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Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil
descriptionPublicationkeyboard_double_arrow_right Article , Journal 2011Publisher:Springer Science and Business Media LLC
Authors: Xuebing Xu; Derya Kahveci;
doi: 10.1007/s10529-011-0671-z
pmid: 21695486
doi: 10.1007/s10529-011-0671-z
pmid: 21695486
Candida rugosa lipase (CRL) and Candida antarctica lipase A (CALA) with improved activity and selectivity were prepared for use in organic solvent media. CRL bioimprinted with fatty acids exhibited eightfold enhanced transesterification activity in hexane. Combination of bioimprinting and coating with lecithin or with immobilization did not improve the activity further. CALA was immobilized with and without bioimprinting, none of which improved the activity. All modified lipases were tested for selective ethanolysis of fish oil to concentrate omega-3 polyunsaturated fatty acids (PUFA). None of the preparations, except the immobilized ones catalysed ethanolysis. Immobilized CRL-catalyzed ethanolysis giving 27% (v/v) ethyl esters (EE) in 48 h, of which 43 mol% was oleic acid but no PUFA was detected in the EE fraction. Fatty acid selectivity of CALA was significantly improved by immobilization combined with bioimprinting, resulting in 5.5-fold lower omega-3 PUFA in EE.
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Biotechnology Letters
Article . 2011 . Peer-reviewed
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Article . 2012
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Biotechnology Letter...arrow_drop_down
Biotechnology Letters
Article . 2011 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
Biotechnology Letters
Article . 2012
Data sources: Europe PubMed Central
Biotechnology Letters
Journal
Data sources: Microsoft Academic Graph
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citeCite
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Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil
descriptionPublicationkeyboard_double_arrow_right Article , Journal 2011Publisher:Springer Science and Business Media LLC
Authors: Xuebing Xu; Derya Kahveci;
doi: 10.1007/s10529-011-0671-z
pmid: 21695486
doi: 10.1007/s10529-011-0671-z
pmid: 21695486
Candida rugosa lipase (CRL) and Candida antarctica lipase A (CALA) with improved activity and selectivity were prepared for use in organic solvent media. CRL bioimprinted with fatty acids exhibited eightfold enhanced transesterification activity in hexane. Combination of bioimprinting and coating with lecithin or with immobilization did not improve the activity further. CALA was immobilized with and without bioimprinting, none of which improved the activity. All modified lipases were tested for selective ethanolysis of fish oil to concentrate omega-3 polyunsaturated fatty acids (PUFA). None of the preparations, except the immobilized ones catalysed ethanolysis. Immobilized CRL-catalyzed ethanolysis giving 27% (v/v) ethyl esters (EE) in 48 h, of which 43 mol% was oleic acid but no PUFA was detected in the EE fraction. Fatty acid selectivity of CALA was significantly improved by immobilization combined with bioimprinting, resulting in 5.5-fold lower omega-3 PUFA in EE.
Biotechnology Letter...arrow_drop_down
Biotechnology Letters
Article . 2011 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
Biotechnology Letters
Article . 2012
Data sources: Europe PubMed Central
Biotechnology Letters
Journal
Data sources: Microsoft Academic Graph
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
This Research product is the result of merged Research products in OpenAIRE.

You have already added works in your ORCID record related to the merged Research product.
Select content type to embed
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For further information contact us at helpdesk@openaire.eu
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bronze
32
citations 32
popularity Top 10%
influence Top 10%
impulse Average
Powered by BIP!
more_vert
Biotechnology Letter...arrow_drop_down
Biotechnology Letters
Article . 2011 . Peer-reviewed
License: Springer TDM
Data sources: Crossref
Biotechnology Letters
Article . 2012
Data sources: Europe PubMed Central
Biotechnology Letters
Journal
Data sources: Microsoft Academic Graph
linkLink to
shareshareShare
citeCite
addClaim
Please grant OpenAIRE to access and update your ORCID works.
This Research product is the result of merged Research products in OpenAIRE.

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This Research product is the result of merged Research products in OpenAIRE.

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Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil

Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil

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Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil

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Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil