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description Publicationkeyboard_double_arrow_right Article , Journal , Other literature type 2021 MalaysiaPublisher:MDPI AG Authors:Dalia Sukmawati;
Nurul Family; Iman Hidayat;Dalia Sukmawati
Dalia Sukmawati in OpenAIRER. Sayyed;
+5 AuthorsR. Sayyed
R. Sayyed in OpenAIREDalia Sukmawati;
Nurul Family; Iman Hidayat;Dalia Sukmawati
Dalia Sukmawati in OpenAIRER. Sayyed;
R. Sayyed
R. Sayyed in OpenAIREElsayed Elsayed;
Daniel Dailin;Elsayed Elsayed
Elsayed Elsayed in OpenAIRESiti Hanapi;
Mohammad Wadaan;Siti Hanapi
Siti Hanapi in OpenAIREHesham Enshasy;
Hesham Enshasy
Hesham Enshasy in OpenAIREdoi: 10.3390/su13137479
This study aimed to isolate and identify moulds from rotten Citrus sinensis post-harvests and to investigate the activity of antagonist and biocontrol activity moulds that cause citrus fruit rotting. A total of 12 mould isolates were obtained. Following the pathogenicity test, two representative mould isolates were selected and identified based on the sequence analyses of internal transcribed spacer (ITS) regions of the rDNA. Methods used in this study include isolation of fungal postharvest diseases, pathogenicity assay, antagonism assay, growth curve analysis, in vitro biocontrol assay, and molecular phylogenetic analysis. Two isolates of fungal postharvest diseases were determined as the most destructive pathogens. The biocontrol assay showed that isolates of Y1 and Y10 were capable to reduce the growth of fungal isolates K6 and K9 and mitigate up to 100% of the damage of sweet citrus fruits after 7 days of incubation. The moulds were identified as K6 (Aspergillus flavus sensu lato) and K9 (Aspergillus niger sensu lato). Phylogenetic analysis showed that the Y10 yeast isolate was identified as Candida orthopsilosis, whereas the Y1 isolate had a close genetic relationship with Aureobasidium pullulans and possibly belongs to a new species. Further analysis is necessary to confirm this finding.
Sustainability arrow_drop_down SustainabilityOther literature type . 2021License: CC BYFull-Text: http://www.mdpi.com/2071-1050/13/13/7479/pdfData sources: Multidisciplinary Digital Publishing InstituteUniversiti Teknologi Malaysia: Institutional RepositoryArticle . 2021Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su13137479&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 44 citations 44 popularity Top 10% influence Top 10% impulse Top 1% Powered by BIP!
more_vert Sustainability arrow_drop_down SustainabilityOther literature type . 2021License: CC BYFull-Text: http://www.mdpi.com/2071-1050/13/13/7479/pdfData sources: Multidisciplinary Digital Publishing InstituteUniversiti Teknologi Malaysia: Institutional RepositoryArticle . 2021Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su13137479&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eudescription Publicationkeyboard_double_arrow_right Article , Journal , Other literature type 2021 MalaysiaPublisher:MDPI AG Authors:Dalia Sukmawati;
Nurul Family; Iman Hidayat;Dalia Sukmawati
Dalia Sukmawati in OpenAIRER. Sayyed;
+5 AuthorsR. Sayyed
R. Sayyed in OpenAIREDalia Sukmawati;
Nurul Family; Iman Hidayat;Dalia Sukmawati
Dalia Sukmawati in OpenAIRER. Sayyed;
R. Sayyed
R. Sayyed in OpenAIREElsayed Elsayed;
Daniel Dailin;Elsayed Elsayed
Elsayed Elsayed in OpenAIRESiti Hanapi;
Mohammad Wadaan;Siti Hanapi
Siti Hanapi in OpenAIREHesham Enshasy;
Hesham Enshasy
Hesham Enshasy in OpenAIREdoi: 10.3390/su13137479
This study aimed to isolate and identify moulds from rotten Citrus sinensis post-harvests and to investigate the activity of antagonist and biocontrol activity moulds that cause citrus fruit rotting. A total of 12 mould isolates were obtained. Following the pathogenicity test, two representative mould isolates were selected and identified based on the sequence analyses of internal transcribed spacer (ITS) regions of the rDNA. Methods used in this study include isolation of fungal postharvest diseases, pathogenicity assay, antagonism assay, growth curve analysis, in vitro biocontrol assay, and molecular phylogenetic analysis. Two isolates of fungal postharvest diseases were determined as the most destructive pathogens. The biocontrol assay showed that isolates of Y1 and Y10 were capable to reduce the growth of fungal isolates K6 and K9 and mitigate up to 100% of the damage of sweet citrus fruits after 7 days of incubation. The moulds were identified as K6 (Aspergillus flavus sensu lato) and K9 (Aspergillus niger sensu lato). Phylogenetic analysis showed that the Y10 yeast isolate was identified as Candida orthopsilosis, whereas the Y1 isolate had a close genetic relationship with Aureobasidium pullulans and possibly belongs to a new species. Further analysis is necessary to confirm this finding.
Sustainability arrow_drop_down SustainabilityOther literature type . 2021License: CC BYFull-Text: http://www.mdpi.com/2071-1050/13/13/7479/pdfData sources: Multidisciplinary Digital Publishing InstituteUniversiti Teknologi Malaysia: Institutional RepositoryArticle . 2021Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su13137479&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.euAccess Routesgold 44 citations 44 popularity Top 10% influence Top 10% impulse Top 1% Powered by BIP!
more_vert Sustainability arrow_drop_down SustainabilityOther literature type . 2021License: CC BYFull-Text: http://www.mdpi.com/2071-1050/13/13/7479/pdfData sources: Multidisciplinary Digital Publishing InstituteUniversiti Teknologi Malaysia: Institutional RepositoryArticle . 2021Data sources: Bielefeld Academic Search Engine (BASE)add ClaimPlease grant OpenAIRE to access and update your ORCID works.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.This Research product is the result of merged Research products in OpenAIRE.
You have already added works in your ORCID record related to the merged Research product.All Research productsarrow_drop_down <script type="text/javascript"> <!-- document.write('<div id="oa_widget"></div>'); document.write('<script type="text/javascript" src="https://beta.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=10.3390/su13137479&type=result"></script>'); --> </script>
For further information contact us at helpdesk@openaire.eu