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description Publicationkeyboard_double_arrow_right Article , Journal 2017 BelgiumPublisher:Oxford University Press (OUP) Funded by:EC | P-MEDICINEEC| P-MEDICINEWannes Voorend; Wout Boerjan; Dharshana Padmakshan; Dharshana Padmakshan; Andreas Pallidis; José Nicomedes Junior; José Nicomedes Junior; Geert Goeminne; Hoon Kim; Hoon Kim; Lívia Vargas; Jan Van Doorsselaere; Fernando Campos de Assis Fonseca; Marina de Lyra Soriano Saleme; John Ralph; John Ralph; Ruben Vanholme; Rebecca Van Acker; Igor Cesarino; Igor Cesarino;Caffeoyl shikimate esterase (CSE) was recently shown to play an essential role in lignin biosynthesis in Arabidopsis (Arabidopsis thaliana) and later in Medicago truncatula However, the general function of this enzyme was recently questioned by the apparent lack of CSE activity in lignifying tissues of different plant species. Here, we show that down-regulation of CSE in hybrid poplar (Populus tremula × Populus alba) resulted in up to 25% reduced lignin deposition, increased levels of p-hydroxyphenyl units in the lignin polymer, and a relatively higher cellulose content. The transgenic trees were morphologically indistinguishable from the wild type. Ultra-high-performance liquid chromatography-mass spectrometry-based phenolic profiling revealed a reduced abundance of several oligolignols containing guaiacyl and syringyl units and their corresponding hydroxycinnamaldehyde units, in agreement with the reduced flux toward coniferyl and sinapyl alcohol. These trees accumulated the CSE substrate caffeoyl shikimate along with other compounds belonging to the metabolic classes of benzenoids and hydroxycinnamates. Furthermore, the reduced lignin amount combined with the relative increase in cellulose content in the CSE down-regulated lines resulted in up to 62% more glucose released per plant upon limited saccharification when no pretreatment was applied and by up to 86% and 91% when acid and alkaline pretreatments were used. Our results show that CSE is not only important for the lignification process in poplar but is also a promising target for the development of improved lignocellulosic biomass crops for sugar platform biorefineries.
PLANT PHYSIOLOGY arrow_drop_down Ghent University Academic BibliographyArticle . 2017Data sources: Ghent University Academic Bibliographyadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess Routesbronze 96 citations 96 popularity Top 1% influence Top 10% impulse Top 1% Powered by BIP!
more_vert PLANT PHYSIOLOGY arrow_drop_down Ghent University Academic BibliographyArticle . 2017Data sources: Ghent University Academic Bibliographyadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1104/pp.17.00920&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article 2023 United StatesPublisher:Wiley Taichi E. Takasuka; Hoon Kim; Kai Deng; Christopher M. Bianchetti; Kaho Yamashita; Emily T. Beebe; Lai F. Bergeman; Kirk A. Vander Meulen; Samuel Deutsch; John Ralph; Paul D. Adams; Trent R. Northen; Brian G. Fox;pmid: 37402642
AbstractKelp is an abundant, farmable biomass‐containing laminarin and alginate as major polysaccharides, providing an excellent model substrate to study their deconstruction by simple enzyme mixtures. Our previous study showed strong reactivity of the glycoside hydrolase family 55 during hydrolysis of purified laminarin, raising the question of its reactivity with intact kelp. In this study, we determined that a combination of a single glycoside hydrolase family 55 β‐1,3‐exoglucanase with a broad‐specificity alginate lyase from the polysaccharide lyase family 18 gives efficient hydrolysis of untreated kelp to a mixture of simple sugars, that is, glucose, gentiobiose, mannitol‐end glucose, and mannuronic and guluronic acids and their soluble oligomers. Quantitative assignments from nanostructure initiator mass spectrometry (NIMS) and 2D HSQC NMR spectroscopy and analysis of the reaction time‐course are provided. The data suggest that binary combinations of enzymes targeted to the unique polysaccharide composition of marine biomass are sufficient to deconstruct kelp into soluble sugars for microbial fermentation.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2023License: CC BY NC NDFull-Text: https://escholarship.org/uc/item/8421v8cqData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2023Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess RoutesGreen hybrid 4 citations 4 popularity Top 10% influence Average impulse Average Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2023License: CC BY NC NDFull-Text: https://escholarship.org/uc/item/8421v8cqData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2023Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1002/cbic.202300357&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type , Journal 2015 BelgiumPublisher:Springer Science and Business Media LLC Funded by:UKRI | RootDetect: Remote Detect..., NSF | Purchase of an Electrospr...UKRI| RootDetect: Remote Detection and Precision Management of Root Health ,NSF| Purchase of an Electrospray Ionization Mass SpectrometerFachuang Lu; Steven D. Karlen; Matt Regner; Hoon Kim; Sally A. Ralph; Run Cang Sun; Ken-ichi Kuroda; Mary Ann Augustin; Raymond Mawson; Henry Sabarez; Tanoj K. Singh; Gerardo Jimenez-Monteon; Sarani Zakaria; Stefan J. Hill; Philip J. Harris; Wout Boerjan; Curtis G. Wilkerson; Shawn D. Mansfield; John Ralph;handle: 1854/LU-8637689
La production industrielle d'huile de palme génère simultanément une quantité substantielle de fibres de grappe de fruits vides (EFB) qui pourraient être utilisées comme matière première dans une bioraffinerie à base de lignocellulose. Les sous-produits de la lignine générés par ce processus peuvent offrir des possibilités d'isolation des produits à valeur ajoutée, tels que le p-hydroxybenzoate (pBz), pour aider à compenser les coûts d'exploitation. L'analyse de la lignine EFB par spectroscopie de résonance magnétique nucléaire (RMN) a clairement révélé la présence d'acétate lié et de pBz, la saponification révélant que 1,1 % en poids de l'EFB était du pBz ; avec une teneur en lignine de 22,7 %, 4,8 % de la lignine est du pBz qui peut être obtenu comme composant pur pour être utilisé comme charge chimique. L'analyse de la lignine EFB par RMN et dérivatisation suivie d'un clivage réducteur (DFRC) a montré que le pBz acylate sélectivement le groupe γ-hydroxyle des unités S. Cette sélectivité suggère que le pBz, de manière analogue à l'acétate dans le kénaf, le p-coumarate dans les graminées et le ferrate dans un peuplier transgénique augmenté d'une féruloyl-CoA monolignol transférase (FMT), est incorporé dans la chaîne de lignine en croissance via son conjugué monolignol γ-p-hydroxybenzoylé. L'implication de tels conjugués dans la lignification des palmiers est prouvée par l'observation de nouvelles unités couplées au β-β non résinol p-hydroxybenzoylé dans les lignines. Ensemble, les données impliquent l'existence de p-hydroxybenzoyl-CoA :monolignol transférases qui sont impliquées dans la lignification chez les différents saules (Salix spp.), peupliers et peupliers faux-trembles (Populus spp., famille des Salicacées) et palmiers (famille des Arecacées) qui ont des lignines p-hydroxybenzoylées. Même sans augmenter les niveaux par la sélection ou le génie génétique, les « déchets » actuels de l'EFB d'huile de palme devraient être en mesure de générer un flux important d'acide p-hydroxybenzoïque qui offre des opportunités pour le développement de produits à valeur ajoutée dérivés de l'industrie du palmier à huile. La producción industrial de aceite de palma genera simultáneamente una cantidad sustancial de fibras de racimo de frutas vacías (EFB) que podrían utilizarse como materia prima en una biorrefinería a base de lignocelulosa. Los subproductos de lignina generados por este proceso pueden ofrecer oportunidades para el aislamiento de productos de valor agregado, como el p-hidroxibenzoato (pBz), para ayudar a compensar los costos operativos. El análisis de la lignina EFB por espectroscopía de resonancia magnética nuclear (RMN) reveló claramente la presencia de acetato unido y pBz, y la saponificación reveló que el 1,1% en peso del EFB era pBz; con un contenido de lignina del 22,7 %, el 4,8 % de la lignina es pBz que se puede obtener como un componente puro para su uso como materia prima química. El análisis de la lignina EFB por RMN y la derivatización seguida de escisión reductora (DFRC) mostró que pBz acila selectivamente el grupo γ-hidroxilo de las unidades S. Esta selectividad sugiere que pBz, análogamente con acetato en kenaf, p-cumarato en gramíneas y ferular en un álamo transgénico aumentado con una feruloil-CoA monolignol transferasa (FMT), se incorpora a la cadena de lignina en crecimiento a través de su conjugado de monolignol γ-p-hidroxibenzoilado. La participación de dichos conjugados en la lignificación de la palma se demuestra al observar nuevas unidades no acopladas a β-β-resinol p-hidroxibenzoiladas en las ligninas. Juntos, los datos implican la existencia de p-hidroxibenzoil-CoA:monolignol transferasas que participan en la lignificación en los diversos sauces (Salix spp.), álamos y álamo temblón (Populus spp., familia Salicaceae) y palmeras (familia Arecaceae) que tienen ligninas p-hidroxibenzoiladas. Incluso sin mejorar los niveles mediante mejoramiento o ingeniería genética, los 'desechos' actuales de aceite de palma EFB deberían ser capaces de generar una corriente considerable de ácido p-hidroxibenzoico que ofrezca oportunidades para el desarrollo de productos de valor agregado derivados de la industria de la palma aceitera. The industrial production of palm oil concurrently generates a substantial amount of empty fruit bunch (EFB) fibers that could be used as a feedstock in a lignocellulose-based biorefinery. Lignin byproducts generated by this process may offer opportunities for the isolation of value-added products, such as p-hydroxybenzoate (pBz), to help offset operating costs. Analysis of the EFB lignin by nuclear magnetic resonance (NMR) spectroscopy clearly revealed the presence of bound acetate and pBz, with saponification revealing that 1.1 wt% of the EFB was pBz; with a lignin content of 22.7 %, 4.8 % of the lignin is pBz that can be obtained as a pure component for use as a chemical feedstock. Analysis of EFB lignin by NMR and derivatization followed by reductive cleavage (DFRC) showed that pBz selectively acylates the γ-hydroxyl group of S units. This selectivity suggests that pBz, analogously with acetate in kenaf, p-coumarate in grasses, and ferulate in a transgenic poplar augmented with a feruloyl-CoA monolignol transferase (FMT), is incorporated into the growing lignin chain via its γ-p-hydroxybenzoylated monolignol conjugate. Involvement of such conjugates in palm lignification is proven by the observation of novel p-hydroxybenzoylated non-resinol β–β-coupled units in the lignins. Together, the data implicate the existence of p-hydroxybenzoyl-CoA:monolignol transferases that are involved in lignification in the various willows (Salix spp.), poplars and aspen (Populus spp., family Salicaceae), and palms (family Arecaceae) that have p-hydroxybenzoylated lignins. Even without enhancing the levels by breeding or genetic engineering, current palm oil EFB 'wastes' should be able to generate a sizeable stream of p-hydroxybenzoic acid that offers opportunities for the development of value-added products derived from the oil palm industry. ينتج الإنتاج الصناعي لزيت النخيل في الوقت نفسه كمية كبيرة من ألياف مجموعة الفاكهة الفارغة (EFB) التي يمكن استخدامها كمادة وسيطة في مصفاة حيوية تعتمد على اللجينوسليلوز. قد توفر المنتجات الثانوية لليجنين الناتجة عن هذه العملية فرصًا لعزل المنتجات ذات القيمة المضافة، مثل p - hydroxybenzoate (pBz)، للمساعدة في تعويض تكاليف التشغيل. كشف تحليل اللجنين EFB بواسطة مطياف الرنين المغناطيسي النووي (NMR) بوضوح عن وجود أسيتات مرتبطة و pBz، مع كشف التصبن أن 1.1 ٪ بالوزن من EFB كان pBz ؛ مع محتوى اللجنين بنسبة 22.7 ٪، 4.8 ٪ من اللجنين عبارة عن pBz يمكن الحصول عليه كمكون نقي للاستخدام كمادة وسيطة كيميائية. أظهر تحليل اللجنين EFB بواسطة NMR والاشتقاق متبوعًا بالانقسام الاختزالي (DFRC) أن pBz يعمل بشكل انتقائي على أسيتيل مجموعة γ - hydroxyl من وحدات S. تشير هذه الانتقائية إلى أن pBz، بالتناظر مع الأسيتات في الكناف، و p - comarate في الأعشاب، و ferulate في حور معدّل وراثيًا معززًا بـ feruloyl - CoA monolignol transferase (FMT)، يتم دمجه في سلسلة اللجنين المتنامية عبر γ - p - hydroxybenzoylated monolignol conjugate. ثبت تورط مثل هذه المترافقات في ترصيع النخيل من خلال ملاحظة وحدات p - hydroxybenzoylated غير الراتينول β - β المقترنة في اللجنين. معا، تشير البيانات إلى وجود p - hydroxybenzoyl - CoA: monolignol transferases التي تشارك في تبييض في الصفصاف المختلفة (Salix spp.)، الحور والحور (Populus spp.، عائلة Salicaceae)، والنخيل (عائلة Arecaceae) التي تحتوي على p - hydroxybenzoylated lignins. حتى من دون تعزيز المستويات عن طريق التكاثر أو الهندسة الوراثية، يجب أن تكون "نفايات" زيت النخيل الحالية قادرة على توليد تيار كبير من حمض p - hydroxybenzoic الذي يوفر فرصًا لتطوير منتجات ذات قيمة مضافة مشتقة من صناعة نخيل الزيت.
BioEnergy Research arrow_drop_down Ghent University Academic BibliographyArticle . 2015Data sources: Ghent University Academic Bibliographyadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess Routeshybrid 110 citations 110 popularity Top 1% influence Top 10% impulse Top 1% Powered by BIP!
more_vert BioEnergy Research arrow_drop_down Ghent University Academic BibliographyArticle . 2015Data sources: Ghent University Academic Bibliographyadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-015-9583-4&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2015Publisher:Springer Science and Business Media LLC Funded by:NSF | Fundamental Understanding..., UKRI | RootDetect: Remote Detect...NSF| Fundamental Understanding of HDA Process: One-Step Conversion of Lignocellulosic Biomass to Furan-Based Precursors for Drop-in Liquid Fuel ,UKRI| RootDetect: Remote Detection and Precision Management of Root HealthTae Hyun Kim; Chang Geun Yoo; Xuejun Pan; Hoon Kim; Ali Azarpira; Kyeong Keun Oh; John Ralph; John Ralph; Fachuang Lu; Jun Seok Kim;AbstractPhysicochemical characteristics of corn stover pretreated by soaking in aqueous ammonia (SAA) and low-moisture anhydrous ammonia (LMAA) were compared and investigated. The glucan digestibility of the treated biomass reached 90 % (SAA) and 84 % (LMAA). The LMAA pretreatment enhanced the digestibility by cleaving cross-linkages between cell wall components, whereas the SAA pretreatment additionally improved the digestibility by efficiently removing a major portion of the lignin under mild reaction conditions without significant loss of carbohydrates. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), and gel permeation chromatography (GPC) revealed the structural and chemical transformations of lignin during the pretreatments. Both pretreatments effectively cleaved ferulate cell wall cross-linking that is associated with the recalcitrance of grass lignocellulosics toward enzymatic saccharification. Extracted lignin from SAA pretreatment was extensively depolymerized but retained “native” character, as evidenced by the retention of β-ether linkages.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-015-9662-6&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routeshybrid 51 citations 51 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-015-9662-6&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2008Publisher:Springer Science and Business Media LLC Funded by:NSF | Development of 750 MHz Sp..., NSF | National Biological NMR F..., NIH | 900 MHz NMR Spectrometer ... +4 projectsNSF| Development of 750 MHz Spectroscopy at the National Magnetic Resonance Facility at Madison. ,NSF| National Biological NMR Facility at Madison ,NIH| 900 MHz NMR Spectrometer Biomolecular Investigations ,NIH| CONSOLE UPGRADES FOR NMR FACILITY ,NIH| WIDE-BORE 9.4 TESLA NMR SPECTROMETER FOR UW-MADISON ,NIH| MAINTENANCE OF NMRFAM SPECTROMETERS (MAGNET TESTING) ,UKRI| RootDetect: Remote Detection and Precision Management of Root HealthTakuya Akiyama; Hoon Kim; Hoon Kim; John Ralph; John Ralph;Although finely divided ball-milled whole cell walls do not completely dissolve in dimethylsulfoxide (DMSO), they readily swell producing a gel. Solution-state two-dimensional (2D) nuclear magnetic resonance (NMR) of this gel, produced directly in the NMR tube, provides an interpretable structural fingerprint of the polysaccharide and lignin components of the wall without actual solubilization, and without structural modification beyond that inflicted by the ball milling and ultrasonication steps. Since the cellulose is highly crystalline and difficult to swell, the component may be under-represented in the spectra. The method however provides a more rapid method for comparative structural evaluation of plant cell walls than is currently available. With the new potential for chemometric analysis using the 2D NMR fingerprint, this method may find application as a secondary screen for selecting biomass lines and for optimizing biomass processing and conversion efficiencies.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-008-9004-z&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu268 citations 268 popularity Top 1% influence Top 1% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-008-9004-z&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2017 Belgium, FrancePublisher:Oxford University Press (OUP) Funded by:EC | MULTIBIOPRO, EC | ENERGYPOPLAREC| MULTIBIOPRO ,EC| ENERGYPOPLARCatherine Lapierre; Françoise Laurans; Wout Boerjan; Geert Goeminne; Frédéric Legée; Annabelle Déjardin; Gilles Pilate; Lennart Hoengenaert; Cliff E. Foster; Sandrien Desmet; Hoon Kim; Hoon Kim; Ruben Vanholme; Kris Morreel; Rebecca Van Acker; Nicholas Santoro; John Ralph;In the search for renewable energy sources, genetic engineering is a promising strategy to improve plant cell wall composition for biofuel and bioproducts generation. Lignin is a major factor determining saccharification efficiency and, therefore, is a prime target to engineer. Here, lignin content and composition were modified in poplar (Populus tremula × Populus alba) by specifically down-regulating CINNAMYL ALCOHOL DEHYDROGENASE1 (CAD1) by a hairpin-RNA-mediated silencing approach, which resulted in only 5% residual CAD1 transcript abundance. These transgenic lines showed no biomass penalty despite a 10% reduction in Klason lignin content and severe shifts in lignin composition. Nuclear magnetic resonance spectroscopy and thioacidolysis revealed a strong increase (up to 20-fold) in sinapaldehyde incorporation into lignin, whereas coniferaldehyde was not increased markedly. Accordingly, ultra-high-performance liquid chromatography-mass spectrometry-based phenolic profiling revealed a more than 24,000-fold accumulation of a newly identified compound made from 8-8 coupling of two sinapaldehyde radicals. However, no additional cinnamaldehyde coupling products could be detected in the CAD1-deficient poplars. Instead, the transgenic lines accumulated a range of hydroxycinnamate-derived metabolites, of which the most prominent accumulation (over 8,500-fold) was observed for a compound that was identified by purification and nuclear magnetic resonance as syringyl lactic acid hexoside. Our data suggest that, upon down-regulation of CAD1, coniferaldehyde is converted into ferulic acid and derivatives, whereas sinapaldehyde is either oxidatively coupled into S'(8-8)S' and lignin or converted to sinapic acid and derivatives. The most prominent sink of the increased flux to hydroxycinnamates is syringyl lactic acid hexoside. Furthermore, low-extent saccharification assays, under different pretreatment conditions, showed strongly increased glucose (up to +81%) and xylose (up to +153%) release, suggesting that down-regulating CAD1 is a promising strategy for improving lignocellulosic biomass for the sugar platform industry.
PLANT PHYSIOLOGY arrow_drop_down Ghent University Academic BibliographyArticle . 2017Data sources: Ghent University Academic BibliographyInstitut National de la Recherche Agronomique: ProdINRAArticle . 2017Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1104/pp.17.00834&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesbronze 102 citations 102 popularity Top 1% influence Top 10% impulse Top 1% Powered by BIP!
more_vert PLANT PHYSIOLOGY arrow_drop_down Ghent University Academic BibliographyArticle . 2017Data sources: Ghent University Academic BibliographyInstitut National de la Recherche Agronomique: ProdINRAArticle . 2017Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article 2023Publisher:Royal Society of Chemistry (RSC) Feng Cheng; Sarah Liu; Steven D. Karlen; Hoon Kim; Fachuang Lu; John Ralph; Leida M. Vázquez Ramos; George W. Huber; James A. Dumesic;doi: 10.1039/d2gc03446h
In this paper, we describe an approach for producing both high quality and high quantity of lignin by studying structural change of lignin during treatment of poplar wood in γ-valerolactone (GVL) for a range of temperatures (80–120 °C) and reaction time at temperature (1–24 h).
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For further information contact us at helpdesk@openaire.eu29 citations 29 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
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For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type 2024Publisher:Springer Science and Business Media LLC Funded by:UKRI | RootDetect: Remote Detect...UKRI| RootDetect: Remote Detection and Precision Management of Root HealthFaride Unda; Lisanne de Vries; Steven D. Karlen; Jordan Rainbow; Chengcheng Zhang; Laura E. Bartley; Hoon Kim; John Ralph; Shawn D. Mansfield;Abstract Background The phenolic polymer lignin is one of the primary chemical constituents of the plant secondary cell wall. Due to the inherent plasticity of lignin biosynthesis, several phenolic monomers have been shown to be incorporated into the polymer, as long as the monomer can undergo radicalization so it can participate in coupling reactions. In this study, we significantly enhance the level of incorporation of monolignol ferulate conjugates into the lignin polymer to improve the digestibility of lignocellulosic biomass. Results Overexpression of a rice Feruloyl-CoA Monolignol Transferase (FMT), OsFMT1, in hybrid poplar (Populus alba x grandidentata) produced transgenic trees clearly displaying increased cell wall-bound ester-linked ferulate, p-hydroxybenzoate, and p-coumarate, all of which are in the lignin cell wall fraction, as shown by NMR and DFRC. We also demonstrate the use of a novel UV–Vis spectroscopic technique to rapidly screen plants for the presence of both ferulate and p-hydroxybenzoate esters. Lastly we show, via saccharification assays, that the OsFMT1 transgenic p oplars have significantly improved processing efficiency compared to wild-type and Angelica sinensis-FMT-expressing poplars. Conclusions The findings demonstrate that OsFMT1 has a broad substrate specificity and a higher catalytic efficiency compared to the previously published FMT from Angelica sinensis (AsFMT). Importantly, enhanced wood processability makes OsFMT1 a promising gene to optimize the composition of lignocellulosic biomass.
Biotechnology for Bi... arrow_drop_down Biotechnology for Biofuels and BioproductsArticle . 2024 . Peer-reviewedLicense: CC BYData sources: Crossrefhttps://doi.org/10.21203/rs.3....Article . 2024 . Peer-reviewedLicense: CC BYData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 1 citations 1 popularity Average influence Average impulse Average Powered by BIP!
more_vert Biotechnology for Bi... arrow_drop_down Biotechnology for Biofuels and BioproductsArticle . 2024 . Peer-reviewedLicense: CC BYData sources: Crossrefhttps://doi.org/10.21203/rs.3....Article . 2024 . Peer-reviewedLicense: CC BYData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type , Journal 2016Publisher:Springer Science and Business Media LLC Hoon Kim; Xuebin Zhang; Kewei Zhang; Yuanheng Cai; John Ralph; Chang-Jun Liu; Lisa M. Miller; Huijun Yang; Huan Feng; Huan Feng; Guichuan Hou;AbstractProducing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in the plant cell wall. Manipulating the monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs plant growth and development. Here, we show that expressing an engineered 4-O-methyltransferase that chemically modifies the phenolic moiety of lignin monomeric precursors, thus preventing their incorporation into the lignin polymer, substantially alters hybrid aspens’ lignin content and structure. Woody biomass derived from the transgenic aspens shows a 62% increase in the release of simple sugars and up to a 49% increase in the yield of ethanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation. Moreover, the cell wall structural changes do not affect growth and biomass production of the trees. Our study provides a useful strategy for tailoring woody biomass for bio-based applications.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 64 citations 64 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
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For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2012 Australia, DenmarkPublisher:Springer Science and Business Media LLC Kolby Hirth; Hoon Kim; John Ralph; Claus Felby; Prasad Kaparaju; Prasad Kaparaju; Daniel J. Yelle; Christopher G. Hunt;handle: 10072/67631
Solution-state two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy of plant cell walls is a powerful tool for characterizing changes in cell wall chemistry during the hydrothermal pretreatment process of wheat straw for second-generation bioethanol production. One-bond 13C–1H NMR correlation spectroscopy, via an heteronuclear single quantum coherence experiment, revealed substantial lignin β-aryl ether cleavage, deacetylation via cleavage of the natural acetates at the 2-O- and 3-O-positions of xylan, and uronic acid depletion via cleavage of the (1 → 2)-linked 4-O-methyl-α-d-glucuronic acid of xylan. In the polysaccharide anomeric region, decreases in the minor β-d-mannopyranosyl, and α-l-arabinofuranosyl units were observed in the NMR spectra from hydrothermally pretreated wheat straw. The aromatic region indicated only minor changes to the aromatic structures during the process (e.g., further deacylation revealed by the depletion in ferulate and p-coumarate structures). Supplementary chemical analyses showed that the hydrothermal pretreatment increased the cellulose and lignin concentration with partial removal of extractives and hemicelluloses. The subsequent enzymatic hydrolysis incurred further deacetylation of the xylan, leaving approximately 10 % of acetate intact based on the weight of original wheat straw.
BioEnergy Research arrow_drop_down University of Copenhagen: ResearchArticle . 2013Data sources: Bielefeld Academic Search Engine (BASE)Griffith University: Griffith Research OnlineArticle . 2013Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.eu70 citations 70 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert BioEnergy Research arrow_drop_down University of Copenhagen: ResearchArticle . 2013Data sources: Bielefeld Academic Search Engine (BASE)Griffith University: Griffith Research OnlineArticle . 2013Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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description Publicationkeyboard_double_arrow_right Article , Journal 2017 BelgiumPublisher:Oxford University Press (OUP) Funded by:EC | P-MEDICINEEC| P-MEDICINEWannes Voorend; Wout Boerjan; Dharshana Padmakshan; Dharshana Padmakshan; Andreas Pallidis; José Nicomedes Junior; José Nicomedes Junior; Geert Goeminne; Hoon Kim; Hoon Kim; Lívia Vargas; Jan Van Doorsselaere; Fernando Campos de Assis Fonseca; Marina de Lyra Soriano Saleme; John Ralph; John Ralph; Ruben Vanholme; Rebecca Van Acker; Igor Cesarino; Igor Cesarino;Caffeoyl shikimate esterase (CSE) was recently shown to play an essential role in lignin biosynthesis in Arabidopsis (Arabidopsis thaliana) and later in Medicago truncatula However, the general function of this enzyme was recently questioned by the apparent lack of CSE activity in lignifying tissues of different plant species. Here, we show that down-regulation of CSE in hybrid poplar (Populus tremula × Populus alba) resulted in up to 25% reduced lignin deposition, increased levels of p-hydroxyphenyl units in the lignin polymer, and a relatively higher cellulose content. The transgenic trees were morphologically indistinguishable from the wild type. Ultra-high-performance liquid chromatography-mass spectrometry-based phenolic profiling revealed a reduced abundance of several oligolignols containing guaiacyl and syringyl units and their corresponding hydroxycinnamaldehyde units, in agreement with the reduced flux toward coniferyl and sinapyl alcohol. These trees accumulated the CSE substrate caffeoyl shikimate along with other compounds belonging to the metabolic classes of benzenoids and hydroxycinnamates. Furthermore, the reduced lignin amount combined with the relative increase in cellulose content in the CSE down-regulated lines resulted in up to 62% more glucose released per plant upon limited saccharification when no pretreatment was applied and by up to 86% and 91% when acid and alkaline pretreatments were used. Our results show that CSE is not only important for the lignification process in poplar but is also a promising target for the development of improved lignocellulosic biomass crops for sugar platform biorefineries.
PLANT PHYSIOLOGY arrow_drop_down Ghent University Academic BibliographyArticle . 2017Data sources: Ghent University Academic Bibliographyadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess Routesbronze 96 citations 96 popularity Top 1% influence Top 10% impulse Top 1% Powered by BIP!
more_vert PLANT PHYSIOLOGY arrow_drop_down Ghent University Academic BibliographyArticle . 2017Data sources: Ghent University Academic Bibliographyadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1104/pp.17.00920&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article 2023 United StatesPublisher:Wiley Taichi E. Takasuka; Hoon Kim; Kai Deng; Christopher M. Bianchetti; Kaho Yamashita; Emily T. Beebe; Lai F. Bergeman; Kirk A. Vander Meulen; Samuel Deutsch; John Ralph; Paul D. Adams; Trent R. Northen; Brian G. Fox;pmid: 37402642
AbstractKelp is an abundant, farmable biomass‐containing laminarin and alginate as major polysaccharides, providing an excellent model substrate to study their deconstruction by simple enzyme mixtures. Our previous study showed strong reactivity of the glycoside hydrolase family 55 during hydrolysis of purified laminarin, raising the question of its reactivity with intact kelp. In this study, we determined that a combination of a single glycoside hydrolase family 55 β‐1,3‐exoglucanase with a broad‐specificity alginate lyase from the polysaccharide lyase family 18 gives efficient hydrolysis of untreated kelp to a mixture of simple sugars, that is, glucose, gentiobiose, mannitol‐end glucose, and mannuronic and guluronic acids and their soluble oligomers. Quantitative assignments from nanostructure initiator mass spectrometry (NIMS) and 2D HSQC NMR spectroscopy and analysis of the reaction time‐course are provided. The data suggest that binary combinations of enzymes targeted to the unique polysaccharide composition of marine biomass are sufficient to deconstruct kelp into soluble sugars for microbial fermentation.
University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2023License: CC BY NC NDFull-Text: https://escholarship.org/uc/item/8421v8cqData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2023Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess RoutesGreen hybrid 4 citations 4 popularity Top 10% influence Average impulse Average Powered by BIP!
more_vert University of Califo... arrow_drop_down University of California: eScholarshipArticle . 2023License: CC BY NC NDFull-Text: https://escholarship.org/uc/item/8421v8cqData sources: Bielefeld Academic Search Engine (BASE)eScholarship - University of CaliforniaArticle . 2023Data sources: eScholarship - University of Californiaadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1002/cbic.202300357&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type , Journal 2015 BelgiumPublisher:Springer Science and Business Media LLC Funded by:UKRI | RootDetect: Remote Detect..., NSF | Purchase of an Electrospr...UKRI| RootDetect: Remote Detection and Precision Management of Root Health ,NSF| Purchase of an Electrospray Ionization Mass SpectrometerFachuang Lu; Steven D. Karlen; Matt Regner; Hoon Kim; Sally A. Ralph; Run Cang Sun; Ken-ichi Kuroda; Mary Ann Augustin; Raymond Mawson; Henry Sabarez; Tanoj K. Singh; Gerardo Jimenez-Monteon; Sarani Zakaria; Stefan J. Hill; Philip J. Harris; Wout Boerjan; Curtis G. Wilkerson; Shawn D. Mansfield; John Ralph;handle: 1854/LU-8637689
La production industrielle d'huile de palme génère simultanément une quantité substantielle de fibres de grappe de fruits vides (EFB) qui pourraient être utilisées comme matière première dans une bioraffinerie à base de lignocellulose. Les sous-produits de la lignine générés par ce processus peuvent offrir des possibilités d'isolation des produits à valeur ajoutée, tels que le p-hydroxybenzoate (pBz), pour aider à compenser les coûts d'exploitation. L'analyse de la lignine EFB par spectroscopie de résonance magnétique nucléaire (RMN) a clairement révélé la présence d'acétate lié et de pBz, la saponification révélant que 1,1 % en poids de l'EFB était du pBz ; avec une teneur en lignine de 22,7 %, 4,8 % de la lignine est du pBz qui peut être obtenu comme composant pur pour être utilisé comme charge chimique. L'analyse de la lignine EFB par RMN et dérivatisation suivie d'un clivage réducteur (DFRC) a montré que le pBz acylate sélectivement le groupe γ-hydroxyle des unités S. Cette sélectivité suggère que le pBz, de manière analogue à l'acétate dans le kénaf, le p-coumarate dans les graminées et le ferrate dans un peuplier transgénique augmenté d'une féruloyl-CoA monolignol transférase (FMT), est incorporé dans la chaîne de lignine en croissance via son conjugué monolignol γ-p-hydroxybenzoylé. L'implication de tels conjugués dans la lignification des palmiers est prouvée par l'observation de nouvelles unités couplées au β-β non résinol p-hydroxybenzoylé dans les lignines. Ensemble, les données impliquent l'existence de p-hydroxybenzoyl-CoA :monolignol transférases qui sont impliquées dans la lignification chez les différents saules (Salix spp.), peupliers et peupliers faux-trembles (Populus spp., famille des Salicacées) et palmiers (famille des Arecacées) qui ont des lignines p-hydroxybenzoylées. Même sans augmenter les niveaux par la sélection ou le génie génétique, les « déchets » actuels de l'EFB d'huile de palme devraient être en mesure de générer un flux important d'acide p-hydroxybenzoïque qui offre des opportunités pour le développement de produits à valeur ajoutée dérivés de l'industrie du palmier à huile. La producción industrial de aceite de palma genera simultáneamente una cantidad sustancial de fibras de racimo de frutas vacías (EFB) que podrían utilizarse como materia prima en una biorrefinería a base de lignocelulosa. Los subproductos de lignina generados por este proceso pueden ofrecer oportunidades para el aislamiento de productos de valor agregado, como el p-hidroxibenzoato (pBz), para ayudar a compensar los costos operativos. El análisis de la lignina EFB por espectroscopía de resonancia magnética nuclear (RMN) reveló claramente la presencia de acetato unido y pBz, y la saponificación reveló que el 1,1% en peso del EFB era pBz; con un contenido de lignina del 22,7 %, el 4,8 % de la lignina es pBz que se puede obtener como un componente puro para su uso como materia prima química. El análisis de la lignina EFB por RMN y la derivatización seguida de escisión reductora (DFRC) mostró que pBz acila selectivamente el grupo γ-hidroxilo de las unidades S. Esta selectividad sugiere que pBz, análogamente con acetato en kenaf, p-cumarato en gramíneas y ferular en un álamo transgénico aumentado con una feruloil-CoA monolignol transferasa (FMT), se incorpora a la cadena de lignina en crecimiento a través de su conjugado de monolignol γ-p-hidroxibenzoilado. La participación de dichos conjugados en la lignificación de la palma se demuestra al observar nuevas unidades no acopladas a β-β-resinol p-hidroxibenzoiladas en las ligninas. Juntos, los datos implican la existencia de p-hidroxibenzoil-CoA:monolignol transferasas que participan en la lignificación en los diversos sauces (Salix spp.), álamos y álamo temblón (Populus spp., familia Salicaceae) y palmeras (familia Arecaceae) que tienen ligninas p-hidroxibenzoiladas. Incluso sin mejorar los niveles mediante mejoramiento o ingeniería genética, los 'desechos' actuales de aceite de palma EFB deberían ser capaces de generar una corriente considerable de ácido p-hidroxibenzoico que ofrezca oportunidades para el desarrollo de productos de valor agregado derivados de la industria de la palma aceitera. The industrial production of palm oil concurrently generates a substantial amount of empty fruit bunch (EFB) fibers that could be used as a feedstock in a lignocellulose-based biorefinery. Lignin byproducts generated by this process may offer opportunities for the isolation of value-added products, such as p-hydroxybenzoate (pBz), to help offset operating costs. Analysis of the EFB lignin by nuclear magnetic resonance (NMR) spectroscopy clearly revealed the presence of bound acetate and pBz, with saponification revealing that 1.1 wt% of the EFB was pBz; with a lignin content of 22.7 %, 4.8 % of the lignin is pBz that can be obtained as a pure component for use as a chemical feedstock. Analysis of EFB lignin by NMR and derivatization followed by reductive cleavage (DFRC) showed that pBz selectively acylates the γ-hydroxyl group of S units. This selectivity suggests that pBz, analogously with acetate in kenaf, p-coumarate in grasses, and ferulate in a transgenic poplar augmented with a feruloyl-CoA monolignol transferase (FMT), is incorporated into the growing lignin chain via its γ-p-hydroxybenzoylated monolignol conjugate. Involvement of such conjugates in palm lignification is proven by the observation of novel p-hydroxybenzoylated non-resinol β–β-coupled units in the lignins. Together, the data implicate the existence of p-hydroxybenzoyl-CoA:monolignol transferases that are involved in lignification in the various willows (Salix spp.), poplars and aspen (Populus spp., family Salicaceae), and palms (family Arecaceae) that have p-hydroxybenzoylated lignins. Even without enhancing the levels by breeding or genetic engineering, current palm oil EFB 'wastes' should be able to generate a sizeable stream of p-hydroxybenzoic acid that offers opportunities for the development of value-added products derived from the oil palm industry. ينتج الإنتاج الصناعي لزيت النخيل في الوقت نفسه كمية كبيرة من ألياف مجموعة الفاكهة الفارغة (EFB) التي يمكن استخدامها كمادة وسيطة في مصفاة حيوية تعتمد على اللجينوسليلوز. قد توفر المنتجات الثانوية لليجنين الناتجة عن هذه العملية فرصًا لعزل المنتجات ذات القيمة المضافة، مثل p - hydroxybenzoate (pBz)، للمساعدة في تعويض تكاليف التشغيل. كشف تحليل اللجنين EFB بواسطة مطياف الرنين المغناطيسي النووي (NMR) بوضوح عن وجود أسيتات مرتبطة و pBz، مع كشف التصبن أن 1.1 ٪ بالوزن من EFB كان pBz ؛ مع محتوى اللجنين بنسبة 22.7 ٪، 4.8 ٪ من اللجنين عبارة عن pBz يمكن الحصول عليه كمكون نقي للاستخدام كمادة وسيطة كيميائية. أظهر تحليل اللجنين EFB بواسطة NMR والاشتقاق متبوعًا بالانقسام الاختزالي (DFRC) أن pBz يعمل بشكل انتقائي على أسيتيل مجموعة γ - hydroxyl من وحدات S. تشير هذه الانتقائية إلى أن pBz، بالتناظر مع الأسيتات في الكناف، و p - comarate في الأعشاب، و ferulate في حور معدّل وراثيًا معززًا بـ feruloyl - CoA monolignol transferase (FMT)، يتم دمجه في سلسلة اللجنين المتنامية عبر γ - p - hydroxybenzoylated monolignol conjugate. ثبت تورط مثل هذه المترافقات في ترصيع النخيل من خلال ملاحظة وحدات p - hydroxybenzoylated غير الراتينول β - β المقترنة في اللجنين. معا، تشير البيانات إلى وجود p - hydroxybenzoyl - CoA: monolignol transferases التي تشارك في تبييض في الصفصاف المختلفة (Salix spp.)، الحور والحور (Populus spp.، عائلة Salicaceae)، والنخيل (عائلة Arecaceae) التي تحتوي على p - hydroxybenzoylated lignins. حتى من دون تعزيز المستويات عن طريق التكاثر أو الهندسة الوراثية، يجب أن تكون "نفايات" زيت النخيل الحالية قادرة على توليد تيار كبير من حمض p - hydroxybenzoic الذي يوفر فرصًا لتطوير منتجات ذات قيمة مضافة مشتقة من صناعة نخيل الزيت.
BioEnergy Research arrow_drop_down Ghent University Academic BibliographyArticle . 2015Data sources: Ghent University Academic Bibliographyadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess Routeshybrid 110 citations 110 popularity Top 1% influence Top 10% impulse Top 1% Powered by BIP!
more_vert BioEnergy Research arrow_drop_down Ghent University Academic BibliographyArticle . 2015Data sources: Ghent University Academic Bibliographyadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2015Publisher:Springer Science and Business Media LLC Funded by:NSF | Fundamental Understanding..., UKRI | RootDetect: Remote Detect...NSF| Fundamental Understanding of HDA Process: One-Step Conversion of Lignocellulosic Biomass to Furan-Based Precursors for Drop-in Liquid Fuel ,UKRI| RootDetect: Remote Detection and Precision Management of Root HealthTae Hyun Kim; Chang Geun Yoo; Xuejun Pan; Hoon Kim; Ali Azarpira; Kyeong Keun Oh; John Ralph; John Ralph; Fachuang Lu; Jun Seok Kim;AbstractPhysicochemical characteristics of corn stover pretreated by soaking in aqueous ammonia (SAA) and low-moisture anhydrous ammonia (LMAA) were compared and investigated. The glucan digestibility of the treated biomass reached 90 % (SAA) and 84 % (LMAA). The LMAA pretreatment enhanced the digestibility by cleaving cross-linkages between cell wall components, whereas the SAA pretreatment additionally improved the digestibility by efficiently removing a major portion of the lignin under mild reaction conditions without significant loss of carbohydrates. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), and gel permeation chromatography (GPC) revealed the structural and chemical transformations of lignin during the pretreatments. Both pretreatments effectively cleaved ferulate cell wall cross-linking that is associated with the recalcitrance of grass lignocellulosics toward enzymatic saccharification. Extracted lignin from SAA pretreatment was extensively depolymerized but retained “native” character, as evidenced by the retention of β-ether linkages.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.euAccess Routeshybrid 51 citations 51 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
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You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-015-9662-6&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2008Publisher:Springer Science and Business Media LLC Funded by:NSF | Development of 750 MHz Sp..., NSF | National Biological NMR F..., NIH | 900 MHz NMR Spectrometer ... +4 projectsNSF| Development of 750 MHz Spectroscopy at the National Magnetic Resonance Facility at Madison. ,NSF| National Biological NMR Facility at Madison ,NIH| 900 MHz NMR Spectrometer Biomolecular Investigations ,NIH| CONSOLE UPGRADES FOR NMR FACILITY ,NIH| WIDE-BORE 9.4 TESLA NMR SPECTROMETER FOR UW-MADISON ,NIH| MAINTENANCE OF NMRFAM SPECTROMETERS (MAGNET TESTING) ,UKRI| RootDetect: Remote Detection and Precision Management of Root HealthTakuya Akiyama; Hoon Kim; Hoon Kim; John Ralph; John Ralph;Although finely divided ball-milled whole cell walls do not completely dissolve in dimethylsulfoxide (DMSO), they readily swell producing a gel. Solution-state two-dimensional (2D) nuclear magnetic resonance (NMR) of this gel, produced directly in the NMR tube, provides an interpretable structural fingerprint of the polysaccharide and lignin components of the wall without actual solubilization, and without structural modification beyond that inflicted by the ball milling and ultrasonication steps. Since the cellulose is highly crystalline and difficult to swell, the component may be under-represented in the spectra. The method however provides a more rapid method for comparative structural evaluation of plant cell walls than is currently available. With the new potential for chemometric analysis using the 2D NMR fingerprint, this method may find application as a secondary screen for selecting biomass lines and for optimizing biomass processing and conversion efficiencies.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.eu268 citations 268 popularity Top 1% influence Top 1% impulse Top 10% Powered by BIP!
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You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2017 Belgium, FrancePublisher:Oxford University Press (OUP) Funded by:EC | MULTIBIOPRO, EC | ENERGYPOPLAREC| MULTIBIOPRO ,EC| ENERGYPOPLARCatherine Lapierre; Françoise Laurans; Wout Boerjan; Geert Goeminne; Frédéric Legée; Annabelle Déjardin; Gilles Pilate; Lennart Hoengenaert; Cliff E. Foster; Sandrien Desmet; Hoon Kim; Hoon Kim; Ruben Vanholme; Kris Morreel; Rebecca Van Acker; Nicholas Santoro; John Ralph;In the search for renewable energy sources, genetic engineering is a promising strategy to improve plant cell wall composition for biofuel and bioproducts generation. Lignin is a major factor determining saccharification efficiency and, therefore, is a prime target to engineer. Here, lignin content and composition were modified in poplar (Populus tremula × Populus alba) by specifically down-regulating CINNAMYL ALCOHOL DEHYDROGENASE1 (CAD1) by a hairpin-RNA-mediated silencing approach, which resulted in only 5% residual CAD1 transcript abundance. These transgenic lines showed no biomass penalty despite a 10% reduction in Klason lignin content and severe shifts in lignin composition. Nuclear magnetic resonance spectroscopy and thioacidolysis revealed a strong increase (up to 20-fold) in sinapaldehyde incorporation into lignin, whereas coniferaldehyde was not increased markedly. Accordingly, ultra-high-performance liquid chromatography-mass spectrometry-based phenolic profiling revealed a more than 24,000-fold accumulation of a newly identified compound made from 8-8 coupling of two sinapaldehyde radicals. However, no additional cinnamaldehyde coupling products could be detected in the CAD1-deficient poplars. Instead, the transgenic lines accumulated a range of hydroxycinnamate-derived metabolites, of which the most prominent accumulation (over 8,500-fold) was observed for a compound that was identified by purification and nuclear magnetic resonance as syringyl lactic acid hexoside. Our data suggest that, upon down-regulation of CAD1, coniferaldehyde is converted into ferulic acid and derivatives, whereas sinapaldehyde is either oxidatively coupled into S'(8-8)S' and lignin or converted to sinapic acid and derivatives. The most prominent sink of the increased flux to hydroxycinnamates is syringyl lactic acid hexoside. Furthermore, low-extent saccharification assays, under different pretreatment conditions, showed strongly increased glucose (up to +81%) and xylose (up to +153%) release, suggesting that down-regulating CAD1 is a promising strategy for improving lignocellulosic biomass for the sugar platform industry.
PLANT PHYSIOLOGY arrow_drop_down Ghent University Academic BibliographyArticle . 2017Data sources: Ghent University Academic BibliographyInstitut National de la Recherche Agronomique: ProdINRAArticle . 2017Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1104/pp.17.00834&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesbronze 102 citations 102 popularity Top 1% influence Top 10% impulse Top 1% Powered by BIP!
more_vert PLANT PHYSIOLOGY arrow_drop_down Ghent University Academic BibliographyArticle . 2017Data sources: Ghent University Academic BibliographyInstitut National de la Recherche Agronomique: ProdINRAArticle . 2017Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1104/pp.17.00834&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article 2023Publisher:Royal Society of Chemistry (RSC) Feng Cheng; Sarah Liu; Steven D. Karlen; Hoon Kim; Fachuang Lu; John Ralph; Leida M. Vázquez Ramos; George W. Huber; James A. Dumesic;doi: 10.1039/d2gc03446h
In this paper, we describe an approach for producing both high quality and high quantity of lignin by studying structural change of lignin during treatment of poplar wood in γ-valerolactone (GVL) for a range of temperatures (80–120 °C) and reaction time at temperature (1–24 h).
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1039/d2gc03446h&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu29 citations 29 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1039/d2gc03446h&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type 2024Publisher:Springer Science and Business Media LLC Funded by:UKRI | RootDetect: Remote Detect...UKRI| RootDetect: Remote Detection and Precision Management of Root HealthFaride Unda; Lisanne de Vries; Steven D. Karlen; Jordan Rainbow; Chengcheng Zhang; Laura E. Bartley; Hoon Kim; John Ralph; Shawn D. Mansfield;Abstract Background The phenolic polymer lignin is one of the primary chemical constituents of the plant secondary cell wall. Due to the inherent plasticity of lignin biosynthesis, several phenolic monomers have been shown to be incorporated into the polymer, as long as the monomer can undergo radicalization so it can participate in coupling reactions. In this study, we significantly enhance the level of incorporation of monolignol ferulate conjugates into the lignin polymer to improve the digestibility of lignocellulosic biomass. Results Overexpression of a rice Feruloyl-CoA Monolignol Transferase (FMT), OsFMT1, in hybrid poplar (Populus alba x grandidentata) produced transgenic trees clearly displaying increased cell wall-bound ester-linked ferulate, p-hydroxybenzoate, and p-coumarate, all of which are in the lignin cell wall fraction, as shown by NMR and DFRC. We also demonstrate the use of a novel UV–Vis spectroscopic technique to rapidly screen plants for the presence of both ferulate and p-hydroxybenzoate esters. Lastly we show, via saccharification assays, that the OsFMT1 transgenic p oplars have significantly improved processing efficiency compared to wild-type and Angelica sinensis-FMT-expressing poplars. Conclusions The findings demonstrate that OsFMT1 has a broad substrate specificity and a higher catalytic efficiency compared to the previously published FMT from Angelica sinensis (AsFMT). Importantly, enhanced wood processability makes OsFMT1 a promising gene to optimize the composition of lignocellulosic biomass.
Biotechnology for Bi... arrow_drop_down Biotechnology for Biofuels and BioproductsArticle . 2024 . Peer-reviewedLicense: CC BYData sources: Crossrefhttps://doi.org/10.21203/rs.3....Article . 2024 . Peer-reviewedLicense: CC BYData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1186/s13068-024-02544-y&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 1 citations 1 popularity Average influence Average impulse Average Powered by BIP!
more_vert Biotechnology for Bi... arrow_drop_down Biotechnology for Biofuels and BioproductsArticle . 2024 . Peer-reviewedLicense: CC BYData sources: Crossrefhttps://doi.org/10.21203/rs.3....Article . 2024 . Peer-reviewedLicense: CC BYData sources: Crossrefadd ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1186/s13068-024-02544-y&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Other literature type , Journal 2016Publisher:Springer Science and Business Media LLC Hoon Kim; Xuebin Zhang; Kewei Zhang; Yuanheng Cai; John Ralph; Chang-Jun Liu; Lisa M. Miller; Huijun Yang; Huan Feng; Huan Feng; Guichuan Hou;AbstractProducing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in the plant cell wall. Manipulating the monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs plant growth and development. Here, we show that expressing an engineered 4-O-methyltransferase that chemically modifies the phenolic moiety of lignin monomeric precursors, thus preventing their incorporation into the lignin polymer, substantially alters hybrid aspens’ lignin content and structure. Woody biomass derived from the transgenic aspens shows a 62% increase in the release of simple sugars and up to a 49% increase in the yield of ethanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation. Moreover, the cell wall structural changes do not affect growth and biomass production of the trees. Our study provides a useful strategy for tailoring woody biomass for bio-based applications.
add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1038/ncomms11989&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess RoutesGreen gold 64 citations 64 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1038/ncomms11989&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal 2012 Australia, DenmarkPublisher:Springer Science and Business Media LLC Kolby Hirth; Hoon Kim; John Ralph; Claus Felby; Prasad Kaparaju; Prasad Kaparaju; Daniel J. Yelle; Christopher G. Hunt;handle: 10072/67631
Solution-state two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy of plant cell walls is a powerful tool for characterizing changes in cell wall chemistry during the hydrothermal pretreatment process of wheat straw for second-generation bioethanol production. One-bond 13C–1H NMR correlation spectroscopy, via an heteronuclear single quantum coherence experiment, revealed substantial lignin β-aryl ether cleavage, deacetylation via cleavage of the natural acetates at the 2-O- and 3-O-positions of xylan, and uronic acid depletion via cleavage of the (1 → 2)-linked 4-O-methyl-α-d-glucuronic acid of xylan. In the polysaccharide anomeric region, decreases in the minor β-d-mannopyranosyl, and α-l-arabinofuranosyl units were observed in the NMR spectra from hydrothermally pretreated wheat straw. The aromatic region indicated only minor changes to the aromatic structures during the process (e.g., further deacylation revealed by the depletion in ferulate and p-coumarate structures). Supplementary chemical analyses showed that the hydrothermal pretreatment increased the cellulose and lignin concentration with partial removal of extractives and hemicelluloses. The subsequent enzymatic hydrolysis incurred further deacetylation of the xylan, leaving approximately 10 % of acetate intact based on the weight of original wheat straw.
BioEnergy Research arrow_drop_down University of Copenhagen: ResearchArticle . 2013Data sources: Bielefeld Academic Search Engine (BASE)Griffith University: Griffith Research OnlineArticle . 2013Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.1007/s12155-012-9247-6&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu70 citations 70 popularity Top 10% influence Top 10% impulse Top 10% Powered by BIP!
more_vert BioEnergy Research arrow_drop_down University of Copenhagen: ResearchArticle . 2013Data sources: Bielefeld Academic Search Engine (BASE)Griffith University: Griffith Research OnlineArticle . 2013Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
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For further information contact us at helpdesk@openaire.eu