• Energy Research

Microalgae-based treatment can be applied to the bioremediation of agro-industrial wastewater, aiming at a circular economy approach. The present work compared the technical-environmental feasibility of operating a bubble column photobioreactor (PBR) and a high rate pond (HRP) for microalgae biomass production and wastewater treatment of a meat processing facility. The comparison was made regarding biomass productivity, phytoplankton composition, treatment efficiency, life cycle assessment, and energy balance. The daily yields of total biomass and the maximum specific growth rates were 483.33 mg L-1 d-1 and 0.23 d-1 for PBR and 95.00 mg L-1·d-1 and 0.193 d-1 for HRP, respectively, with a predominance of the species Scenedesmus acutus. The treatment efficiency of COD (~50%) and phosphorus (100%) were similar in the two reactors. However, the PBR showed greater assimilation of ammoniacal nitrogen (100% removal) due to the higher microalgal biomass productivity. Environmental impacts were assessed through the ReCiPe methodology for midpoint and endpoint levels. Results revealed that CO2 supply was the most impactful process for both systems (>60%), but HRP reached lower environmental burdens (-105.90 mPt) than PBR (60.74 mPt). Energy balance through the Net Energy Ratio also resulted in the HPR advantage over the PBR (NER = 14.23 and 1.09, respectively). Still, both reactors present advantages when applied to different valorization routes. At the same time, both present room for improvement in the light of bioeconomy and biorefineries, aiming at sustainable wastewater treatment plants.

Microalgae are a potential feedstock for several bioproducts, mainly from its primary and secondary metabolites. Lipids can be converted in high-value polyunsaturated fatty acids (PUFA) such as omega-3, carbohydrates are potential biohydrogen (bioH2) sources, proteins can be converted into biopolymers (such as bioplastics) and pigments can achieve high concentrations of valuable carotenoids. This work comprehends the current practices for the production of such products from microalgae biomass, with insights on technical performance, environmental and economical sustainability. For each bioproduct, discussion includes insights on bioprocesses, productivity, commercialization, environmental impacts and major challenges. Opportunities for future research, such as wastewater cultivation, arise as environmentally attractive alternatives for sustainable production with high potential for resource recovery and valorization. Still, microalgae biotechnology stands out as an attractive topic for it research and market potential.

Microalgae biomass has attracted attention as a feedstock to produce biofuels, biofertilizers, and pigments. However, the high production cost associated with cultivation and separation stages is a challenge for the microalgae biotechnology application on a large scale. A promising approach to overcome the technical-economic limitations of microalgae production is using wastewater as a nutrient and water source for cultivation. This strategy reduces cultivation costs and contributes to valorizing sanitation resources. Therefore, this article presents a comprehensive literature review on the status of microalgae biomass cultivation in wastewater, focusing on production strategies and the accumulation of valuable compounds such as lipids, carbohydrates, proteins, fatty acids, and pigments. This review also covers emerging techniques for harvesting microalgae biomass cultivated in wastewater, discussing the advantages and limitations of the process, as well as pointing out the main research opportunities. The novelty of the study lies in providing a detailed analysis of state-of-the-art and potential advances in the cultivation and harvesting of microalgae, with a special focus on the use of wastewater and implementing innovative strategies to enhance productivity and the accumulation of compounds. In this context, the work aims to guide future research concerning emerging technologies in the field, emphasizing the importance of innovative approaches in cultivating and harvesting microalgae for advancing knowledge and practical applications in this area.

Municipal wastewater (MW) and industrial wastewater from juice processing (IWJ) were blended in different proportions to assess the effect of the carbon/nitrogen (C/N) ratio on pollutant removal, microalgal biomass (MB) cultivation, and the accumulation of carotenoids and biocompounds. MB development was not observed in treatments with higher C/N ratios (>30.67). The wastewater mixture favored the removal of dissolved organic carbon (75.61 and 81.90%) and soluble chemical oxygen demand (66.78-88.85%), compared to the treatment composed exclusively of MW (T7). Treatments T3 and T6 (C/N ratio equal to 30.67 and 7.52, respectively) showed higher Chlorophyll-a concentrations, 1.47 and 1.54 times higher than T7 (C/N ratio 1.75). It was also observed that the C/N ratio of 30.67 favored the accumulation of carbohydrates and lipids (30.07% and 26.39%, respectively), while the C/N ratio of 7.52 improved protein accumulation (33.00%). The fatty acids C16:0, C18:1, C18:2, and C18:3 had the highest concentrations. Additionally, increasing the C/N ratio can be an efficient strategy to improve the production of fatty acids for biofuels, mainly due to the increased concentration of shorter-chain fatty acids (C16:0). These findings suggest that blending wastewater not only enhances treatment performance but also increases the accumulation of valuable carbohydrates and lipids in MB, and optimizes fatty acid production for biofuel applications. This research represents significant progress towards feasibility of using MB produced from wastewater.

Microalgae biomass is a versatile feedstock with a variable composition that can be submitted to several conversion routes. Considering the increasing energy demand and the context of third-generation biofuels, algae can fulfill the increasing global demand for energy with the additional benefit of environmental impact mitigation. While biodiesel and biogas are widely consolidated and reviewed, emerging algal-based biofuels such as biohydrogen, biokerosene, and biomethane are cutting-edge technologies in earlier stages of development. In this context, the present study covers their theoretical and practical conversion technologies, environmental hotspots, and cost-effectiveness. Scaling-up considerations are also addressed, mainly through Life Cycle Assessment results and interpretation. Discussions on the current literature for each biofuel directs researchers towards challenges such as optimized pretreatment methods for biohydrogen and optimized catalyst for biokerosene, besides encouraging pilot and industrial scale studies for all biofuels. While presenting studies for larger scales, biomethane still needs continuous operation results to consolidate the technology further. Additionally, environmental improvements on all three routes are discussed in light of life-cycle models, highlighting the ample research opportunities on wastewater-grown microalgae biomass.