performed over short time periods, with low time resolution and mostly during the growing season. In consequence, our understanding of the daily and seasonal dynamics of grassland N(2)O fluxes remains limited. Here we report year-round N(2)O flux measurements with high and low temporal resolution at ten steppe grassland sites in Inner Mongolia, China. We show that short-lived pulses of N(2)O emission during spring thaw dominate the annual N(2)O budget at our study sites. The N(2)O emission pulses are highest in ungrazed steppe and decrease with increasing stocking rate, suggesting that grazing decreases rather than increases N(2)O emissions. Our results show that the stimulatory effect of higher stocking rates on nitrogen cycling and, hence, on N(2)O emission is more than offset by the effects of a parallel reduction in microbial biomass, inorganic nitrogen production and wintertime water retention. By neglecting these freeze-thaw interactions, existing approaches may have systematically overestimated N(2)O emissions over the last century for semi-arid, cool temperate grasslands by up to 72 per cent.

Alpine grasslands on the Qinghai-Tibetan Plateau are sensitive and vulnerable to climate change and human activities. Climate warming and overgrazing have already caused degradation in a large fraction of alpine grasslands on this plateau. However, it remains unclear how human activities (mainly livestock grazing) regulates vegetation dynamics under climate change. Here, alpine grassland productivity (substituted with the normalized difference vegetation index, NDVI) is hypothesized to vary in a nonlinear trajectory to follow climate fluctuations and human disturbances. With generalized additive mixed modelling (GAMM) and residual-trend (RESTREND) analysis together, both magnitude and direction of climatic (in terms of temperature, precipitation, and radiation) and anthropogenic impacts on NDVI variation were examined across alpine meadows, steppes, and desert-steppes on the Qinghai-Tibetan Plateau. The results revealed that accelerating warming and greening, respectively, took place in 76.2% and 78.8% of alpine grasslands on the Qinghai-Tibetan Plateau. The relative importance of temperature, precipitation, and radiation impacts was comparable, between 20.4% and 24.8%, and combined to explain 66.2% of NDVI variance at the pixel scale. The human influence was strengthening and weakening, respectively, in 15.5% and 14.3% of grassland pixels, being slightly larger than any sole climatic variable across the entire plateau. Anthropogenic and climatic factors can be in opposite ways to affect alpine grasslands, even within the same grassland type, likely regulated by plant community assembly and species functional traits. Therefore, the underlying mechanisms of how plant functional diversity regulates nonlinear ecosystem response to climatic and anthropogenic stresses should be carefully explored in the future.

Abstract Since the adoption of the Kyoto protocol in 1997 and its entry into force in 2005, as well its aftermath such as the Doha amendment and Paris agreement, national policies have become more conscious of the usage of clean energy, mostly the different forms of renewable energy and nuclear energy. Ratifying countries and signatories had committed themselves to binding targets for the reduction of greenhouse emissions by 8% with respect to 1990 levels until 2012, also based on the particular contribution to global emissions from each country. This paper examines the integrational properties of clean energy consumption from eight emerging economies which are also high greenhouse gas emitters. The empirical results show that the clean energy consumption is stationarity for Brazil and Philippines by using a quantile unit root test without smooth breaks (Koenker and Xiao, 2004). However, after capturing the smooth breaks (Bahmani-Oskooee et al., 2018), we find the clean energy consumption of China, Pakistan and Thailand are stationary. The time-varying deterministic trend with smooth breaks is more fitted to the path of clean energy consumption in comparison to the deterministic trend without smooth breaks. The paper suggests economic insights useful for policy making.

Biological infestations in forests, e.g. the insect outbreaks, have been shown as favoured by future climate change trends. In Europe, the European spruce bark beetle (Ips typographus L.) is one of the main agents causing substantial economic disturbances in forests. Therefore, studies on spatio-temporal characterization of the area affected by bark beetle are of major importance for rapid post-attack management. We aimed at spatially detecting damage classes by combining multidate remote sensing data and a non-parametric classification. As study site served a part of the Bavarian Forest National Park (Germany). For the analysis, we used 10 geometrically rectified scenes of Landsat and SPOT sensors in the period between 2001 and 2011. The main objective was to explore the potential of medium-resolution data for classifying the attacked areas. A further aim was to explore if the temporally adjacent infested areas are able to be separated. The random forest (RF) model was applied using the reference data drawn from high-resolution aerial imagery. The results indicate that the sufficiently large patches of visually identifiable damage classes can be accurately separated from non-attacked areas. In contrast to those, the other mortality classes (current year, current year 1 and current year 2 infested classes) were mostly classified with higher commission or omission errors as well as higher classification biases. The available medium-resolution satellite images, combined with properly acquired reference data, are concluded to be adequate tools to map area-based infestations at advanced stages. However, the quality of reference data, the size of infested patches and the spectral resolution of remotely sensed data are the decisive factors in case of smaller areas. Further attempts using auxiliary height information and spatially enhanced data may refine such an approach.

A cal. 20-year-resolution pollen record from Gonghai Lake presented the detailed process of mountain vegetation succession and East Asian Summer Monsoon (EASM) changes since the last deglaciation in Shanxi Province, North China. Modern vegetation distribution and lake surface pollen assemblages suggested that the fossil pollen mainly came from local and surrounding vegetation in Gonghai Lake, which reflected the elevational changes of plant communities in study area. From 14,700 to 11,100 cal. yr BP, open forests and mountain meadows dominated by shrubs and herbaceous species in surrounding area, suggesting a weak EASM with less precipitation. In the period between 11,100 and 7300 cal. yr BP, bushwoods and grasses were gradually replaced by mixed broadleaf-conifer forest, first developed by pioneer species of Betula and Populus and then replaced by Picea, Pinus, and Quercus, implying an enhanced EASM and increased temperature and precipitation. During the period of 7300–5000 cal. yr BP, warm-fitted trees became expanded and widespread, indicating a climax community of mixed broadleaf-conifer forest and warm and humid climate with higher temperature and sufficient precipitation and the strongest period of EASM. From 5000 to 1600 cal. yr BP, Pinus pollen increased, but Quercus pollen decreased, showing the breakup of the climax community and the recession of the EASM. Since 1600 cal. yr BP, under the threats of land reclamation and deforestation, forest cover sharply decreased, and mountain grass lands were developed. The EASM changes inferred from pollen record of Gonghai Lake were asynchronous to the oxygen isotope records of stalagmites from southern China. We suggest that the existence of remnant Northern Hemisphere ice sheets and relative low sea levels might hampered the northward penetration of the EASM in early Holocene, which caused the maximum monsoon precipitation to reach northern China until mid-Holocene.

AbstractThis study aimed to identify drought‐mediated differences in amino nitrogen (N) composition and content of xylem and phloem in trees having different symbiotic N2‐fixing bacteria. Under controlled water availability, 1‐year‐old seedlings ofRobinia pseudoacacia(nodules withRhizobium),Hippophae rhamnoides(symbiosis withFrankia) andBuddleja alternifolia(no such root symbiosis) were exposed to control, medium drought and severe drought, corresponding soil water content of 70–75%, 45–50% and 30–35% of field capacity, respectively. Composition and content of amino compounds in xylem sap and phloem exudates were analysed as a measure of N nutrition. Drought strongly reduced biomass accumulation in all species, but amino N content in xylem and phloem remained unaffected only inR. pseudoacacia. InH. rhamnoidesandB. alternifolia, amino N in phloem remained constant, but increased in xylem of both species in response to drought. There were differences in composition of amino compounds in xylem and phloem of the three species in response to drought. Proline concentrations in long‐distance transport pathways of all three species were very low, below the limit of detection in phloem ofH. rhamnoidesand in phloem and xylem ofB. alternifolia. Apparently, drought‐mediated changes in N composition were much more connected with species‐specific changes in C:N ratios. Irrespective of soil water content, the two species with root symbioses did not show similar features for the different types of symbiosis, neither in N composition nor in N content. There was no immediate correlation between symbiotic N fixation and drought‐mediated changes in amino N in the transport pathways.

Ten of 34 tested duckweed clones showed relatively higher salt tolerance. Salinity stress induced high level of starch accumulation in these clones, making them potential feedstock candidates for biofuel production. Duckweeds are promising as a new generation of crop plants that requires minimal input while providing fast biomass production. Two important traits of interest that can impact on the economic viability of this system are their sensitivity to salt and the starch content of the harvested duckweed. We have surveyed 33 strains of duckweed selected from across all 5 genera and amongst 13 species to quantify the natural variance of these traits. We found that there are large ranges of intraspecific variations in salt tolerance, while all species examined accumulated more starch in response to the initial stages of salt stress. However, the magnitude of the change in starch content varied widely between strains. Our results suggest that specific duckweed clones can be cultivated under relatively saline conditions, while increasing salt in the medium before harvesting could be used to increase starch in duckweed biomass for bioethanol production.