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Abstract Nitrous oxide (N2O) is an important greenhouse gas and fundamental questions about the capacity of soil microbial communities to act not only as sources, but also as sinks for N2O remains unanswered. We evaluated the capacity of non-denitrifying N2O-reducers to mitigate the production of this greenhouse gas in soil. We showed experimentally that the addition of the non-denitrifying strain Dyadobacter fermentans, which possesses the previously unaccounted N2O reductase NosZII, to 11 different soils significantly reduced N2O production of up to 189% in more than 1/3 of the soils. The magnitude of this effect was significantly influenced by the soil pH and C/N ratio. Overall, our results provide unambiguous evidence that the overlooked non-denitrifying NosZII-type bacteria can contribute to N2O consumption in soil.

Abstract Nitrous oxide (N2O) is an important greenhouse gas and fundamental questions about the capacity of soil microbial communities to act not only as sources, but also as sinks for N2O remains unanswered. We evaluated the capacity of non-denitrifying N2O-reducers to mitigate the production of this greenhouse gas in soil. We showed experimentally that the addition of the non-denitrifying strain Dyadobacter fermentans, which possesses the previously unaccounted N2O reductase NosZII, to 11 different soils significantly reduced N2O production of up to 189% in more than 1/3 of the soils. The magnitude of this effect was significantly influenced by the soil pH and C/N ratio. Overall, our results provide unambiguous evidence that the overlooked non-denitrifying NosZII-type bacteria can contribute to N2O consumption in soil.

It remains unclear how soil microbes respond to labile organic carbon (LOC) inputs and how temperature sensitivity ($Q_{10}$) of soil organic matter (SOM) decomposition is affected by LOC inputs in a short-term. In this study, $^{13}$C-labeled glucose was added to a pristine grassland soil at four temperatures (10, 15, 20, and 25 ◦C), and the immediate utilization of LOC and native SOM by microbes was measured minutely in a short-term. We found that the LOC addition stimulated the native SOM decomposition, and elevated temperature enhanced the intensity of microbial response to LOC addition. The ratio between microbial respiration derived from LOC and native SOM increased with higher temperature, and more LOC for respiration. Additionally, LOC addition increased the $Q_{10}$ of SOM decomposition, and the $Q_{10}$ of LOC decomposition is higher than that of native SOM. Overall, these findings emphasize the important role of temperature and LOC inputs in soil C cycles.

It remains unclear how soil microbes respond to labile organic carbon (LOC) inputs and how temperature sensitivity ($Q_{10}$) of soil organic matter (SOM) decomposition is affected by LOC inputs in a short-term. In this study, $^{13}$C-labeled glucose was added to a pristine grassland soil at four temperatures (10, 15, 20, and 25 ◦C), and the immediate utilization of LOC and native SOM by microbes was measured minutely in a short-term. We found that the LOC addition stimulated the native SOM decomposition, and elevated temperature enhanced the intensity of microbial response to LOC addition. The ratio between microbial respiration derived from LOC and native SOM increased with higher temperature, and more LOC for respiration. Additionally, LOC addition increased the $Q_{10}$ of SOM decomposition, and the $Q_{10}$ of LOC decomposition is higher than that of native SOM. Overall, these findings emphasize the important role of temperature and LOC inputs in soil C cycles.

<p>Global warming is leading to increased temperatures, accentuated evaporation of terrestrial water and increased the atmosphere moisture content, resulting in frequent droughts and heavy precipitation events. It necessary to assess the sensitivity of soil organic carbon (SOC) under storing practices in response to increasing soil moisture, temperature and frequent dry-wet cycles in order to anticipate future soil carbon losses. We evaluated the impact of these climatic events through an incubation experiment on temperate luvisols from conservation agriculture, organic agriculture, organic waste products applications, i.e. biowaste, residual municipal solid waste and farmyard manure composts compared with conventionally managemed soils. The alternative management options all have led to increased SOC stocks. Soil samples were incubated in the lab under different temperatures (20, 28 and 35°C), different moisture conditions (pF1.5; 2.5 and 4.2) and under dry(pF4.2)-wet (pF1.5) cycles. Dry-wet cycles caused CO2 flushes but overall did not stimulate soil carbon mineralization relative to wet controls (pF1.5 and pF2.5). Overall the additional SOC stored under alternative management options was not more sensitive to climate change (temperature, moisture, dry-wet cycles) than the existing SOC.</p>

<p>Global warming is leading to increased temperatures, accentuated evaporation of terrestrial water and increased the atmosphere moisture content, resulting in frequent droughts and heavy precipitation events. It necessary to assess the sensitivity of soil organic carbon (SOC) under storing practices in response to increasing soil moisture, temperature and frequent dry-wet cycles in order to anticipate future soil carbon losses. We evaluated the impact of these climatic events through an incubation experiment on temperate luvisols from conservation agriculture, organic agriculture, organic waste products applications, i.e. biowaste, residual municipal solid waste and farmyard manure composts compared with conventionally managemed soils. The alternative management options all have led to increased SOC stocks. Soil samples were incubated in the lab under different temperatures (20, 28 and 35°C), different moisture conditions (pF1.5; 2.5 and 4.2) and under dry(pF4.2)-wet (pF1.5) cycles. Dry-wet cycles caused CO2 flushes but overall did not stimulate soil carbon mineralization relative to wet controls (pF1.5 and pF2.5). Overall the additional SOC stored under alternative management options was not more sensitive to climate change (temperature, moisture, dry-wet cycles) than the existing SOC.</p>

The severity of drought is predicted to increase across Europe due to climate change. Droughts can substantially impact terrestrial nitrogen (N) cycling and the corresponding microbial communities. Here, we investigated how ammonia-oxidizing bacteria (AOB), archaea (AOA), and complete ammonia oxidizers (comammox) as well as inorganic N pools and N2O fluxes respond to simulated drought under different cropping systems. A rain-out shelter experiment was conducted as part of a long-term field experiment comparing cropping systems that differed mainly in fertilization strategy (organic, mineral, or mixed mineral and organic) and plant protection management (biodynamic versus conventional pesticide use). We found that the effect of drought varied depending on the specific ammonia-oxidizing (AO) groups and the type of cropping system. Drought had the greatest impact on the structure of the AOA community compared to the other AO groups. The abundance of ammonia oxidizers was also affected by drought, with comammox clade B exhibiting the highest sensitivity. Additionally, drought had, overall, a stronger impact on the AO community structure in the biodynamic cropping system than in the mixed and mineral-fertilized conventional systems. The responses of ammonia-oxidizing communities to drought were comparable between bulk soil and rhizosphere. We observed a significant increase in NH4+ and NO3− pools during the drought period, which then decreased after rewetting, indicating a strong resilience. We further found that drought altered the complex relationships between AO communities and mineral N pools, as well as N2O fluxes. These results highlight the importance of agricultural management practices in influencing the response of nitrogen cycling guilds and their processes to drought. Soil Biology and Biochemistry, 201 ISSN:0038-0717 ISSN:1879-3428