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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Mohamed S. Badawi; Bohaysa A. Salem; Abouzeid A. Thabet; Mohamed. A. Elzaher; +1 Authors

    The present work used the efficiency transfer method used to calculate the full energy peak efficiency (FEPE) curves of the (2“*2” & 3“*3”) NaI (Tl) detectors based on the effective solid angle subtended between the source and the detector. The study covered the effect of the self attenuation coefficient of the source matrix (with a radius greater than the detector's radius) on the detector efficiency. 152 An Eu aqueous radioactive source covering the energy range from 121.78 keV up to 1408.01 keV was used. In this study an empirical formula was deduced to calculate the difference between the measured and the calculated efficiencies [without self attenuation] at low and high energy regions. A proper balance between the measured and calculated efficiencies [with self attenuation] was achieved with discrepancies less than 3%, while reaching 39% for calculating values [without self attenuation] due to working with large sources, or for low photon energies.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Nuclear Engineering ...arrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Nuclear Engineering and Technology
    Article . 2014 . Peer-reviewed
    License: CC BY NC ND
    Data sources: Crossref
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Nuclear Engineering and Technology
    Article
    License: CC BY NC ND
    Data sources: UnpayWall
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Nuclear Engineering and Technology
    Article . 2014
    License: CC BY NC ND
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Nuclear Engineering ...arrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Nuclear Engineering and Technology
      Article . 2014 . Peer-reviewed
      License: CC BY NC ND
      Data sources: Crossref
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Nuclear Engineering and Technology
      Article
      License: CC BY NC ND
      Data sources: UnpayWall
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Nuclear Engineering and Technology
      Article . 2014
      License: CC BY NC ND
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      This Research product is the result of merged Research products in OpenAIRE.

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Haiyan Li; Suguru Tsuchimoto; Kyuya Harada; Mariko Yamasaki; +16 Authors

    Jatropha curcas L. (Jatropha), une espèce arbustive de la famille des Euphorbiaceae, a été reconnue comme une usine de biocarburants prometteuse pour réduire les émissions de gaz à effet de serre. Cependant, les récentes tentatives de culture commerciale en Afrique et en Asie ont échoué en raison de la faible productivité. Il est important d'élucider la diversité génétique et la relation dans les ressources génétiques mondiales de Jatropha pour la sélection de meilleurs cultivars commerciaux. Ici, la diversité génétique a été analysée en utilisant 246 accessions de la Méso-Amérique, de l'Afrique et de l'Asie, sur la base de 59 marqueurs de répétition de séquence simple et de huit marqueurs de polymorphisme d'insertion à base de rétrotransposons. Nous avons constaté que le Chiapas central du Mexique possède les ressources génétiques les plus diverses, et la dépression centrale du Chiapas pourrait être le centre d'origine. Nous avons identifié trois groupes génétiques en Méso-Amérique, dont la répartition a révélé un cline géographique distinct. L'un d'eux consiste principalement en des adhésions du centre du Chiapas. Cela suggère qu'il représente le groupe génétique d'origine. Nous avons trouvé deux accessions Veracruz dans un autre groupe, dont les ancêtres pourraient être expédiés du port de Veracruz vers l'Ancien Monde, pour être la source de tous les Jatropha africains et asiatiques. Nos résultats suggèrent la sélection humaine qui a causé une faible productivité en Afrique et en Asie, ainsi que des stratégies de sélection pour améliorer le jatropha africain et asiatique. Les cultivars améliorés dans la productivité contribueront à développer la culture commerciale de masse du Jatropha en Afrique et en Asie pour augmenter la production de biocarburants, et enfin soutiendront la lutte contre le changement climatique. Jatropha curcas L. (Jatropha), una especie de arbusto de la familia Euphorbiaceae, ha sido reconocida como una planta de biocombustible prometedora para reducir las emisiones de gases de efecto invernadero. Sin embargo, los recientes intentos de cultivo comercial en África y Asia han fracasado debido a la baja productividad. Es importante dilucidar la diversidad genética y la relación en los recursos genéticos mundiales de Jatropha para la reproducción de mejores cultivares comerciales. Aquí, se analizó la diversidad genética mediante el uso de 246 accesiones de Mesoamérica, África y Asia, basadas en 59 marcadores de repetición de secuencia simple y ocho marcadores de polimorfismo de inserción basados en retrotransposones. Encontramos que el centro de Chiapas de México posee los recursos genéticos más diversos, y la Depresión Central de Chiapas podría ser el centro de origen. Identificamos tres grupos genéticos en Mesoamérica, cuya distribución reveló un clino geográfico distinto. Uno de ellos consiste principalmente en accesiones desde el centro de Chiapas. Esto sugiere que representa el grupo genético original. Encontramos dos accesiones de Veracruz en otro grupo, cuyos antepasados podrían ser enviados desde el Puerto de Veracruz al Viejo Mundo, para ser la fuente de toda la jatrofa africana y asiática. Nuestros resultados sugieren la selección humana que causó la baja productividad en África y Asia, y también estrategias de cría para mejorar la jatrofa africana y asiática. Los cultivos mejorados en la productividad contribuirán a expandir el cultivo comercial masivo de Jatropha en África y Asia para aumentar la producción de biocombustibles, y finalmente apoyarán en la batalla contra el cambio climático. Jatropha curcas L. (Jatropha), a shrub species of the family Euphorbiaceae, has been recognized as a promising biofuel plant for reducing greenhouse gas emissions. However, recent attempts at commercial cultivation in Africa and Asia have failed because of low productivity. It is important to elucidate genetic diversity and relationship in worldwide Jatropha genetic resources for breeding of better commercial cultivars. Here, genetic diversity was analyzed by using 246 accessions from Mesoamerica, Africa and Asia, based on 59 simple sequence repeat markers and eight retrotransposon-based insertion polymorphism markers. We found that central Chiapas of Mexico possesses the most diverse genetic resources, and the Chiapas Central Depression could be the center of origin. We identified three genetic groups in Mesoamerica, whose distribution revealed a distinct geographic cline. One of them consists mainly of accessions from central Chiapas. This suggests that it represents the original genetic group. We found two Veracruz accessions in another group, whose ancestors might be shipped from Port of Veracruz to the Old World, to be the source of all African and Asian Jatropha. Our results suggest the human selection that caused low productivity in Africa and Asia, and also breeding strategies to improve African and Asian Jatropha. Cultivars improved in the productivity will contribute to expand mass commercial cultivation of Jatropha in Africa and Asia to increase biofuel production, and finally will support in the battle against the climate change. Jatropha curcas L. (Jatropha)، وهو نوع من الشجيرات من عائلة Euphorbiaceae، تم الاعتراف به كمصنع واعد للوقود الحيوي لتقليل انبعاثات غازات الدفيئة. ومع ذلك، فشلت المحاولات الأخيرة للزراعة التجارية في أفريقيا وآسيا بسبب انخفاض الإنتاجية. من المهم توضيح التنوع الوراثي والعلاقة في الموارد الوراثية للجاتروفا في جميع أنحاء العالم لتربية أصناف تجارية أفضل. هنا، تم تحليل التنوع الجيني باستخدام 246 ملحقًا من أمريكا الوسطى وأفريقيا وآسيا، استنادًا إلى 59 علامة تكرار تسلسل بسيطة وثمانية علامات تعدد أشكال الإدراج القائمة على الترانسبروسون. وجدنا أن وسط تشياباس في المكسيك يمتلك الموارد الجينية الأكثر تنوعًا، ويمكن أن يكون الكساد المركزي في تشياباس هو مركز المنشأ. حددنا ثلاث مجموعات وراثية في أمريكا الوسطى، والتي كشف توزيعها عن سلالة جغرافية متميزة. يتكون أحدها بشكل أساسي من المنضمين من وسط تشياباس. هذا يشير إلى أنه يمثل المجموعة الوراثية الأصلية. وجدنا انضمامين لفيراكروز في مجموعة أخرى، قد يتم شحن أسلافهم من ميناء فيراكروز إلى العالم القديم، ليكونوا مصدر كل الجاتروفا الأفريقية والآسيوية. تشير نتائجنا إلى الانتقاء البشري الذي تسبب في انخفاض الإنتاجية في أفريقيا وآسيا، وكذلك استراتيجيات التكاثر لتحسين الجاتروفا الأفريقية والآسيوية. ستساهم الأصناف المحسنة في الإنتاجية في توسيع الزراعة التجارية الجماعية للجاتروفا في إفريقيا وآسيا لزيادة إنتاج الوقود الحيوي، وأخيرًا ستدعم المعركة ضد تغير المناخ.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Frontiers in Plant S...arrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Frontiers in Plant Science
    Article . 2017 . Peer-reviewed
    Data sources: Crossref
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Frontiers in Plant Science
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    PubMed Central
    Other literature type . 2017
    License: CC BY
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Frontiers in Plant Science
    Article . 2017
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    https://dx.doi.org/10.60692/6r...
    Other literature type . 2017
    Data sources: Datacite
    https://dx.doi.org/10.60692/c2...
    Other literature type . 2017
    Data sources: Datacite
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Frontiers in Plant S...arrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Frontiers in Plant Science
      Article . 2017 . Peer-reviewed
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      Other literature type . 2017
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Frontiers in Plant Science
      Article . 2017
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      https://dx.doi.org/10.60692/6r...
      Other literature type . 2017
      Data sources: Datacite
      https://dx.doi.org/10.60692/c2...
      Other literature type . 2017
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Fahad M. Alminderej; Zeineb Hamden; Yassine El-Ghoul; Bechir Hammami; +2 Authors

    Given crude oil prices and their environmental impacts, the use of sustainable renewable alternative energies such as biofuels is rapidly progressing in numerous countries. Among biofuels, bioethanol is a renewable and clean fuel that can be obtained from the fermentation of several raw agricultural materials, including date fruit. However, the low product yield, mainly due to the low-grade nutrient content, limits its use as a promising alternative biofuel. This current study investigated bioethanol production from date by-products in Saudi Arabia and examined the impact of calcium and nitrogen sources added at different concentrations (0 to 1 g/L) on the productivity and ethanol concentration using Saccharomyces cerevisiae. Yeast extracts and ammonium chloride (NH4Cl) were tested as nitrogen sources for bioethanol fermentation from date juice. Calcium chloride (CaCl2) and calcium carbonate (CaCO3) were evaluated as calcium sources for the same purpose mentioned above. The results showed that both calcium and nitrogen sources improved ethanol production efficiencies. The addition of calcium sources such as CaCl2 at 0.4 g/L resulted in maximum ethanol concentration (41.5 ± 0.85 g/L) and the highest productivity of 0.511 g/L/h. Thus, an increase of 31.3% compared to the control sample was acquired. Ammonium chloride was found to be the best nitrogen supplement among them. Indeed, supplementing the fermentation medium with 1 g/L NH4Cl gave an optimal ethanol concentration and productivity, reaching more than 65 g/L and 0.83 g/L/h, respectively. This is an increase of 106.6%. The functional group of ethanol (C2H5OH) for all the elaborated samples was confirmed by Fourier-transform infrared spectroscopy (FTIR) and NMR analyses. Moreover, the results confirmed the high quality and purity of the bioethanol products. Thus, the “Khodhari” date variety of low market value is a privileged substrate for industrial bioethanol production. For this reason, a proposed flow diagram of a designed plant for bioethanol industrialization is provided and detailed.

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    Fermentation
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      Fermentation
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    Authors: Muziri Mugwanya; Mahmoud A. O. Dawood; Fahad Kimera; Hani Sewilam;

    The increasing global population has led to an increase in food demand; consequently, aquaculture is one of the food production sectors that has offered opportunities to alleviate hunger, malnutrition, and poverty. However, the development of a sustainable aquaculture industry has been hindered by the limited availability of natural resources as well as its negative impact on the surrounding environment. Hence, there is an urgent need to search for better aquacultural production systems that, despite their high productivity and profitability, utilize fewer resources such as water, energy, land, and capital in conjunction with a negligible impact on the environment. Biofloc technology (BFT) is one of the most exciting and promising sustainable aquaculture systems; it takes into account the intensive culture of aquatic species, zero water exchange, and improved water quality as a result of beneficial microbial biomass activity, which, at the same time, can be utilized as a nutritious aquaculture feed, thus lowering the costs of production. Furthermore, BFT permits the installation of integrated multi-trophic aquaculture (IMTA) systems in which the wastes of one organism are utilized as feed by another organism, without a detrimental effect on co-cultured species. This review, therefore, highlights the basics of BFT, factors associated with BFT for the successful production of aquatic species, the significance of this food production system for the sustainable production of economically important aquatic species, its economic aspects, drawbacks, limitations, and recommended management aspects for sustainable aquaculture.

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  • Authors: Yukihiro Tashiro; Kenji Sonomoto; Mohamed Ali Abdel-Rahman;

    Fermentative production of optically pure lactic acid has roused interest among researchers in recent years due to its high potential for applications in a wide range of fields. More specifically, the sharp increase in manufacturing of biodegradable polylactic acid (PLA) materials, green alternatives to petroleum-derived plastics, has significantly increased the global interest in lactic acid production. However, higher production costs have hindered the large-scale application of PLA because of the high price of lactic acid. Therefore, reduction of lactic acid production cost through utilization of inexpensive substrates and improvement of lactic acid production and productivity has become an important goal. Various methods have been employed for enhanced lactic acid production, including several bioprocess techniques facilitated by wild-type and/or engineered microbes. In this review, we will discuss lactic acid producers with relation to their fermentation characteristics and metabolism. Inexpensive fermentative substrates, such as dairy products, food and agro-industrial wastes, glycerol, and algal biomass alternatives to costly pure sugars and food crops are introduced. The operational modes and fermentation methods that have been recently reported to improve lactic acid production in terms of concentrations, yields, and productivities are summarized and compared. High cell density fermentation through immobilization and cell-recycling techniques are also addressed. Finally, advances in recovery processes and concluding remarks on the future outlook of lactic acid production are presented.

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    Authors: Nermin A. El Semary;

    Iron limitation in vast water bodies has been linked to decreased algal productivity, despite different iron-acquiring mechanisms, and the presence of ferritin in many algal species that act as an iron internal reservoir. Therefore, iron fertilization has been proposed to increase algal biomass and photosynthesis. This, in turn, will reduce carbon dioxide in the atmosphere and increase oxygen, thereby decreasing global warming, and achieving ecological balance. In addition, algal proliferation will hopefully lead to enhancement in biodiversity, Biological pump, fish productivity and, subsequently marine food industry. Many climate geoengineering experiments in the form of ocean iron fertilization have been conducted globally in order to achieve such a purpose. However, reservations remain as the outcomes are not as promising as were previously expected. As the temporal and spatial scales of iron fertilization experiments are limited, the effects on fish productivity remain speculative. On the other hand, side effects were also recorded. The main purpose of iron fertilization, for carbon dioxide sequestration and global warming mitigation, still remains to be fully realized and verified. Several improvements and future modifications are suggested, and legal issues are discussed in this review.

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    Sustainability
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    Authors: Shymaa Ryhan Bashandy; Mohamed Hemida Abd-Alla; Ghada Abd-Elmonsef Mahmoud;

    Abstract Aim This study aims to use fermentation waste of ethanol production (solid and liquid) for riboflavin and recycling of bacterial biomass as biofertilizers to enhance the growth of some oily crop plants. Methods and Results Out of 10 yeast isolates from fresh milk, Clavispora lusitaniae ASU 33 (MN583181) was able to ferment different concentrations of glucose (2.5%, 5%, 7.5%, 10%, 15% and 20%) into ethanol with high efficiency at 10%. Among seven non-Lactobacillus bacterial isolates recovered from cheese samples, two bacterial isolates Bacillus subtlis-SR2 (MT002768) and Novosphingobium panipatense-SR3 (MT002778) were selected for their high riboflavin production. Different media (control medium, fermentation waste medium and a mixture of the fermentation waste medium and control medium [1:1]) were used for riboflavin production. These media were inoculated by a single or mixture of B. subtlis-SR2, N. panipatense-SR3. The addition of the waste medium of ethanol production to the control medium (1:1) had a stimulatory effect on riboflavin production whether inoculated with either a single strain or a mixture of B. subtlis-SR2 and N. panipatense-SR3. A mixture of fermentation waste and control media inoculated with N. panipatense produced a high riboflavin yield in comparison with other media. Inoculation of Zea mays and Ocimum basilicum plants with either the bacterial biomass waste of riboflavin production (B. subtlis or N. panipatense) or a mixture of B. subtlis and N. panipatense) shows a stimulatory effect on the plant growth in comparison with control (uninoculated plants). Conclusions These results demonstrate the possibility of minimizing the cost of riboflavin and biofertilizer manufacturing via interlinking ethanol and riboflavin with the biofertilizer production technology. Significance and Impact of Study This study outlines the methods of evaluating the strength of spent media by applying procedures developed in the vitamin production industries. Furthermore, bacterial biomass waste can act as an environmentally friendly alternative for agrochemicals.

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    Journal of Applied Microbiology
    Article . 2022 . Peer-reviewed
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    Authors: Seyyed Reza Sobhani; Nasrin Omidvar; Zahra Abdollahi; Ayoub Al-Jawaldeh;

    The need for a shift in diet toward a more sustainable one has reached an urgency in certain regions, including Iran, due to more rapid climate change and a higher level of vulnerability. This study was undertaken to identify and summarize available data on changes required in the current Iranian diet to make it more sustainable and the extent to which current policies in the country have addressed such a shift. In this study, PubMed, Scopus, and Web of science, as well as Iranian scientific search engines, including Scientific Information Database and Magiran, were systematically searched from January 1990 to July 2021. A total of 11 studies and policy analyses were included in this study. Based on the findings, moving Iranian diet toward sustainability will require increase in consumption of dairy, fruits, vegetables, cereals, poultry, and legumes and decrease in consumption of bread, rice, pasta, red meat, eggs, fats, sugars, and sweets. There has been a great deal of effort and investment on policies and strategies to decrease the amount of sugar, salt, and fat (specifically trans-fatty acids) in the Iranian diet, which makes it more sustainable healthwise. Several policies and programs have been implemented to tackle non-communicable diseases (NCDs) by reducing access to unhealthy foods, which is in line with health dimension of a sustainable diet. However, there is almost no direct address to ecological aspect of sustainable diet in the food and nutrition policy documents in the ccountry. Development of an enabling environment to a sustainable diet will require policy and actions to improve public awareness, support study to provide evidence and identify possible alternatives, and plan and implement interventions/programs to promote and facilitate healthy and sustainable diets.

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    Frontiers in Nutrition
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    PubMed Central
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    Frontiers in Nutrition
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    https://dx.doi.org/10.60692/rf...
    Other literature type . 2021
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    Authors: R. Z. Sayyed; S. S. Shaikh; S. J. Wani; Md Tabish Rehman; +3 Authors

    The present study was aimed to evaluate the suitability of agro-wastes and crude vegetable oils for the cost-effective production of poly-β-hydroxybutyrate (PHB), to evaluate growth kinetics and PHB production in Alcaligenes faecalis RZS4 and Pseudomonas sp. RZS1 with these carbon substrates and to study the biodegradation of PHB accumulated by these cultures. Alcaligenes faecalis RZS4 and Pseudomonas sp. RZS1 accumulates higher amounts of PHB corn (79.90% of dry cell mass) and rice straw (66.22% of dry cell mass) medium respectively. The kinetic model suggests that the Pseudomonas sp. RZS1 follows the Monod model more closely than A. faecalis RZS4. Both the cultures degrade their PHB extract under the influence of PHB depolymerase. Corn waste and rice straw appear as the best and cost-effective substrates for the sustainable production of PHB from Alcaligenes faecalis RZS4 and Pseudomonas sp. RZS1. The biopolymer accumulated by these organisms is biodegradable in nature. The agro-wastes and crude vegetable oils are good and low-cost sources of nutrients for the growth and production of PHB and other metabolites. Their use would lower the production cost of PHB and the low-cost production will reduce the sailing price of PHB-based products. This would promote the large-scale commercialization and popularization of PHB as an ecofriendly bioplastic/biopolymer.

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    Molecules
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    Authors: Sang Un Oh; Reda A.I. Abou-Shanab; Byong-Hun Jeon; Nagah M.A. Hassanin; +4 Authors

    La production de biodiesel à partir de microalgues dépend de la biomasse algale et de la teneur en lipides. La production de biomasse et l'accumulation de lipides sont limitées par plusieurs facteurs dans lesquels les nutriments jouent un rôle clé. Nous avons étudié les influences des micronutriments sur la biomasse et la teneur en lipides de Micractinium pusillum GU732425 cultivé en milieu basal gras (BBM). La biomasse sèche moyenne de la souche de microalgues dans le milieu témoin a atteint 0,34 ± 0,01 g /L, tandis que le doublement (2X) des niveaux de concentration en Mn et Cu a augmenté la biomasse sèche à 0,38 ± 0,01 et 0,37 ± 0,02 g /L, respectivement.M. pusillum cultivé en milieu témoin avait une biomasse de 0,82 ± 0,05 g/L et une productivité lipidique de 0,33 ± 0,02 g/L après 17 jours de culture.M. pusillum cultivé en BBM avec 4X Mn ou 4X Cu produisait plus de biomasse (1,25 ± 0,01 ou 1,28 ± 0,04 g pc/L) et de productivité lipidique (0,45±0,04 ou 0,47±0,05g/L), respectivement.M. pusillum cultivé dans différents milieux de croissance avait des compositions d'acides gras comprenant principalement des acides linoléique (49-54%), palmitique (24-29%), linolénique (16-22 %) et les acides oléiques (2-5 %). Ces résultats peuvent être utilisés pour maximiser la production de biomasse et de lipides de microalgues dans des photobioréacteurs conçus de manière optimale. La producción de biodiesel a partir de microalgas depende de la biomasa de algas y el contenido de lípidos. Tanto la producción de biomasa como la acumulación de lípidos están limitadas por varios factores en los que los nutrientes juegan un papel clave. Investigamos las influencias de los micronutrientes en la biomasa y el contenido de lípidos de Micractinium pusillum GU732425 cultivado en medios basales en negrita (BBM). La biomasa seca promedio de la cepa de microalgas en el medio de control alcanzó 0.34 ± 0.01 g /L, mientras que duplicando (2X) los niveles de concentración de Mn y Cu aumentó la biomasa seca a 0.38 ± 0.01 y 0.37 ± 0.02 g /L, respectivamente. M. pusillum cultivado en medio de control tuvo una biomasa de 0.82 ± 0.05 g/L y una productividad lipídica de 0.33 ± 0.02 g/L después de 17 días de cultivo. El alga cultivada en BBM con 4X Mn o 4X Cu produjo más biomasa (1.25 ± 0.01 o 1.28 ± 0.04 g dw/L) y productividad lipídica (0.45±0.04 o 0.47±0.05g/L), respectivamente. M. pusillum cultivado en diferentes medios de crecimiento tuvo composiciones de ácidos grasos que comprenden principalmente linoleico (49-54%), palmítico (24-29%), linolénico (16-22%) y ácidos oleicos (2-5%). Estos resultados se pueden utilizar para maximizar la producción de biomasa y lípidos de microalgas en fotobiorreactores diseñados de manera óptima. Biodiesel production from microalgae depends on the algal biomass and lipid content.Both biomass production and lipid accumulation are limited by several factors in which nutrients play a key role.We investigated the influences of micronutrients on biomass, and lipid content of Micractinium pusillum GU732425 cultivated in bold basal media (BBM).The average dry biomass of microalgal strain in control medium reached 0.34 ± 0.01 g /L, while doubling (2X) the levels of Mn and Cu concentration increased the dry biomass to 0.38 ± 0.01 and 0.37 ± 0.02 g /L, respectively.M. pusillum cultivated in control medium had a biomass of 0.82 ± 0.05 g/L and a lipid productivity of 0.33 ± 0.02 g/L after 17 day cultivation.The alga cultivated in BBM with 4X Mn or 4X Cu produced more biomass (1.25 ± 0.01 or 1.28 ± 0.04 g dw/L) and lipid productivity (0.45±0.04 or 0.47±0.05g/L), respectively.M. pusillum cultivated in different growth media had fatty acid compositions mainly comprising linoleic (49-54%), palmitic (24-29%), linolenic (16-22%), and oleic acids (2-5%).These results can be used to maximize the production of microalgal biomass and lipids in optimally designed photobioreactors. يعتمد إنتاج الديزل الحيوي من الطحالب الدقيقة على الكتلة الحيوية للطحالب ومحتوى الدهون. كل من إنتاج الكتلة الحيوية وتراكم الدهون محدودان بعدة عوامل تلعب فيها العناصر الغذائية دورًا رئيسيًا. لقد حققنا في تأثيرات المغذيات الدقيقة على الكتلة الحيوية، وزاد محتوى الدهون من Micractinium pusillum GU732425 المزروع في الوسائط القاعدية الجريئة (BBM). بلغ متوسط الكتلة الحيوية الجافة لسلالة الطحالب الدقيقة في وسط التحكم 0.34 ± 0.01 جم /لتر، بينما أدى مضاعفة (2X) مستويات تركيز Mn و Cu إلى زيادة الكتلة الحيوية الجافة إلى 0.38 ± 0.01 و 0.37 ± 0.02 جم /لتر، على التوالي. كان لبوسيلوم المزروع في وسط التحكم كتلة حيوية تبلغ 0.82 ± 0.05 جم/لتر وإنتاجية دهنية تبلغ 0.33 ± 0.02 جم/لتر بعد 17 يومًا من الزراعة. الطحالب المزروعة في BBM مع 4X Mn أو 4X Cu أنتجت المزيد من الكتلة الحيوية (1.25 ± 0.01 أو 1.28 ± 0.04 جم dw/L) وإنتاجية دهنية (0.45±0.04 أو 0.47±0.05جم/لتر)، على التوالي. كان لبوسيلوم المزروع في وسائط نمو مختلفة يحتوي على تركيبات الأحماض الدهنية التي تتكون أساسًا من اللينوليك (49-54 ٪)، النخيل (24-29 ٪)، اللينولينيك (16-22 ٪)، وأحماض الأوليك (2-5 ٪). يمكن استخدام هذه النتائج لزيادة إنتاج الكتلة الحيوية للطحالب الدقيقة والدهون في المفاعلات الحيوية الضوئية المصممة على النحو الأمثل.

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    African Journal of Biotechnology
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Mohamed S. Badawi; Bohaysa A. Salem; Abouzeid A. Thabet; Mohamed. A. Elzaher; +1 Authors

    The present work used the efficiency transfer method used to calculate the full energy peak efficiency (FEPE) curves of the (2“*2” & 3“*3”) NaI (Tl) detectors based on the effective solid angle subtended between the source and the detector. The study covered the effect of the self attenuation coefficient of the source matrix (with a radius greater than the detector's radius) on the detector efficiency. 152 An Eu aqueous radioactive source covering the energy range from 121.78 keV up to 1408.01 keV was used. In this study an empirical formula was deduced to calculate the difference between the measured and the calculated efficiencies [without self attenuation] at low and high energy regions. A proper balance between the measured and calculated efficiencies [with self attenuation] was achieved with discrepancies less than 3%, while reaching 39% for calculating values [without self attenuation] due to working with large sources, or for low photon energies.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Nuclear Engineering ...arrow_drop_down
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Nuclear Engineering and Technology
    Article . 2014 . Peer-reviewed
    License: CC BY NC ND
    Data sources: Crossref
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Nuclear Engineering and Technology
    Article
    License: CC BY NC ND
    Data sources: UnpayWall
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Nuclear Engineering and Technology
    Article . 2014
    License: CC BY NC ND
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Nuclear Engineering ...arrow_drop_down
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Nuclear Engineering and Technology
      Article . 2014 . Peer-reviewed
      License: CC BY NC ND
      Data sources: Crossref
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Nuclear Engineering and Technology
      Article
      License: CC BY NC ND
      Data sources: UnpayWall
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      Nuclear Engineering and Technology
      Article . 2014
      License: CC BY NC ND
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Haiyan Li; Suguru Tsuchimoto; Kyuya Harada; Mariko Yamasaki; +16 Authors

    Jatropha curcas L. (Jatropha), une espèce arbustive de la famille des Euphorbiaceae, a été reconnue comme une usine de biocarburants prometteuse pour réduire les émissions de gaz à effet de serre. Cependant, les récentes tentatives de culture commerciale en Afrique et en Asie ont échoué en raison de la faible productivité. Il est important d'élucider la diversité génétique et la relation dans les ressources génétiques mondiales de Jatropha pour la sélection de meilleurs cultivars commerciaux. Ici, la diversité génétique a été analysée en utilisant 246 accessions de la Méso-Amérique, de l'Afrique et de l'Asie, sur la base de 59 marqueurs de répétition de séquence simple et de huit marqueurs de polymorphisme d'insertion à base de rétrotransposons. Nous avons constaté que le Chiapas central du Mexique possède les ressources génétiques les plus diverses, et la dépression centrale du Chiapas pourrait être le centre d'origine. Nous avons identifié trois groupes génétiques en Méso-Amérique, dont la répartition a révélé un cline géographique distinct. L'un d'eux consiste principalement en des adhésions du centre du Chiapas. Cela suggère qu'il représente le groupe génétique d'origine. Nous avons trouvé deux accessions Veracruz dans un autre groupe, dont les ancêtres pourraient être expédiés du port de Veracruz vers l'Ancien Monde, pour être la source de tous les Jatropha africains et asiatiques. Nos résultats suggèrent la sélection humaine qui a causé une faible productivité en Afrique et en Asie, ainsi que des stratégies de sélection pour améliorer le jatropha africain et asiatique. Les cultivars améliorés dans la productivité contribueront à développer la culture commerciale de masse du Jatropha en Afrique et en Asie pour augmenter la production de biocarburants, et enfin soutiendront la lutte contre le changement climatique. Jatropha curcas L. (Jatropha), una especie de arbusto de la familia Euphorbiaceae, ha sido reconocida como una planta de biocombustible prometedora para reducir las emisiones de gases de efecto invernadero. Sin embargo, los recientes intentos de cultivo comercial en África y Asia han fracasado debido a la baja productividad. Es importante dilucidar la diversidad genética y la relación en los recursos genéticos mundiales de Jatropha para la reproducción de mejores cultivares comerciales. Aquí, se analizó la diversidad genética mediante el uso de 246 accesiones de Mesoamérica, África y Asia, basadas en 59 marcadores de repetición de secuencia simple y ocho marcadores de polimorfismo de inserción basados en retrotransposones. Encontramos que el centro de Chiapas de México posee los recursos genéticos más diversos, y la Depresión Central de Chiapas podría ser el centro de origen. Identificamos tres grupos genéticos en Mesoamérica, cuya distribución reveló un clino geográfico distinto. Uno de ellos consiste principalmente en accesiones desde el centro de Chiapas. Esto sugiere que representa el grupo genético original. Encontramos dos accesiones de Veracruz en otro grupo, cuyos antepasados podrían ser enviados desde el Puerto de Veracruz al Viejo Mundo, para ser la fuente de toda la jatrofa africana y asiática. Nuestros resultados sugieren la selección humana que causó la baja productividad en África y Asia, y también estrategias de cría para mejorar la jatrofa africana y asiática. Los cultivos mejorados en la productividad contribuirán a expandir el cultivo comercial masivo de Jatropha en África y Asia para aumentar la producción de biocombustibles, y finalmente apoyarán en la batalla contra el cambio climático. Jatropha curcas L. (Jatropha), a shrub species of the family Euphorbiaceae, has been recognized as a promising biofuel plant for reducing greenhouse gas emissions. However, recent attempts at commercial cultivation in Africa and Asia have failed because of low productivity. It is important to elucidate genetic diversity and relationship in worldwide Jatropha genetic resources for breeding of better commercial cultivars. Here, genetic diversity was analyzed by using 246 accessions from Mesoamerica, Africa and Asia, based on 59 simple sequence repeat markers and eight retrotransposon-based insertion polymorphism markers. We found that central Chiapas of Mexico possesses the most diverse genetic resources, and the Chiapas Central Depression could be the center of origin. We identified three genetic groups in Mesoamerica, whose distribution revealed a distinct geographic cline. One of them consists mainly of accessions from central Chiapas. This suggests that it represents the original genetic group. We found two Veracruz accessions in another group, whose ancestors might be shipped from Port of Veracruz to the Old World, to be the source of all African and Asian Jatropha. Our results suggest the human selection that caused low productivity in Africa and Asia, and also breeding strategies to improve African and Asian Jatropha. Cultivars improved in the productivity will contribute to expand mass commercial cultivation of Jatropha in Africa and Asia to increase biofuel production, and finally will support in the battle against the climate change. Jatropha curcas L. (Jatropha)، وهو نوع من الشجيرات من عائلة Euphorbiaceae، تم الاعتراف به كمصنع واعد للوقود الحيوي لتقليل انبعاثات غازات الدفيئة. ومع ذلك، فشلت المحاولات الأخيرة للزراعة التجارية في أفريقيا وآسيا بسبب انخفاض الإنتاجية. من المهم توضيح التنوع الوراثي والعلاقة في الموارد الوراثية للجاتروفا في جميع أنحاء العالم لتربية أصناف تجارية أفضل. هنا، تم تحليل التنوع الجيني باستخدام 246 ملحقًا من أمريكا الوسطى وأفريقيا وآسيا، استنادًا إلى 59 علامة تكرار تسلسل بسيطة وثمانية علامات تعدد أشكال الإدراج القائمة على الترانسبروسون. وجدنا أن وسط تشياباس في المكسيك يمتلك الموارد الجينية الأكثر تنوعًا، ويمكن أن يكون الكساد المركزي في تشياباس هو مركز المنشأ. حددنا ثلاث مجموعات وراثية في أمريكا الوسطى، والتي كشف توزيعها عن سلالة جغرافية متميزة. يتكون أحدها بشكل أساسي من المنضمين من وسط تشياباس. هذا يشير إلى أنه يمثل المجموعة الوراثية الأصلية. وجدنا انضمامين لفيراكروز في مجموعة أخرى، قد يتم شحن أسلافهم من ميناء فيراكروز إلى العالم القديم، ليكونوا مصدر كل الجاتروفا الأفريقية والآسيوية. تشير نتائجنا إلى الانتقاء البشري الذي تسبب في انخفاض الإنتاجية في أفريقيا وآسيا، وكذلك استراتيجيات التكاثر لتحسين الجاتروفا الأفريقية والآسيوية. ستساهم الأصناف المحسنة في الإنتاجية في توسيع الزراعة التجارية الجماعية للجاتروفا في إفريقيا وآسيا لزيادة إنتاج الوقود الحيوي، وأخيرًا ستدعم المعركة ضد تغير المناخ.

    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Frontiers in Plant S...arrow_drop_down
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Frontiers in Plant Science
    Article . 2017 . Peer-reviewed
    Data sources: Crossref
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    Frontiers in Plant Science
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    image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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    Other literature type . 2017
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    Frontiers in Plant Science
    Article . 2017
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    https://dx.doi.org/10.60692/6r...
    Other literature type . 2017
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    https://dx.doi.org/10.60692/c2...
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      image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/ Frontiers in Plant S...arrow_drop_down
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      Frontiers in Plant Science
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      https://dx.doi.org/10.60692/6r...
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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Fahad M. Alminderej; Zeineb Hamden; Yassine El-Ghoul; Bechir Hammami; +2 Authors

    Given crude oil prices and their environmental impacts, the use of sustainable renewable alternative energies such as biofuels is rapidly progressing in numerous countries. Among biofuels, bioethanol is a renewable and clean fuel that can be obtained from the fermentation of several raw agricultural materials, including date fruit. However, the low product yield, mainly due to the low-grade nutrient content, limits its use as a promising alternative biofuel. This current study investigated bioethanol production from date by-products in Saudi Arabia and examined the impact of calcium and nitrogen sources added at different concentrations (0 to 1 g/L) on the productivity and ethanol concentration using Saccharomyces cerevisiae. Yeast extracts and ammonium chloride (NH4Cl) were tested as nitrogen sources for bioethanol fermentation from date juice. Calcium chloride (CaCl2) and calcium carbonate (CaCO3) were evaluated as calcium sources for the same purpose mentioned above. The results showed that both calcium and nitrogen sources improved ethanol production efficiencies. The addition of calcium sources such as CaCl2 at 0.4 g/L resulted in maximum ethanol concentration (41.5 ± 0.85 g/L) and the highest productivity of 0.511 g/L/h. Thus, an increase of 31.3% compared to the control sample was acquired. Ammonium chloride was found to be the best nitrogen supplement among them. Indeed, supplementing the fermentation medium with 1 g/L NH4Cl gave an optimal ethanol concentration and productivity, reaching more than 65 g/L and 0.83 g/L/h, respectively. This is an increase of 106.6%. The functional group of ethanol (C2H5OH) for all the elaborated samples was confirmed by Fourier-transform infrared spectroscopy (FTIR) and NMR analyses. Moreover, the results confirmed the high quality and purity of the bioethanol products. Thus, the “Khodhari” date variety of low market value is a privileged substrate for industrial bioethanol production. For this reason, a proposed flow diagram of a designed plant for bioethanol industrialization is provided and detailed.

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    Fermentation
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      Fermentation
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    Authors: Muziri Mugwanya; Mahmoud A. O. Dawood; Fahad Kimera; Hani Sewilam;

    The increasing global population has led to an increase in food demand; consequently, aquaculture is one of the food production sectors that has offered opportunities to alleviate hunger, malnutrition, and poverty. However, the development of a sustainable aquaculture industry has been hindered by the limited availability of natural resources as well as its negative impact on the surrounding environment. Hence, there is an urgent need to search for better aquacultural production systems that, despite their high productivity and profitability, utilize fewer resources such as water, energy, land, and capital in conjunction with a negligible impact on the environment. Biofloc technology (BFT) is one of the most exciting and promising sustainable aquaculture systems; it takes into account the intensive culture of aquatic species, zero water exchange, and improved water quality as a result of beneficial microbial biomass activity, which, at the same time, can be utilized as a nutritious aquaculture feed, thus lowering the costs of production. Furthermore, BFT permits the installation of integrated multi-trophic aquaculture (IMTA) systems in which the wastes of one organism are utilized as feed by another organism, without a detrimental effect on co-cultured species. This review, therefore, highlights the basics of BFT, factors associated with BFT for the successful production of aquatic species, the significance of this food production system for the sustainable production of economically important aquatic species, its economic aspects, drawbacks, limitations, and recommended management aspects for sustainable aquaculture.

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  • Authors: Yukihiro Tashiro; Kenji Sonomoto; Mohamed Ali Abdel-Rahman;

    Fermentative production of optically pure lactic acid has roused interest among researchers in recent years due to its high potential for applications in a wide range of fields. More specifically, the sharp increase in manufacturing of biodegradable polylactic acid (PLA) materials, green alternatives to petroleum-derived plastics, has significantly increased the global interest in lactic acid production. However, higher production costs have hindered the large-scale application of PLA because of the high price of lactic acid. Therefore, reduction of lactic acid production cost through utilization of inexpensive substrates and improvement of lactic acid production and productivity has become an important goal. Various methods have been employed for enhanced lactic acid production, including several bioprocess techniques facilitated by wild-type and/or engineered microbes. In this review, we will discuss lactic acid producers with relation to their fermentation characteristics and metabolism. Inexpensive fermentative substrates, such as dairy products, food and agro-industrial wastes, glycerol, and algal biomass alternatives to costly pure sugars and food crops are introduced. The operational modes and fermentation methods that have been recently reported to improve lactic acid production in terms of concentrations, yields, and productivities are summarized and compared. High cell density fermentation through immobilization and cell-recycling techniques are also addressed. Finally, advances in recovery processes and concluding remarks on the future outlook of lactic acid production are presented.

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    Authors: Nermin A. El Semary;

    Iron limitation in vast water bodies has been linked to decreased algal productivity, despite different iron-acquiring mechanisms, and the presence of ferritin in many algal species that act as an iron internal reservoir. Therefore, iron fertilization has been proposed to increase algal biomass and photosynthesis. This, in turn, will reduce carbon dioxide in the atmosphere and increase oxygen, thereby decreasing global warming, and achieving ecological balance. In addition, algal proliferation will hopefully lead to enhancement in biodiversity, Biological pump, fish productivity and, subsequently marine food industry. Many climate geoengineering experiments in the form of ocean iron fertilization have been conducted globally in order to achieve such a purpose. However, reservations remain as the outcomes are not as promising as were previously expected. As the temporal and spatial scales of iron fertilization experiments are limited, the effects on fish productivity remain speculative. On the other hand, side effects were also recorded. The main purpose of iron fertilization, for carbon dioxide sequestration and global warming mitigation, still remains to be fully realized and verified. Several improvements and future modifications are suggested, and legal issues are discussed in this review.

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    Authors: Shymaa Ryhan Bashandy; Mohamed Hemida Abd-Alla; Ghada Abd-Elmonsef Mahmoud;

    Abstract Aim This study aims to use fermentation waste of ethanol production (solid and liquid) for riboflavin and recycling of bacterial biomass as biofertilizers to enhance the growth of some oily crop plants. Methods and Results Out of 10 yeast isolates from fresh milk, Clavispora lusitaniae ASU 33 (MN583181) was able to ferment different concentrations of glucose (2.5%, 5%, 7.5%, 10%, 15% and 20%) into ethanol with high efficiency at 10%. Among seven non-Lactobacillus bacterial isolates recovered from cheese samples, two bacterial isolates Bacillus subtlis-SR2 (MT002768) and Novosphingobium panipatense-SR3 (MT002778) were selected for their high riboflavin production. Different media (control medium, fermentation waste medium and a mixture of the fermentation waste medium and control medium [1:1]) were used for riboflavin production. These media were inoculated by a single or mixture of B. subtlis-SR2, N. panipatense-SR3. The addition of the waste medium of ethanol production to the control medium (1:1) had a stimulatory effect on riboflavin production whether inoculated with either a single strain or a mixture of B. subtlis-SR2 and N. panipatense-SR3. A mixture of fermentation waste and control media inoculated with N. panipatense produced a high riboflavin yield in comparison with other media. Inoculation of Zea mays and Ocimum basilicum plants with either the bacterial biomass waste of riboflavin production (B. subtlis or N. panipatense) or a mixture of B. subtlis and N. panipatense) shows a stimulatory effect on the plant growth in comparison with control (uninoculated plants). Conclusions These results demonstrate the possibility of minimizing the cost of riboflavin and biofertilizer manufacturing via interlinking ethanol and riboflavin with the biofertilizer production technology. Significance and Impact of Study This study outlines the methods of evaluating the strength of spent media by applying procedures developed in the vitamin production industries. Furthermore, bacterial biomass waste can act as an environmentally friendly alternative for agrochemicals.

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    Journal of Applied Microbiology
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    Authors: Seyyed Reza Sobhani; Nasrin Omidvar; Zahra Abdollahi; Ayoub Al-Jawaldeh;

    The need for a shift in diet toward a more sustainable one has reached an urgency in certain regions, including Iran, due to more rapid climate change and a higher level of vulnerability. This study was undertaken to identify and summarize available data on changes required in the current Iranian diet to make it more sustainable and the extent to which current policies in the country have addressed such a shift. In this study, PubMed, Scopus, and Web of science, as well as Iranian scientific search engines, including Scientific Information Database and Magiran, were systematically searched from January 1990 to July 2021. A total of 11 studies and policy analyses were included in this study. Based on the findings, moving Iranian diet toward sustainability will require increase in consumption of dairy, fruits, vegetables, cereals, poultry, and legumes and decrease in consumption of bread, rice, pasta, red meat, eggs, fats, sugars, and sweets. There has been a great deal of effort and investment on policies and strategies to decrease the amount of sugar, salt, and fat (specifically trans-fatty acids) in the Iranian diet, which makes it more sustainable healthwise. Several policies and programs have been implemented to tackle non-communicable diseases (NCDs) by reducing access to unhealthy foods, which is in line with health dimension of a sustainable diet. However, there is almost no direct address to ecological aspect of sustainable diet in the food and nutrition policy documents in the ccountry. Development of an enabling environment to a sustainable diet will require policy and actions to improve public awareness, support study to provide evidence and identify possible alternatives, and plan and implement interventions/programs to promote and facilitate healthy and sustainable diets.

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    Frontiers in Nutrition
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    Frontiers in Nutrition
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    Authors: R. Z. Sayyed; S. S. Shaikh; S. J. Wani; Md Tabish Rehman; +3 Authors

    The present study was aimed to evaluate the suitability of agro-wastes and crude vegetable oils for the cost-effective production of poly-β-hydroxybutyrate (PHB), to evaluate growth kinetics and PHB production in Alcaligenes faecalis RZS4 and Pseudomonas sp. RZS1 with these carbon substrates and to study the biodegradation of PHB accumulated by these cultures. Alcaligenes faecalis RZS4 and Pseudomonas sp. RZS1 accumulates higher amounts of PHB corn (79.90% of dry cell mass) and rice straw (66.22% of dry cell mass) medium respectively. The kinetic model suggests that the Pseudomonas sp. RZS1 follows the Monod model more closely than A. faecalis RZS4. Both the cultures degrade their PHB extract under the influence of PHB depolymerase. Corn waste and rice straw appear as the best and cost-effective substrates for the sustainable production of PHB from Alcaligenes faecalis RZS4 and Pseudomonas sp. RZS1. The biopolymer accumulated by these organisms is biodegradable in nature. The agro-wastes and crude vegetable oils are good and low-cost sources of nutrients for the growth and production of PHB and other metabolites. Their use would lower the production cost of PHB and the low-cost production will reduce the sailing price of PHB-based products. This would promote the large-scale commercialization and popularization of PHB as an ecofriendly bioplastic/biopolymer.

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    Molecules
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    Authors: Sang Un Oh; Reda A.I. Abou-Shanab; Byong-Hun Jeon; Nagah M.A. Hassanin; +4 Authors

    La production de biodiesel à partir de microalgues dépend de la biomasse algale et de la teneur en lipides. La production de biomasse et l'accumulation de lipides sont limitées par plusieurs facteurs dans lesquels les nutriments jouent un rôle clé. Nous avons étudié les influences des micronutriments sur la biomasse et la teneur en lipides de Micractinium pusillum GU732425 cultivé en milieu basal gras (BBM). La biomasse sèche moyenne de la souche de microalgues dans le milieu témoin a atteint 0,34 ± 0,01 g /L, tandis que le doublement (2X) des niveaux de concentration en Mn et Cu a augmenté la biomasse sèche à 0,38 ± 0,01 et 0,37 ± 0,02 g /L, respectivement.M. pusillum cultivé en milieu témoin avait une biomasse de 0,82 ± 0,05 g/L et une productivité lipidique de 0,33 ± 0,02 g/L après 17 jours de culture.M. pusillum cultivé en BBM avec 4X Mn ou 4X Cu produisait plus de biomasse (1,25 ± 0,01 ou 1,28 ± 0,04 g pc/L) et de productivité lipidique (0,45±0,04 ou 0,47±0,05g/L), respectivement.M. pusillum cultivé dans différents milieux de croissance avait des compositions d'acides gras comprenant principalement des acides linoléique (49-54%), palmitique (24-29%), linolénique (16-22 %) et les acides oléiques (2-5 %). Ces résultats peuvent être utilisés pour maximiser la production de biomasse et de lipides de microalgues dans des photobioréacteurs conçus de manière optimale. La producción de biodiesel a partir de microalgas depende de la biomasa de algas y el contenido de lípidos. Tanto la producción de biomasa como la acumulación de lípidos están limitadas por varios factores en los que los nutrientes juegan un papel clave. Investigamos las influencias de los micronutrientes en la biomasa y el contenido de lípidos de Micractinium pusillum GU732425 cultivado en medios basales en negrita (BBM). La biomasa seca promedio de la cepa de microalgas en el medio de control alcanzó 0.34 ± 0.01 g /L, mientras que duplicando (2X) los niveles de concentración de Mn y Cu aumentó la biomasa seca a 0.38 ± 0.01 y 0.37 ± 0.02 g /L, respectivamente. M. pusillum cultivado en medio de control tuvo una biomasa de 0.82 ± 0.05 g/L y una productividad lipídica de 0.33 ± 0.02 g/L después de 17 días de cultivo. El alga cultivada en BBM con 4X Mn o 4X Cu produjo más biomasa (1.25 ± 0.01 o 1.28 ± 0.04 g dw/L) y productividad lipídica (0.45±0.04 o 0.47±0.05g/L), respectivamente. M. pusillum cultivado en diferentes medios de crecimiento tuvo composiciones de ácidos grasos que comprenden principalmente linoleico (49-54%), palmítico (24-29%), linolénico (16-22%) y ácidos oleicos (2-5%). Estos resultados se pueden utilizar para maximizar la producción de biomasa y lípidos de microalgas en fotobiorreactores diseñados de manera óptima. Biodiesel production from microalgae depends on the algal biomass and lipid content.Both biomass production and lipid accumulation are limited by several factors in which nutrients play a key role.We investigated the influences of micronutrients on biomass, and lipid content of Micractinium pusillum GU732425 cultivated in bold basal media (BBM).The average dry biomass of microalgal strain in control medium reached 0.34 ± 0.01 g /L, while doubling (2X) the levels of Mn and Cu concentration increased the dry biomass to 0.38 ± 0.01 and 0.37 ± 0.02 g /L, respectively.M. pusillum cultivated in control medium had a biomass of 0.82 ± 0.05 g/L and a lipid productivity of 0.33 ± 0.02 g/L after 17 day cultivation.The alga cultivated in BBM with 4X Mn or 4X Cu produced more biomass (1.25 ± 0.01 or 1.28 ± 0.04 g dw/L) and lipid productivity (0.45±0.04 or 0.47±0.05g/L), respectively.M. pusillum cultivated in different growth media had fatty acid compositions mainly comprising linoleic (49-54%), palmitic (24-29%), linolenic (16-22%), and oleic acids (2-5%).These results can be used to maximize the production of microalgal biomass and lipids in optimally designed photobioreactors. يعتمد إنتاج الديزل الحيوي من الطحالب الدقيقة على الكتلة الحيوية للطحالب ومحتوى الدهون. كل من إنتاج الكتلة الحيوية وتراكم الدهون محدودان بعدة عوامل تلعب فيها العناصر الغذائية دورًا رئيسيًا. لقد حققنا في تأثيرات المغذيات الدقيقة على الكتلة الحيوية، وزاد محتوى الدهون من Micractinium pusillum GU732425 المزروع في الوسائط القاعدية الجريئة (BBM). بلغ متوسط الكتلة الحيوية الجافة لسلالة الطحالب الدقيقة في وسط التحكم 0.34 ± 0.01 جم /لتر، بينما أدى مضاعفة (2X) مستويات تركيز Mn و Cu إلى زيادة الكتلة الحيوية الجافة إلى 0.38 ± 0.01 و 0.37 ± 0.02 جم /لتر، على التوالي. كان لبوسيلوم المزروع في وسط التحكم كتلة حيوية تبلغ 0.82 ± 0.05 جم/لتر وإنتاجية دهنية تبلغ 0.33 ± 0.02 جم/لتر بعد 17 يومًا من الزراعة. الطحالب المزروعة في BBM مع 4X Mn أو 4X Cu أنتجت المزيد من الكتلة الحيوية (1.25 ± 0.01 أو 1.28 ± 0.04 جم dw/L) وإنتاجية دهنية (0.45±0.04 أو 0.47±0.05جم/لتر)، على التوالي. كان لبوسيلوم المزروع في وسائط نمو مختلفة يحتوي على تركيبات الأحماض الدهنية التي تتكون أساسًا من اللينوليك (49-54 ٪)، النخيل (24-29 ٪)، اللينولينيك (16-22 ٪)، وأحماض الأوليك (2-5 ٪). يمكن استخدام هذه النتائج لزيادة إنتاج الكتلة الحيوية للطحالب الدقيقة والدهون في المفاعلات الحيوية الضوئية المصممة على النحو الأمثل.

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    African Journal of Biotechnology
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      African Journal of Biotechnology
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