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  • Energy Research
  • 13. Climate action
  • 7. Clean energy
  • 2. Zero hunger
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  • Authors: Boulon, Loïc;

    Cette modélisation présente la partie de contrôle d'une pile à combustible à membrane d'échange de protons (PEMFC). L'objectif du modèle de représenter la chaîne de contrôle de la tension de la PEMFC. Ce modèle est basé sur une représentation macroscopique énergétique (REM) de la pile à combustible, puis conduit à une structure dite de contrôle maximal (SCM). La SCM est une inversion étape par étape de la REM (structure de contrôle basée sur un modèle d'inversion). Le processus de conception de la commande est basé sur une définition explicite du problème. Par exemple, les entrées de réglage, les objectifs du système ou les contraintes sont mises en évidence pour organiser la commande. De plus, le SMC montre les endroits où les capteurs sont nécessaires et où les contrôleurs sont requis. Malheureusement, le SCM n'est qu'une structure de contrôle théorique. Par conséquent, une structure réaliste nécessite certaines simplifications, ce qui conduit à une structure de contrôle dite pratique. This modeling presents the control part of a proton exchange membrane fuel cell (PEMFC). The objective of the model is to represent the voltage control chain of the PEMFC. This model is based on an energetic macroscopic representation (EMR) of the fuel cell and then leads to a so-called maximum control structure (MCS). The SCM is a step-by-step inversion of the REM (control structure based on an inversion model). The control design process is based on an explicit problem definition. For example, control inputs, system objectives, or constraints are highlighted to organize the control. In addition, the MSC shows where sensors are needed and where controllers are required. Unfortunately, the SCM is only a theoretical control structure. Therefore, a realistic structure requires some simplifications, which leads to a so-called practical control structure. AdditionnalInformation: Modèle créé lors d'une thèse en génie électrique, par Loïc Boulon (ses recherches portent sur la modélisation et le contrôle des véhicules électriques hybrides, des sources d'énergie et d'énergie et de puissance, et des systèmes de piles à combustible). Il a travaillé en vue de l'obtention du diplôme de doctorat à l'Université de Franche-Comté, Belfort, France, où sa thèse de doctorat a été réalisée en collaboration avec le FEMTO-ST, Belfort, et les Laboratoires L2EP, Lille. Co-encadrement par Daniel Hissel (PU au laboratoire FEMTO-ST), Alain Bouscayrol (PU au laboratoire L2EP) et Marie-Célie Péra (PU au laboratoire FEMTO-ST) AdditionnalInformation: Model created during a thesis in electrical engineering, by Loïc Boulon (his research focuses on the modeling and control of hybrid electric vehicles, energy and power sources, and fuel cell systems). He worked towards his Ph.D. degree at the University of Franche-Comté, Belfort, France, where his dissertation was complet in collaboration with the FEMTO-ST, Belfort, and the L2EP Laboratories, Lille. Co-supervision by Daniel Hissel (PU at FEMTO-ST laboratory), Alain Bouscayrol (PU at L2EP laboratory) and Marie-Célie Péra (PU at FEMTO-ST laboratory) Données computationnelles ou de simulation: - Langage de programmation : Langage Matlab - Plate-forme/OS : Microsoft Windows, Mac OS, Linux,... - Version : MATLAB R2018a - Statut de développement : Actif Simulation or computational data: - Programming language: Matlab language - Platform/OS: Microsoft Windows, Mac OS, Linux,... - Version: MATLAB R2018a - Development status: Active Project: Thèse de doctorat en génie électrique - Loïc Boulon, Contrat doctoral (Region Bourgogne Franche-Comté) Label: PHE (Plateforme Hydrogène-Energie) Audience: Research

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  • Authors: Morando, Simon; Pahon, Élodie; Pahon, Élodie;

    Le projet PHM-PAC vise principalement le développement de méthodes intelligentes de détection, diagnostic et pronostic des défaillances de piles à combustibles. Ce projet doit permettre l'optimisation des performances des systèmes pile à combustible ainsi qu'une meilleure compréhension des phénomènes électrochimiques en affectant la durabilité. The PHM-PAC project is mainly aimed at developing intelligent methods for the detection, diagnosis and prognosis of fuel cell failures. This project should allow the optimization of the performance of fuel cell systems as well as a better understanding of the electrochemical phenomena affecting the durability. Données expérimentales: 2 800 heures de fonctionnement à charge constante sur une pile à combustible à membrane échangeuse de protons d'une puissance de 500W Experimental data: 2,800 hours of operation at constant load on a 500W proton exchange membrane fuel cell Project: PHM PAC, Région Bourgogne Franche-Comté (Region Bourgogne Franche-Comté) Audience: University: master, Research, University: licence Label: PHE (Plateforme Hydrogène-Energie) Label: Plateforme UBFC (Plateforme UBFC) UpdatePeriodicity: no update

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  • image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
    Authors: Heral, Maurice; Razet, Daniel; Deslous-paoli, Jean-marc; Berthome, Jean-paul; +1 Authors

    Les grandes caractéristiques hydrobiologiques du bassin de Marennes-Oléron sont définies par quatre campagnes saisonnières de 120 prélèvements chacune effectuée-, en 15 stations en demi-cycle de marée. Les salinités y fluctuent de 10 '/rr. et ce secteur peut donc être assimilé à un vaste estuaire contrôlé principalement par les apports fluviaux de la Charente. La turbidité reste élevée souvent supérieure à 100 mg.I"', les teneurs en oxygène dissous montrent un déficit en amont de la Charente et de la Seudre de 4 mg.L1 tandis que les sels nutritifs sont apportés par la Charente pour les nitrates, phosphates et silicates. Ces derniers suivent les courbes de dilution théorique excepté lors des poussées phytoplanctoniques et lors des crues hivernales. Lors de ces crues, les nitrates semblent soit apportés par la Gironde, soit dus à une minéralisation organique. Au même moment, les phosphates pourraient être absorbés sur les particules et être piégés dans les sédiments. Les biomasses phytoplanctoniques exprimées par les teneurs en chlorophylle a sont supésieures à 30 f^-g.1 ' en amont des estuaires au printemps avec des teneurs en phéopigmenls pouvants dépasser 200 IUJ.1 . par contre, dans le bassin, les teneurs ne dépassent guère 10 fig.l ''. Les concentrations en ATP montrent que peu de phytoplancton est physiologiquement actif en particulier dans les estuaires, tandis que les dosages de protéines permettent de quantifier la part des apports allochtone - par rapport à la production autochtone des estuaires. Line analyse factorielle des correspondances définit le schéma général hydrobiologique du bassin estuarien en précisant que 'e rôle de la Charente ne se limite pas à l'apport en sels minéraux azotés et phosphatés, mais aussi à l'apport d'une biomasse phytoplanctonique d'eau douce en voie de dégénérescence. Les auteurs proposent qu'une nouvelle stratégie d'échantillonnage, mieux adaptée aux objectifs recherchés, soit suivie ultérieurement en assurant des prélèvements en cycle de marée mais avec une périodicité la plus élevée possible. Four seasonal compaigns providing a sampling of 15 stations each followed during half a tide cycle, i.e. 120 water samples permit the definition of the main hydrobiological features of Marennes-Oleron Bay: salinity and turbidity, phytoplanktonic biomasses, ATP concentrations. The authors suggest that a new way of adapted sampling to their objectives, will work out in the future by sampling during tidal cycles but as frequently as possible.

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    Authors: Gohin, Alexandre; Gohin, Alexandre;

    [Paper in French] The European public policy in favor of the biodiesel consumption is highly debated. Available estimates of the induced land use changes conclude that this policy is inefficient to reduce emissions of GreenHouse Gas. We show that the crop yield evolutions in these estimates are significantly lower than the observed and expected evolutions. This difference is directly related to biased calibration choice of behavioral parameters. We show using the GTAP-BIO framework that a consistent calibration of these parameters leads to a strong reduction (by around 80% in the long run) of the land use changes and induced emissions.

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    External research report . 2013
    License: CC BY SA
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    https://dx.doi.org/10.22004/ag...
    Other literature type . 2013
    Data sources: Datacite
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      https://dx.doi.org/10.22004/ag...
      Other literature type . 2013
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    Authors: Saulnier, Jean Baptiste;

    This work focuses on the refinement of sea-states spectral characterisation in the frame of the development of wave energy converters (WECs). Until now, this characterisation has been limited to the consideration of basic global parameters such as the significant wave height and mean wave period. The present work namely underlines - thanks to simplified numerical models - that such a description is not sufficient to account for the performance of WECs, for they still exhibit a large variability in terms of mean production while wave energy and period are fixed. It is therefore needed to refine this description by introducing new wave characteristics, such as the wave groupiness, which is itself related to the sea-state's spectral bandwidth. The classical - spectral - approaches, allowing a statistical analysis of wave groups along with other works in the literature, permit to draw a set of relevant spectral parameters. The sensitivity of simplified offshore WEC models (linear in the frequency domain) to these parameters is then observed. The question of deterministic mean power estimation - namely in the case of non-linear models - is also raised, for it is also related somehow to the bandwidth of the wave field. A sea-state is actually made of a superimposition of various wave systems (as swells and wind-sea). It is then possible to refine even more its description, namely through the multi-system approach. The latter enables indeed to track in time and space the wave systems independently of each other. An algorithm for the automatic spatiotemporal analysis is proposed and illustrated to this end. Lastly, the influence of such description refinements (wave groupiness and multi-system approach) is observed at several locations (Portugal, North Sea, California) by considering various WEC models (linear axi-symmetric heaving devices, 3D SEAREV with non-linear hydrodynamics): in all cases, it is shown that both extended descriptions allow for a better characterisation of the mean WEC performance at both short and long term. Ces travaux portent sur la caractérisation spectrale des états de mer dans le cadre du développement de systèmes récupérateurs de l'énergie des vagues (SREVs). Jusqu'alors, cette caractérisation s'est limitée à des paramètres globaux basiques tels que la hauteur significative et la période moyenne des vagues. Les présents travaux soulignent notamment, à l'aide de modèles simplifiés, que cette description est insuffisante car un SREV montre encore une grande variabilité en termes de production moyenne, ces deux paramètres étant fixés par ailleurs. On s'intéresse donc à raffiner cette description en considérant des caractéristiques de vagues nouvelles, comme le groupement de vagues, lui-même lié à la largeur de bande de l'état de mer. Les approches classiques - spectrales - sur l'étude descriptive des groupes de vagues et la littérature permettent de dégager un certain nombre de paramètres spectraux. On observe ensuite la sensibilité de modèles de SREVs offshore simplifiés (modèles fréquentiels linéaires) à ces paramètres de largeur globale. La question de l'estimation déterministe de performance moyenne (dans le cas de convertisseurs non-linéaires) est également abordée, étant elle-même liée à cette caractéristique. Un état de mer étant constitué de plusieurs systèmes de vagues (houles, mer de vent), il est encore possible de raffiner sa description grâce à une approche multisystème. Celle-ci permet en outre de suivre ces systèmes indépendamment dans le temps et l'espace. Un algorithme d'analyse spatio-temporelle est proposé et illustré à cet égard. Enfin, l'influence de ces raffinements est observée en différents sites (Portugal, Mer du Nord, Californie) sur des cas de SREVs divers (systèmes axi-symétriques linéaires, SEAREV 3D d'hydrodynamique non-linéaire), et montre que dans tous les cas, le raffinement apporté permet de mieux caractériser la production à court et long terme de ces dispositifs.

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    Authors: Maestrini, S; Rochet, M; Legendre, L; Demers, S;

    Nutrient analyses were done in melted ice, frazil and sea water of the upper part of the water column, where as major constitutive elements of micro-algae were analysed. Measurements of nutrient uptake were carried out with frazil algae incubated at -1.5 degree C in a field incubator. Differential-enrichment bioassays and mean N/P ratio (5.9) demonstrated that actual growth of the cultures was nutrient-limited. Nitrogen governed the algal yield when illumination and grazing allowed the microalgae to grow. It concluded that the epontic dynamics is not only controlled from above, by the seasonal (climatic) changes in light intensity as generally assumed, but also from below, by the short-term (hydrodynamic) events of vertical mixing that replenish the ice-water interface in nutrients.

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    Authors: Koutou, Mahamoudou; Sangaré, Mamadou; Havard, Michel; Toillier, Aurélie; +3 Authors

    Income source and accompaniment needs of family farms in the cotton growing area of West Burkina Faso Description of the subject. In the western cotton growing area of Burkina Faso, the variability and the diversity of the income levels of family farms (EAFs) mean that these farms require suitable agronomic accompaniment. Objectives. This article gives an account of this diversity and the need for accompaniment according to the type of identified EAF.Method. The data were collected by the active method of participative research and by investigation of the practices of 120 farms in the commune of Koumbia. Principal component analyses with economic indicators of the EAF were applied, followed by an ascending hierarchical clustering. The structural characteristics, the agro-pastoral practices of production, and the perceptions of the EAF regarding the constraints and appropriateness of agricultural production were compared by variance analysis. Results. The results showed three types of farm: farms with a low-income culture (farmers with few animals), those with a high income culture (farmers with a large number of animals) and those deriving income from breeding (stockbreeders). Taking into account the structure of the farms, their production practices, their technico-economic performance and their appropriateness of agricultural production, the form of sustainable accompaniment needed may be identified in relation to several common or specific innovations of the identified farm types. Accompaniments in the case of farm stock management, and good practice in the management of soil fertility and of animals were common to the three types of EAF. The fodder culture, fattening on pasture and the method of dairy production were specific to the farmers with a large number of animals and to the stockbreeders. The improvement of the food value of culture residues and of some aspects of the maintenance of oxen were more appropriate to the farmers with few animals. Conclusions. In order to succeed, the process of accompaniment must take into account both these specificities and the concept of sustainability. Description du sujet. Dans la zone cotonnière ouest du Burkina Faso, la variabilité et la diversité des revenus des exploitations agricoles familiales (EAF) demandent pour ces dernières des accompagnements appropriés. Objectifs. Cet article rend compte de cette diversité et des besoins d’accompagnement selon les types d’EAF identifiés.Méthode. Les données ont été collectées par la méthode active de recherche participative et par enquête auprès d’un échantillon raisonné de 120 exploitations dans la commune de Koumbia. Une analyse en composante principale avec des indicateurs économiques de l’EAF suivie d’une classification ascendante hiérarchique ont été appliquées. Les caractéristiques structurelles, les pratiques de production agro-pastorales, les perceptions des chefs d’EAF sur les contraintes et opportunités de production agropastorale ont été comparées par une analyse de variance.Résultats. Les résultats montrent trois types d’exploitations : les exploitations à faible revenu culture (agriculteurs), à fort revenu culture (agro-éleveurs) et à revenu élevage (éleveurs). À la lumière de leurs structures, pratiques de production, performances technico-économiques et opportunités de production agricole, leur accompagnement peut porter sur plusieurs innovations durables communes ou spécifiques des types identifiés. Des accompagnements sur la gestion des ressources de l’exploitation, les bonnes pratiques de gestion de la fertilité des sols et des animaux sont communs aux trois types d’EAF. Les cultures fourragères, l’embouche et la production laitière sont spécifiques aux agro-éleveurs et éleveurs. L’amélioration de la valeur nutritive des résidus de cultures et l’entretien des bœufs de trait conviennent plus aux agriculteurs.Conclusions. Pour réussir, le processus d’accompagnement doit prendre en compte ces spécificités et la notion de durabilité.

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    Biotechnologie, Agronomie, Société et Environnement
    Article . 2016 . Peer-reviewed
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      Biotechnologie, Agronomie, Société et Environnement
      Article . 2016 . Peer-reviewed
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  • Authors: Vichard, Loic;

    Ces modélisations, sont associées au projet AsDeCoEUR (Analyses De Composants Energétiques en Usage Réel). Le projet souhaite s’appuyer sur une démarche menée autour d’un travail de thèse visant à comprendre le comportement dynamique, d’étudier le vieillissement et d’estimer l’état de santé des composants énergétiques, batterie et pile à combustible en usage réel. Cette démarche est basée sur l’analyse des données enregistrées sur ces composants dans le projet Mobypost (flotte de 10 véhicules Hydrogènes). La mise en œuvre des compétences et des outils d’analyse numériques développés notamment pour les batteries par le laboratoire Ampère et l’IFSTTAR vise à comprendre et à reproduire les phénomènes de dégradation des composants étudiés. Ces modèles permettent de contribuer à l’étude du vieillissement et d’estimer l’état de santé en usage réel des batteries et des piles à combustible. These models are associated with the project AsDeCoEUR (Analyses De Composants Energétiques en Usage Réel). The project is based on a thesis work aiming at understanding the dynamic behavior, to study the aging and to estimate the health status of the battery and fuel cell energy components in real use. This approach is based on the analysis of data recorded on these components in the Mobypost project (fleet of 10 hydrogen vehicles). The implementation of skills and numerical analysis tools developed in particular for batteries by the Ampère laboratory and IFSTTAR aims to understand and reproduce the degradation phenomena of the components studied. These models contribute to the study of ageing and estimate the state of health of batteries and fuel cells in real use. AdditionnalInformation: Model produced within the framework of Loic Vichard's thesis in electrical engineering (Contribution to the study of the aging of battery and fuel cell components in real use), under the supervision of Daniel Hissel (PU at the FEMTO-ST laboratory), Alexandre Ravey (MCF at UTBM) and Pascal Venet (PU at the Claude Bernard Lyon 1 University). AdditionnalInformation: Modèle produit dans le cadre de la thèse en génie électrique de Loic Vichard (Contribution à l'étude du vieillissement des composantes batteries et pile à combustible en usage réel), sous la direction de Daniel Hissel (PU au laboratoire FEMTO-ST), Alexandre Ravey (MCF à l'UTBM) et Pascal Venet (PU à l'université Claude Bernard Lyon 1). Données computationnelles ou de simulation: - Langage de programmation : Langage Matlab et Python - Plate-forme/OS : Microsoft Windows, Mac OS, Linux,... - Version : MATLAB R2018a - Statut de développement : Actif Simulation or computational data: - Programming language: Matlab language et Python language - Platform/OS: Microsoft Windows, Mac OS, Linux,... - Version: MATLAB R2018a - Development status: Active Project: Thèse de doctorat en génie électrique - Loic Vichard, Contrat doctoral - Projet AsDeCoEUR (Region Bourgogne Franche-Comté) Label: PHE (Plateforme Hydrogène-Energie) UpdatePeriodicity: no update Audience: Research

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    Authors: Brunel, Thomas;

    Le recrutement - nombre de jeunes individus issus de la reproduction qui contribuent annuellement au renouvellement des stocks de poissons exploités - est un facteur clé de la dynamique de ces stocks. La forte variabilité du recrutement de la plupart des populations est liée en grande partie à l'influence des facteurs de l'environnement sur la survie lors des premiers stades de développement. La compréhension du déterminisme de cette variabilité est primordiale pour une gestion efficace des stocks. Cette thèse avait pour objectif de généraliser un certain nombre de points concernant l'influence de l'environnement sur le recrutement, par l'utilisation d'une approche macroécologique portant sur l'ensemble des populations exploitées de l'Atlantique Nord-Est. Le premier chapitre de la thèse répertorie les différentes sources de données de recrutement disponibles, évalue leur cohérence et leur fiabilité. Les estimations du recrutement issues des modèles d'analyse de cohortes, concernant plus de 60 populations de 18 espèces, sont finalement retenues pour l'analyse de la variabilité du recrutement. Le second chapitre présente les concepts et les méthodes d'analyse de la variabilité du recrutement. La synthèse des grandes hypothèses du déterminisme du recrutement permet de préciser les influences respectives des facteurs environnementaux et des autres facteurs sur la variabilité du recrutement. Les relations stock/recrutement sont ensuite présentées, en partant du cas concret des populations considérées dans cette étude. Le chapitre se termine par la présentation des différentes voies d'étude de la variabilité du recrutement et la justification du choix d'une approche macroécologique. Le troisième chapitre analyse les corrélations des variations du recrutement entre les populations et met en évidence des ensembles de populations dont les variations du recrutement ont été synchrones. Ces synchronismes représentent des communautés de réponse du recrutement des populations à l'influence des facteurs environnementaux et témoignent donc du contrôle environnemental des variations du recrutement. Le quatrième chapitre analyse les différences de variabilité du recrutement entre les populations. Il est notamment montré que les populations situées près des limites Nord et Sud des aires de répartition géographique des espèces présentent une variabilité du recrutement plus forte que celles situées au centre de ces aires. Cette relation est cohérente avec les théories de la macroécologie, selon lesquelles les populations en limite de distribution seraient affectées de façon plus marquée par les fluctuations des conditions environnementales qu'elles rencontrent. Le cinquième chapitre examine la correspondance entre les variations à long terme du recrutement et les changements récents du climat dans l'Atlantique Nord. La tendance dominante du recrutement de 40 populations durant les trente dernières années est fortement corrélée au signal dominant des variations de la température de surface du Nord-Est Atlantique, lui-même corrélé à la cinétique globale du réchauffement climatique. Ce chapitre suggère également que la réponse des populations à ce réchauffement pourrait dépendre de leur position dans les aires de répartition des espèces. Recruitment - the number of young fishes that integrate each year the exploitable stock¿ has a central importance for stock dynamics. The high temporal variability in recruitment is a result of the environmental influence on survival during early life stages. Understanding the determinism of the influence of environment on recruitment remains one of the most important question in fisheries ecology. The present work aimed at answering some general questions about the influence of environment on fish recruitment using a macroecological approach. The study focused on the exploited fish populations of the Northeastern Atlantic. The first chapter of the thesis makes a review of the different methods used to estimate recruitment, compares recruitment estimates from these different methods, and discusses their reliability. Recruitment estimates from cohort analysis models, concerning more than 60 populations of 18 species, are finally retained to study recruitment variability. The second chapter presents the main concepts and the different approaches to the study of recruitment variability. A review of the major hypotheses concerning recruitment determinism is first made in order to detail the respective influence of environmental factors and of other factors on recruitment variability. The link between stock size and recruitment is then presented, based on the analysis of the data concerning the populations considered in this work. The chapter ends with a presentation of the different approaches used to study recruitment variability, and the justification of the choice of a macroecological approach. The third chapter analyses correlations in recruitment variations among Northeast Atlantic fish stocks, and shows the existence of groups of populations with synchronous recruitment variations. Such synchronies in recruitment correspond to similar responses of recruitment to environmental factors and hence indicate an environmental control of recruitment variations. The fourth chapter analyses the differences in recruitment variability among populations. Recruitment variability for populations living near the borders of species distribution ranges is higher than for populations living at the centre of these ranges. According to macroecological theories this pattern of recruitment variability within species geographical ranges suggests that populations on the border of species range are more strongly influenced by environmental variability than populations at the centre of the range. The fifth chapter investigates coherence between long term trends in recruitment and the changes in the North Atlantic climate during the last three decades. The dominant trend of recruitment variations for 40 populations, extracted by a principal component analysis, is strongly negatively correlated with the dominant signal in sea surface temperature in the North-East Atlantic, which is highly correlated to the global increase in temperature related to global warming. The chapter also suggests that the impact of the global warming on fish recruitment may depend on population position in species range.

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    Authors: Boullet, Antoine;

    When it comes to sustainable development, n-butanol production by biological means represents an environmentally friendly alternative compared to petrochemical means. Some organisms are able to produce butanol naturally, such as the Clostridiae family, which are Gram-positive bacteria, growing in strict anaerobic conditions. They produce butanol in mixture with acetone and ethanol, which reduce the production yield of butanol and increase the purification costs. In 2015, the PEEP team of TBI built a genetically modified strain of E. coli, expressing the complete conversion pathway from pyruvate to butanol from C. acetobutylicum. This strain’s metabolism was built so that the growth and glucose consumption speeds are coupled with the butanol production speed. In strict anaerobic growth in batch culture, in a chemically defined medium based on glucose and supplemented with yeast extracts (YE) and nitrate (NO3-), this strain produces 3,3 g/L of n-butanol, with a yield of 0,23 g butanol/g glucose, among other minority coproducts (succinate, lactate, acetate, butyrate). These coproducts lower the butanol production yield, and remain unexpected, because the corresponding metabolic pathways were deleted. The goal of this PhD project consists in identifying the metabolic pathways involved in the coproducts production and improving the understanding of the strain, in order to optimize it, improve the butanol/glucose yield, and simplify the culture medium. Several aspects are considered: expressing ferredoxin NAD(P)+ oxidoreductases (FNOR) from organisms other than C. acetobutylicum, in order to combine the reoxidation of the reduced ferredoxin to the generation of ATP; ii) inactivating the metabolic pathways from E. coli which may be involved in the coproducts synthesis; iii) performing an evolutionary in-vivo adaptation of the strain, in order to improve its performances and increase its tolerance to butanol in a chemically defined medium supplemented with YE and NO3-, and then iv) performing an evolutionary adaptation of the Butanol strain to allow its growth in the chemically defined medium without any supplementation. These strategies lead to i) the construction of a new E. coli strain, with its anaerobic growth depending on the functionality of an FNOR: the FNOR from C. acetobutylicum, the Rnf complex from C. ljungdahlii and the FNOR from C. tepidum were assessed. This work lead to the selection of a mutant of the FNOR from C. tepidum, which has a ferredoxin NAD+ reductase activity 2,2 times higher than the native enzyme. This mutant FNOR was then expressed in the E. coli strain producing butanol, as a replacement of C. acetobutylicum’s. The characterization of its phenotype demonstrated its capacity to produce 3 g/L of butanol, with a yield of 0,26 g/g; ii) the deletion of the genes zwf and mdh in the Butanol strain also lead to an improvement of the production in butanol up to 6 g/L , and of the yield up to 0,33 g/g; iii) the in-vivo evolution of the Butanol strain, in continuous culture (chemostat) with regulated pH, in the medium supplemented with YE and Ni, lead to the selection of an evolved strain producing up to 11 g/L of butanol, and a yield of 0,34 g/g. The complete sequencing of the genomes of the clones isolated from this population lead to the identification of mutations in two genes, yqhC and lptG, potentially involved in the strain’s performances; iv) the in-vivo adaptation of the Butanol strain in the medium without any supplementation lead to the selection of a evolved clones producing 2,5 g/L of butanol, and a yield of 0,28 g/g in batch culture. To our knowledge, the highest production of butanol observed coupled with anaerobic growth in E. coli does not exceed 0,6g/L, in a chemically defined medium base on glucose without any supplementation.; Dans le cadre du développement durable, la production de n-butanol par voie biologique est une alternative écologique par rapport à sa synthèse par voie pétrochimique. Il existe des microorganismes naturellement capables de produire du butanol, tels que les Clostridiae, bactéries Gram positif, ayant une croissance strictement anaérobie. Ils produisent du butanol au travers d’une fermentation produisant un mélange d’acétone, de butanol et d’éthanol. La production de butanol en mélange avec d’autres molécules diminue le rendement de production en butanol et augmente le coût de purification. En 2015, l’équipe PEEP de TBI a construit une souche d’E. coli génétiquement modifiée exprimant la voie complète de conversion du pyruvate en n-butanol de C. acetobutylicum. Le métabolisme de cette souche a été conçu pour que les vitesses de croissance et de consommation du glucose soient couplées à la vitesse de production du butanol. En croissance en anaérobiose stricte en culture discontinue dans un milieu chimiquement défini à base de glucose supplémenté en extrait de levure (YE) et nitrate (NO3-), cette souche produit 3,3 g/L de butanol à un rendement de 0,23 g butanol /g glucose, en présence de coproduits minoritaires (succinate, lactate, acétate, butyrate). Ces coproduits diminuent le rendement de production en butanol et restent inattendus car les voies métaboliques correspondantes ont été supprimées. L’objectif de ce projet de thèse est d’identifier les voies métaboliques impliquées dans la production de ces coproduits et d’améliorer la compréhension du métabolisme de cette souche, afin de l’optimiser, d’augmenter le rendement butanol/glucose, et de simplifier le milieu de culture. Plusieurs pistes ont été envisagées : i) Expression de ferrédoxines NAD(P)+ oxydoréductases (FNOR) issues d’organismes autres que C. acetobutylicum dans l’objectif de coupler l’étape de réoxydation de la ferrédoxine réduite à la production d’ATP ; ii) Inactivation des voies métaboliques d’E. coli susceptibles d’être impliquées dans la synthèse des coproduits ; iii) Evolution adaptative in vivo de la souche, afin d’améliorer ses performances et accroitre sa tolérance au butanol dans le milieu supplémenté avec YE et NO3-, puis iv) Evolution adaptative in vivo de la souche dans le milieu sans supplémentation. Ces stratégies ont conduit à i) La construction d’une nouvelle souche d’E. coli dont la croissance anaérobie dépend de la fonctionnalité d’une FNOR : la FNOR de C. acetobutylicum, le complexe Rnf de C. ljungdahlii et la FNOR de C. tepidum ont été évaluées. Cette approche a conduit à la sélection d’un mutant de la FNOR de C. tepidum, ayant une activité ferrédoxine NAD+ réductase 2,2 fois supérieure à celle de l’enzyme native. Cette FNOR mutante a ensuite été exprimée dans la souche d’E. coli Butanol en remplacement de la FNOR de C. acetobutylicum. La caractérisation de son phénotype a montré sa capacité à produire 3 g/L de butanol à un rendement de 0,26 g/g ; ii) La délétion des gènes zwf et mdh dans la souche Butanol a permis d’augmenter le titre en butanol produit à 6 g/L et le rendement à 0,33 g/g ; iii) L’évolution adaptative in-vivo de la souche d’E. coli Butanol en culture continue, dans le milieu supplémenté avec YE et Ni, a conduit à la sélection d’une population évoluée produisant 11 g/L de butanol à un rendement de 0,34 g/g. Le séquençage complet des génomes de clones isolés à partir de cette population a conduit à l’identification de mutations dans deux gènes, yqhC et lptG, potentiellement impliquées dans les performances de la souche ; iv) L’évolution adaptative in-vivo de la souche Butanol dans le milieu sans supplémentation a conduit à la sélection de clones évolués produisant 2,5 g/L de butanol à un rendement de 0,28 g/g en culture discontinue. A notre connaissance, la production maximale de butanol reportée, couplée à la croissance anaérobie chez E. coli, ne dépasse pas 0,6 g/L, en milieu chimiquement défini à base uniquement de glucose.

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  • Authors: Boulon, Loïc;

    Cette modélisation présente la partie de contrôle d'une pile à combustible à membrane d'échange de protons (PEMFC). L'objectif du modèle de représenter la chaîne de contrôle de la tension de la PEMFC. Ce modèle est basé sur une représentation macroscopique énergétique (REM) de la pile à combustible, puis conduit à une structure dite de contrôle maximal (SCM). La SCM est une inversion étape par étape de la REM (structure de contrôle basée sur un modèle d'inversion). Le processus de conception de la commande est basé sur une définition explicite du problème. Par exemple, les entrées de réglage, les objectifs du système ou les contraintes sont mises en évidence pour organiser la commande. De plus, le SMC montre les endroits où les capteurs sont nécessaires et où les contrôleurs sont requis. Malheureusement, le SCM n'est qu'une structure de contrôle théorique. Par conséquent, une structure réaliste nécessite certaines simplifications, ce qui conduit à une structure de contrôle dite pratique. This modeling presents the control part of a proton exchange membrane fuel cell (PEMFC). The objective of the model is to represent the voltage control chain of the PEMFC. This model is based on an energetic macroscopic representation (EMR) of the fuel cell and then leads to a so-called maximum control structure (MCS). The SCM is a step-by-step inversion of the REM (control structure based on an inversion model). The control design process is based on an explicit problem definition. For example, control inputs, system objectives, or constraints are highlighted to organize the control. In addition, the MSC shows where sensors are needed and where controllers are required. Unfortunately, the SCM is only a theoretical control structure. Therefore, a realistic structure requires some simplifications, which leads to a so-called practical control structure. AdditionnalInformation: Modèle créé lors d'une thèse en génie électrique, par Loïc Boulon (ses recherches portent sur la modélisation et le contrôle des véhicules électriques hybrides, des sources d'énergie et d'énergie et de puissance, et des systèmes de piles à combustible). Il a travaillé en vue de l'obtention du diplôme de doctorat à l'Université de Franche-Comté, Belfort, France, où sa thèse de doctorat a été réalisée en collaboration avec le FEMTO-ST, Belfort, et les Laboratoires L2EP, Lille. Co-encadrement par Daniel Hissel (PU au laboratoire FEMTO-ST), Alain Bouscayrol (PU au laboratoire L2EP) et Marie-Célie Péra (PU au laboratoire FEMTO-ST) AdditionnalInformation: Model created during a thesis in electrical engineering, by Loïc Boulon (his research focuses on the modeling and control of hybrid electric vehicles, energy and power sources, and fuel cell systems). He worked towards his Ph.D. degree at the University of Franche-Comté, Belfort, France, where his dissertation was complet in collaboration with the FEMTO-ST, Belfort, and the L2EP Laboratories, Lille. Co-supervision by Daniel Hissel (PU at FEMTO-ST laboratory), Alain Bouscayrol (PU at L2EP laboratory) and Marie-Célie Péra (PU at FEMTO-ST laboratory) Données computationnelles ou de simulation: - Langage de programmation : Langage Matlab - Plate-forme/OS : Microsoft Windows, Mac OS, Linux,... - Version : MATLAB R2018a - Statut de développement : Actif Simulation or computational data: - Programming language: Matlab language - Platform/OS: Microsoft Windows, Mac OS, Linux,... - Version: MATLAB R2018a - Development status: Active Project: Thèse de doctorat en génie électrique - Loïc Boulon, Contrat doctoral (Region Bourgogne Franche-Comté) Label: PHE (Plateforme Hydrogène-Energie) Audience: Research

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  • Authors: Morando, Simon; Pahon, Élodie; Pahon, Élodie;

    Le projet PHM-PAC vise principalement le développement de méthodes intelligentes de détection, diagnostic et pronostic des défaillances de piles à combustibles. Ce projet doit permettre l'optimisation des performances des systèmes pile à combustible ainsi qu'une meilleure compréhension des phénomènes électrochimiques en affectant la durabilité. The PHM-PAC project is mainly aimed at developing intelligent methods for the detection, diagnosis and prognosis of fuel cell failures. This project should allow the optimization of the performance of fuel cell systems as well as a better understanding of the electrochemical phenomena affecting the durability. Données expérimentales: 2 800 heures de fonctionnement à charge constante sur une pile à combustible à membrane échangeuse de protons d'une puissance de 500W Experimental data: 2,800 hours of operation at constant load on a 500W proton exchange membrane fuel cell Project: PHM PAC, Région Bourgogne Franche-Comté (Region Bourgogne Franche-Comté) Audience: University: master, Research, University: licence Label: PHE (Plateforme Hydrogène-Energie) Label: Plateforme UBFC (Plateforme UBFC) UpdatePeriodicity: no update

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    Authors: Heral, Maurice; Razet, Daniel; Deslous-paoli, Jean-marc; Berthome, Jean-paul; +1 Authors

    Les grandes caractéristiques hydrobiologiques du bassin de Marennes-Oléron sont définies par quatre campagnes saisonnières de 120 prélèvements chacune effectuée-, en 15 stations en demi-cycle de marée. Les salinités y fluctuent de 10 '/rr. et ce secteur peut donc être assimilé à un vaste estuaire contrôlé principalement par les apports fluviaux de la Charente. La turbidité reste élevée souvent supérieure à 100 mg.I"', les teneurs en oxygène dissous montrent un déficit en amont de la Charente et de la Seudre de 4 mg.L1 tandis que les sels nutritifs sont apportés par la Charente pour les nitrates, phosphates et silicates. Ces derniers suivent les courbes de dilution théorique excepté lors des poussées phytoplanctoniques et lors des crues hivernales. Lors de ces crues, les nitrates semblent soit apportés par la Gironde, soit dus à une minéralisation organique. Au même moment, les phosphates pourraient être absorbés sur les particules et être piégés dans les sédiments. Les biomasses phytoplanctoniques exprimées par les teneurs en chlorophylle a sont supésieures à 30 f^-g.1 ' en amont des estuaires au printemps avec des teneurs en phéopigmenls pouvants dépasser 200 IUJ.1 . par contre, dans le bassin, les teneurs ne dépassent guère 10 fig.l ''. Les concentrations en ATP montrent que peu de phytoplancton est physiologiquement actif en particulier dans les estuaires, tandis que les dosages de protéines permettent de quantifier la part des apports allochtone - par rapport à la production autochtone des estuaires. Line analyse factorielle des correspondances définit le schéma général hydrobiologique du bassin estuarien en précisant que 'e rôle de la Charente ne se limite pas à l'apport en sels minéraux azotés et phosphatés, mais aussi à l'apport d'une biomasse phytoplanctonique d'eau douce en voie de dégénérescence. Les auteurs proposent qu'une nouvelle stratégie d'échantillonnage, mieux adaptée aux objectifs recherchés, soit suivie ultérieurement en assurant des prélèvements en cycle de marée mais avec une périodicité la plus élevée possible. Four seasonal compaigns providing a sampling of 15 stations each followed during half a tide cycle, i.e. 120 water samples permit the definition of the main hydrobiological features of Marennes-Oleron Bay: salinity and turbidity, phytoplanktonic biomasses, ATP concentrations. The authors suggest that a new way of adapted sampling to their objectives, will work out in the future by sampling during tidal cycles but as frequently as possible.

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    Authors: Gohin, Alexandre; Gohin, Alexandre;

    [Paper in French] The European public policy in favor of the biodiesel consumption is highly debated. Available estimates of the induced land use changes conclude that this policy is inefficient to reduce emissions of GreenHouse Gas. We show that the crop yield evolutions in these estimates are significantly lower than the observed and expected evolutions. This difference is directly related to biased calibration choice of behavioral parameters. We show using the GTAP-BIO framework that a consistent calibration of these parameters leads to a strong reduction (by around 80% in the long run) of the land use changes and induced emissions.

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    External research report . 2013
    License: CC BY SA
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    https://dx.doi.org/10.22004/ag...
    Other literature type . 2013
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      https://dx.doi.org/10.22004/ag...
      Other literature type . 2013
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    Authors: Saulnier, Jean Baptiste;

    This work focuses on the refinement of sea-states spectral characterisation in the frame of the development of wave energy converters (WECs). Until now, this characterisation has been limited to the consideration of basic global parameters such as the significant wave height and mean wave period. The present work namely underlines - thanks to simplified numerical models - that such a description is not sufficient to account for the performance of WECs, for they still exhibit a large variability in terms of mean production while wave energy and period are fixed. It is therefore needed to refine this description by introducing new wave characteristics, such as the wave groupiness, which is itself related to the sea-state's spectral bandwidth. The classical - spectral - approaches, allowing a statistical analysis of wave groups along with other works in the literature, permit to draw a set of relevant spectral parameters. The sensitivity of simplified offshore WEC models (linear in the frequency domain) to these parameters is then observed. The question of deterministic mean power estimation - namely in the case of non-linear models - is also raised, for it is also related somehow to the bandwidth of the wave field. A sea-state is actually made of a superimposition of various wave systems (as swells and wind-sea). It is then possible to refine even more its description, namely through the multi-system approach. The latter enables indeed to track in time and space the wave systems independently of each other. An algorithm for the automatic spatiotemporal analysis is proposed and illustrated to this end. Lastly, the influence of such description refinements (wave groupiness and multi-system approach) is observed at several locations (Portugal, North Sea, California) by considering various WEC models (linear axi-symmetric heaving devices, 3D SEAREV with non-linear hydrodynamics): in all cases, it is shown that both extended descriptions allow for a better characterisation of the mean WEC performance at both short and long term. Ces travaux portent sur la caractérisation spectrale des états de mer dans le cadre du développement de systèmes récupérateurs de l'énergie des vagues (SREVs). Jusqu'alors, cette caractérisation s'est limitée à des paramètres globaux basiques tels que la hauteur significative et la période moyenne des vagues. Les présents travaux soulignent notamment, à l'aide de modèles simplifiés, que cette description est insuffisante car un SREV montre encore une grande variabilité en termes de production moyenne, ces deux paramètres étant fixés par ailleurs. On s'intéresse donc à raffiner cette description en considérant des caractéristiques de vagues nouvelles, comme le groupement de vagues, lui-même lié à la largeur de bande de l'état de mer. Les approches classiques - spectrales - sur l'étude descriptive des groupes de vagues et la littérature permettent de dégager un certain nombre de paramètres spectraux. On observe ensuite la sensibilité de modèles de SREVs offshore simplifiés (modèles fréquentiels linéaires) à ces paramètres de largeur globale. La question de l'estimation déterministe de performance moyenne (dans le cas de convertisseurs non-linéaires) est également abordée, étant elle-même liée à cette caractéristique. Un état de mer étant constitué de plusieurs systèmes de vagues (houles, mer de vent), il est encore possible de raffiner sa description grâce à une approche multisystème. Celle-ci permet en outre de suivre ces systèmes indépendamment dans le temps et l'espace. Un algorithme d'analyse spatio-temporelle est proposé et illustré à cet égard. Enfin, l'influence de ces raffinements est observée en différents sites (Portugal, Mer du Nord, Californie) sur des cas de SREVs divers (systèmes axi-symétriques linéaires, SEAREV 3D d'hydrodynamique non-linéaire), et montre que dans tous les cas, le raffinement apporté permet de mieux caractériser la production à court et long terme de ces dispositifs.

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    Authors: Maestrini, S; Rochet, M; Legendre, L; Demers, S;

    Nutrient analyses were done in melted ice, frazil and sea water of the upper part of the water column, where as major constitutive elements of micro-algae were analysed. Measurements of nutrient uptake were carried out with frazil algae incubated at -1.5 degree C in a field incubator. Differential-enrichment bioassays and mean N/P ratio (5.9) demonstrated that actual growth of the cultures was nutrient-limited. Nitrogen governed the algal yield when illumination and grazing allowed the microalgae to grow. It concluded that the epontic dynamics is not only controlled from above, by the seasonal (climatic) changes in light intensity as generally assumed, but also from below, by the short-term (hydrodynamic) events of vertical mixing that replenish the ice-water interface in nutrients.

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    Authors: Koutou, Mahamoudou; Sangaré, Mamadou; Havard, Michel; Toillier, Aurélie; +3 Authors

    Income source and accompaniment needs of family farms in the cotton growing area of West Burkina Faso Description of the subject. In the western cotton growing area of Burkina Faso, the variability and the diversity of the income levels of family farms (EAFs) mean that these farms require suitable agronomic accompaniment. Objectives. This article gives an account of this diversity and the need for accompaniment according to the type of identified EAF.Method. The data were collected by the active method of participative research and by investigation of the practices of 120 farms in the commune of Koumbia. Principal component analyses with economic indicators of the EAF were applied, followed by an ascending hierarchical clustering. The structural characteristics, the agro-pastoral practices of production, and the perceptions of the EAF regarding the constraints and appropriateness of agricultural production were compared by variance analysis. Results. The results showed three types of farm: farms with a low-income culture (farmers with few animals), those with a high income culture (farmers with a large number of animals) and those deriving income from breeding (stockbreeders). Taking into account the structure of the farms, their production practices, their technico-economic performance and their appropriateness of agricultural production, the form of sustainable accompaniment needed may be identified in relation to several common or specific innovations of the identified farm types. Accompaniments in the case of farm stock management, and good practice in the management of soil fertility and of animals were common to the three types of EAF. The fodder culture, fattening on pasture and the method of dairy production were specific to the farmers with a large number of animals and to the stockbreeders. The improvement of the food value of culture residues and of some aspects of the maintenance of oxen were more appropriate to the farmers with few animals. Conclusions. In order to succeed, the process of accompaniment must take into account both these specificities and the concept of sustainability. Description du sujet. Dans la zone cotonnière ouest du Burkina Faso, la variabilité et la diversité des revenus des exploitations agricoles familiales (EAF) demandent pour ces dernières des accompagnements appropriés. Objectifs. Cet article rend compte de cette diversité et des besoins d’accompagnement selon les types d’EAF identifiés.Méthode. Les données ont été collectées par la méthode active de recherche participative et par enquête auprès d’un échantillon raisonné de 120 exploitations dans la commune de Koumbia. Une analyse en composante principale avec des indicateurs économiques de l’EAF suivie d’une classification ascendante hiérarchique ont été appliquées. Les caractéristiques structurelles, les pratiques de production agro-pastorales, les perceptions des chefs d’EAF sur les contraintes et opportunités de production agropastorale ont été comparées par une analyse de variance.Résultats. Les résultats montrent trois types d’exploitations : les exploitations à faible revenu culture (agriculteurs), à fort revenu culture (agro-éleveurs) et à revenu élevage (éleveurs). À la lumière de leurs structures, pratiques de production, performances technico-économiques et opportunités de production agricole, leur accompagnement peut porter sur plusieurs innovations durables communes ou spécifiques des types identifiés. Des accompagnements sur la gestion des ressources de l’exploitation, les bonnes pratiques de gestion de la fertilité des sols et des animaux sont communs aux trois types d’EAF. Les cultures fourragères, l’embouche et la production laitière sont spécifiques aux agro-éleveurs et éleveurs. L’amélioration de la valeur nutritive des résidus de cultures et l’entretien des bœufs de trait conviennent plus aux agriculteurs.Conclusions. Pour réussir, le processus d’accompagnement doit prendre en compte ces spécificités et la notion de durabilité.

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    Biotechnologie, Agronomie, Société et Environnement
    Article . 2016 . Peer-reviewed
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    Article . 2016
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      Biotechnologie, Agronomie, Société et Environnement
      Article . 2016 . Peer-reviewed
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  • Authors: Vichard, Loic;

    Ces modélisations, sont associées au projet AsDeCoEUR (Analyses De Composants Energétiques en Usage Réel). Le projet souhaite s’appuyer sur une démarche menée autour d’un travail de thèse visant à comprendre le comportement dynamique, d’étudier le vieillissement et d’estimer l’état de santé des composants énergétiques, batterie et pile à combustible en usage réel. Cette démarche est basée sur l’analyse des données enregistrées sur ces composants dans le projet Mobypost (flotte de 10 véhicules Hydrogènes). La mise en œuvre des compétences et des outils d’analyse numériques développés notamment pour les batteries par le laboratoire Ampère et l’IFSTTAR vise à comprendre et à reproduire les phénomènes de dégradation des composants étudiés. Ces modèles permettent de contribuer à l’étude du vieillissement et d’estimer l’état de santé en usage réel des batteries et des piles à combustible. These models are associated with the project AsDeCoEUR (Analyses De Composants Energétiques en Usage Réel). The project is based on a thesis work aiming at understanding the dynamic behavior, to study the aging and to estimate the health status of the battery and fuel cell energy components in real use. This approach is based on the analysis of data recorded on these components in the Mobypost project (fleet of 10 hydrogen vehicles). The implementation of skills and numerical analysis tools developed in particular for batteries by the Ampère laboratory and IFSTTAR aims to understand and reproduce the degradation phenomena of the components studied. These models contribute to the study of ageing and estimate the state of health of batteries and fuel cells in real use. AdditionnalInformation: Model produced within the framework of Loic Vichard's thesis in electrical engineering (Contribution to the study of the aging of battery and fuel cell components in real use), under the supervision of Daniel Hissel (PU at the FEMTO-ST laboratory), Alexandre Ravey (MCF at UTBM) and Pascal Venet (PU at the Claude Bernard Lyon 1 University). AdditionnalInformation: Modèle produit dans le cadre de la thèse en génie électrique de Loic Vichard (Contribution à l'étude du vieillissement des composantes batteries et pile à combustible en usage réel), sous la direction de Daniel Hissel (PU au laboratoire FEMTO-ST), Alexandre Ravey (MCF à l'UTBM) et Pascal Venet (PU à l'université Claude Bernard Lyon 1). Données computationnelles ou de simulation: - Langage de programmation : Langage Matlab et Python - Plate-forme/OS : Microsoft Windows, Mac OS, Linux,... - Version : MATLAB R2018a - Statut de développement : Actif Simulation or computational data: - Programming language: Matlab language et Python language - Platform/OS: Microsoft Windows, Mac OS, Linux,... - Version: MATLAB R2018a - Development status: Active Project: Thèse de doctorat en génie électrique - Loic Vichard, Contrat doctoral - Projet AsDeCoEUR (Region Bourgogne Franche-Comté) Label: PHE (Plateforme Hydrogène-Energie) UpdatePeriodicity: no update Audience: Research

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    Authors: Brunel, Thomas;

    Le recrutement - nombre de jeunes individus issus de la reproduction qui contribuent annuellement au renouvellement des stocks de poissons exploités - est un facteur clé de la dynamique de ces stocks. La forte variabilité du recrutement de la plupart des populations est liée en grande partie à l'influence des facteurs de l'environnement sur la survie lors des premiers stades de développement. La compréhension du déterminisme de cette variabilité est primordiale pour une gestion efficace des stocks. Cette thèse avait pour objectif de généraliser un certain nombre de points concernant l'influence de l'environnement sur le recrutement, par l'utilisation d'une approche macroécologique portant sur l'ensemble des populations exploitées de l'Atlantique Nord-Est. Le premier chapitre de la thèse répertorie les différentes sources de données de recrutement disponibles, évalue leur cohérence et leur fiabilité. Les estimations du recrutement issues des modèles d'analyse de cohortes, concernant plus de 60 populations de 18 espèces, sont finalement retenues pour l'analyse de la variabilité du recrutement. Le second chapitre présente les concepts et les méthodes d'analyse de la variabilité du recrutement. La synthèse des grandes hypothèses du déterminisme du recrutement permet de préciser les influences respectives des facteurs environnementaux et des autres facteurs sur la variabilité du recrutement. Les relations stock/recrutement sont ensuite présentées, en partant du cas concret des populations considérées dans cette étude. Le chapitre se termine par la présentation des différentes voies d'étude de la variabilité du recrutement et la justification du choix d'une approche macroécologique. Le troisième chapitre analyse les corrélations des variations du recrutement entre les populations et met en évidence des ensembles de populations dont les variations du recrutement ont été synchrones. Ces synchronismes représentent des communautés de réponse du recrutement des populations à l'influence des facteurs environnementaux et témoignent donc du contrôle environnemental des variations du recrutement. Le quatrième chapitre analyse les différences de variabilité du recrutement entre les populations. Il est notamment montré que les populations situées près des limites Nord et Sud des aires de répartition géographique des espèces présentent une variabilité du recrutement plus forte que celles situées au centre de ces aires. Cette relation est cohérente avec les théories de la macroécologie, selon lesquelles les populations en limite de distribution seraient affectées de façon plus marquée par les fluctuations des conditions environnementales qu'elles rencontrent. Le cinquième chapitre examine la correspondance entre les variations à long terme du recrutement et les changements récents du climat dans l'Atlantique Nord. La tendance dominante du recrutement de 40 populations durant les trente dernières années est fortement corrélée au signal dominant des variations de la température de surface du Nord-Est Atlantique, lui-même corrélé à la cinétique globale du réchauffement climatique. Ce chapitre suggère également que la réponse des populations à ce réchauffement pourrait dépendre de leur position dans les aires de répartition des espèces. Recruitment - the number of young fishes that integrate each year the exploitable stock¿ has a central importance for stock dynamics. The high temporal variability in recruitment is a result of the environmental influence on survival during early life stages. Understanding the determinism of the influence of environment on recruitment remains one of the most important question in fisheries ecology. The present work aimed at answering some general questions about the influence of environment on fish recruitment using a macroecological approach. The study focused on the exploited fish populations of the Northeastern Atlantic. The first chapter of the thesis makes a review of the different methods used to estimate recruitment, compares recruitment estimates from these different methods, and discusses their reliability. Recruitment estimates from cohort analysis models, concerning more than 60 populations of 18 species, are finally retained to study recruitment variability. The second chapter presents the main concepts and the different approaches to the study of recruitment variability. A review of the major hypotheses concerning recruitment determinism is first made in order to detail the respective influence of environmental factors and of other factors on recruitment variability. The link between stock size and recruitment is then presented, based on the analysis of the data concerning the populations considered in this work. The chapter ends with a presentation of the different approaches used to study recruitment variability, and the justification of the choice of a macroecological approach. The third chapter analyses correlations in recruitment variations among Northeast Atlantic fish stocks, and shows the existence of groups of populations with synchronous recruitment variations. Such synchronies in recruitment correspond to similar responses of recruitment to environmental factors and hence indicate an environmental control of recruitment variations. The fourth chapter analyses the differences in recruitment variability among populations. Recruitment variability for populations living near the borders of species distribution ranges is higher than for populations living at the centre of these ranges. According to macroecological theories this pattern of recruitment variability within species geographical ranges suggests that populations on the border of species range are more strongly influenced by environmental variability than populations at the centre of the range. The fifth chapter investigates coherence between long term trends in recruitment and the changes in the North Atlantic climate during the last three decades. The dominant trend of recruitment variations for 40 populations, extracted by a principal component analysis, is strongly negatively correlated with the dominant signal in sea surface temperature in the North-East Atlantic, which is highly correlated to the global increase in temperature related to global warming. The chapter also suggests that the impact of the global warming on fish recruitment may depend on population position in species range.

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    Authors: Boullet, Antoine;

    When it comes to sustainable development, n-butanol production by biological means represents an environmentally friendly alternative compared to petrochemical means. Some organisms are able to produce butanol naturally, such as the Clostridiae family, which are Gram-positive bacteria, growing in strict anaerobic conditions. They produce butanol in mixture with acetone and ethanol, which reduce the production yield of butanol and increase the purification costs. In 2015, the PEEP team of TBI built a genetically modified strain of E. coli, expressing the complete conversion pathway from pyruvate to butanol from C. acetobutylicum. This strain’s metabolism was built so that the growth and glucose consumption speeds are coupled with the butanol production speed. In strict anaerobic growth in batch culture, in a chemically defined medium based on glucose and supplemented with yeast extracts (YE) and nitrate (NO3-), this strain produces 3,3 g/L of n-butanol, with a yield of 0,23 g butanol/g glucose, among other minority coproducts (succinate, lactate, acetate, butyrate). These coproducts lower the butanol production yield, and remain unexpected, because the corresponding metabolic pathways were deleted. The goal of this PhD project consists in identifying the metabolic pathways involved in the coproducts production and improving the understanding of the strain, in order to optimize it, improve the butanol/glucose yield, and simplify the culture medium. Several aspects are considered: expressing ferredoxin NAD(P)+ oxidoreductases (FNOR) from organisms other than C. acetobutylicum, in order to combine the reoxidation of the reduced ferredoxin to the generation of ATP; ii) inactivating the metabolic pathways from E. coli which may be involved in the coproducts synthesis; iii) performing an evolutionary in-vivo adaptation of the strain, in order to improve its performances and increase its tolerance to butanol in a chemically defined medium supplemented with YE and NO3-, and then iv) performing an evolutionary adaptation of the Butanol strain to allow its growth in the chemically defined medium without any supplementation. These strategies lead to i) the construction of a new E. coli strain, with its anaerobic growth depending on the functionality of an FNOR: the FNOR from C. acetobutylicum, the Rnf complex from C. ljungdahlii and the FNOR from C. tepidum were assessed. This work lead to the selection of a mutant of the FNOR from C. tepidum, which has a ferredoxin NAD+ reductase activity 2,2 times higher than the native enzyme. This mutant FNOR was then expressed in the E. coli strain producing butanol, as a replacement of C. acetobutylicum’s. The characterization of its phenotype demonstrated its capacity to produce 3 g/L of butanol, with a yield of 0,26 g/g; ii) the deletion of the genes zwf and mdh in the Butanol strain also lead to an improvement of the production in butanol up to 6 g/L , and of the yield up to 0,33 g/g; iii) the in-vivo evolution of the Butanol strain, in continuous culture (chemostat) with regulated pH, in the medium supplemented with YE and Ni, lead to the selection of an evolved strain producing up to 11 g/L of butanol, and a yield of 0,34 g/g. The complete sequencing of the genomes of the clones isolated from this population lead to the identification of mutations in two genes, yqhC and lptG, potentially involved in the strain’s performances; iv) the in-vivo adaptation of the Butanol strain in the medium without any supplementation lead to the selection of a evolved clones producing 2,5 g/L of butanol, and a yield of 0,28 g/g in batch culture. To our knowledge, the highest production of butanol observed coupled with anaerobic growth in E. coli does not exceed 0,6g/L, in a chemically defined medium base on glucose without any supplementation.; Dans le cadre du développement durable, la production de n-butanol par voie biologique est une alternative écologique par rapport à sa synthèse par voie pétrochimique. Il existe des microorganismes naturellement capables de produire du butanol, tels que les Clostridiae, bactéries Gram positif, ayant une croissance strictement anaérobie. Ils produisent du butanol au travers d’une fermentation produisant un mélange d’acétone, de butanol et d’éthanol. La production de butanol en mélange avec d’autres molécules diminue le rendement de production en butanol et augmente le coût de purification. En 2015, l’équipe PEEP de TBI a construit une souche d’E. coli génétiquement modifiée exprimant la voie complète de conversion du pyruvate en n-butanol de C. acetobutylicum. Le métabolisme de cette souche a été conçu pour que les vitesses de croissance et de consommation du glucose soient couplées à la vitesse de production du butanol. En croissance en anaérobiose stricte en culture discontinue dans un milieu chimiquement défini à base de glucose supplémenté en extrait de levure (YE) et nitrate (NO3-), cette souche produit 3,3 g/L de butanol à un rendement de 0,23 g butanol /g glucose, en présence de coproduits minoritaires (succinate, lactate, acétate, butyrate). Ces coproduits diminuent le rendement de production en butanol et restent inattendus car les voies métaboliques correspondantes ont été supprimées. L’objectif de ce projet de thèse est d’identifier les voies métaboliques impliquées dans la production de ces coproduits et d’améliorer la compréhension du métabolisme de cette souche, afin de l’optimiser, d’augmenter le rendement butanol/glucose, et de simplifier le milieu de culture. Plusieurs pistes ont été envisagées : i) Expression de ferrédoxines NAD(P)+ oxydoréductases (FNOR) issues d’organismes autres que C. acetobutylicum dans l’objectif de coupler l’étape de réoxydation de la ferrédoxine réduite à la production d’ATP ; ii) Inactivation des voies métaboliques d’E. coli susceptibles d’être impliquées dans la synthèse des coproduits ; iii) Evolution adaptative in vivo de la souche, afin d’améliorer ses performances et accroitre sa tolérance au butanol dans le milieu supplémenté avec YE et NO3-, puis iv) Evolution adaptative in vivo de la souche dans le milieu sans supplémentation. Ces stratégies ont conduit à i) La construction d’une nouvelle souche d’E. coli dont la croissance anaérobie dépend de la fonctionnalité d’une FNOR : la FNOR de C. acetobutylicum, le complexe Rnf de C. ljungdahlii et la FNOR de C. tepidum ont été évaluées. Cette approche a conduit à la sélection d’un mutant de la FNOR de C. tepidum, ayant une activité ferrédoxine NAD+ réductase 2,2 fois supérieure à celle de l’enzyme native. Cette FNOR mutante a ensuite été exprimée dans la souche d’E. coli Butanol en remplacement de la FNOR de C. acetobutylicum. La caractérisation de son phénotype a montré sa capacité à produire 3 g/L de butanol à un rendement de 0,26 g/g ; ii) La délétion des gènes zwf et mdh dans la souche Butanol a permis d’augmenter le titre en butanol produit à 6 g/L et le rendement à 0,33 g/g ; iii) L’évolution adaptative in-vivo de la souche d’E. coli Butanol en culture continue, dans le milieu supplémenté avec YE et Ni, a conduit à la sélection d’une population évoluée produisant 11 g/L de butanol à un rendement de 0,34 g/g. Le séquençage complet des génomes de clones isolés à partir de cette population a conduit à l’identification de mutations dans deux gènes, yqhC et lptG, potentiellement impliquées dans les performances de la souche ; iv) L’évolution adaptative in-vivo de la souche Butanol dans le milieu sans supplémentation a conduit à la sélection de clones évolués produisant 2,5 g/L de butanol à un rendement de 0,28 g/g en culture discontinue. A notre connaissance, la production maximale de butanol reportée, couplée à la croissance anaérobie chez E. coli, ne dépasse pas 0,6 g/L, en milieu chimiquement défini à base uniquement de glucose.

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