• Energy Research
• 6. Clean water close
• 12. Responsible consumption close
• Bioresource Technology close

Increasing energy demand and depleting fossil fuel sources have intensified the focus on biofuel production. Microalgae have emerged as a desirable source for biofuel production because of high biomass and lipid production from waste water source. In this study, five microalgae were isolated from effluents of Nisargruna biogas plants. These isolates were identified based on morphology and partial 18S and 23S rRNA gene sequences. Growth and lipid accumulation potential of these microalgae were investigated. One isolate, Chlorella sp. KMN3, accumulated high biomass (1.59 ± 0.05 g L(-1)) with moderate lipid content (20%), while another isolate Monoraphidium sp. KMN5 showed moderate biomass accumulation of 0.65 ± 0.05 g L(-1) with a very high (35%) lipid content. The fatty acid methyl esters mainly composed of C-16:0, C-18:0, C-18:1 and C-18:2. This observation makes these microalgae immensely potential candidate for biodiesel production using the effluent of a biogas plant as feed stock.

This article presents the application of microwave-induced pyrolysis to total recovery of resources and energy from rice straw. The microwave power and particle size of feedstock were both key parameters affecting the performance of microwave-induced pyrolysis. Under 400-500W microwave power, the reduction of fixed carbon in the biomass was significant. From the experimental results of specific surface area, zeta potential, and Cu2+ adsorption, the applications of solid residues in the water and wastewater treatment could be expected. The major compositions in gaseous product were H2, CO2, CO, CH4 of 55, 17, 13, 10vol.%, respectively. The high H2 content might imply that microwave-induced pyrolysis of biomass waste has the potential to produce the H2-rich fuel gas. Alkanes, polars, and low-ringed polycyclic aromatic hydrocarbons were three primary kinds of compounds in the liquid product.

The objective is to investigate the release characteristics of potassium (K) and chlorine (Cl) for torrefied wheat straw during a combined pyrolysis-combustion system. Powder and flake wheat straw samples were torrefied at different temperature of 200-300 ℃. The basic characteristics of torrefied samples and the K and Cl release rates of torrefied samples and their char samples derived from pyrolysis at 400-800 ℃ were analyzed and characterized. The results indicated that release rate of Cl was significantly higher than that of K under the same torrefaction conditions. In order to keep most of K and Cl remaining in biochar as well as the volatiles were completely released, the pyrolysis temperature of 700 ℃ for pyrolysis-combustion system was suitable. The total release rate of K and Cl at the pyrolysis temperature of 700 ℃ both exhibited a change trend of decreasing first and then increasing with the increase of torrefaction severity.

Microbial anodes were formed under polarization at -0.3 V/SCE on graphite plates in effluents from a pulp and paper mill. The bioanodes formed with the addition of acetate led to the highest current densities (up to 6A/m(2)) but were then unable to oxidize the raw effluent efficiently (0.5A/m(2)). In contrast, the bioanodes formed without acetate addition were fully able to oxidize the organic matter contained in the effluent, giving up to 4.5A/m(2) in continuous mode. Bacterial communities showed less bacterial diversity for the acetate-fed bioanodes compared to those formed in raw effluents. Deltaproteobacteria were the most abundant taxonomic group, with a high diversity for bioanodes formed without acetate addition but with almost 100% Desulfuromonas for the acetate-fed bioanodes. The addition of acetate to form the microbial anodes induced microbial selection, which was detrimental to the treatment of the raw effluent.

The effect of trace elements (TEs) addition and NaOH pretreatment on the anaerobic digestion of rice straw was investigated in batch tests. Co, Ni and Se were added to the raw rice straw at different dosages. The NaOH pretreatment was applied to the rice straw both alone and in combination with the addition of TEs, in order to evaluate potential synergistic effects of the pretreatment and the TEs supplementation on the biogas production yields. The results obtained showed that the alkaline pretreatment was more effective than the TEs addition in increasing the cumulative biogas production, causing a 21.4% enhancement of the final biomethane yield, whereas the increase due to TEs dosing was not statistically significant. The analysis of volatile fatty acids (VFAs) confirmed that the NaOH pretreatment resulted in a higher production of VFAs, indicating an increased hydrolysis, while TEs addition did not cause significant changes in the VFA concentrations.

As one of the most abundant biomass resources, crop stalks are great potential feedstock available for anaerobic digestion (AD) to produce biogas. However, the specific physical properties and complex chemical structures of crop stalks form strong barriers to efficient AD bioconversion. To overcome these problems, many efforts have been made over the past few years. This paper reviewed recent research in the evolving field of anaerobic bioconversion of crop stalks and was focused on three critical aspects affecting AD performance: various pretreatment methods and their effects on the improvement of crop stalk biodegradability, determination of specific AD operation parameters for crop stalks, and development of AD technologies. Finally, recommendations on the future development of crop stalk AD were proposed.

An anaerobic moving bed membrane bioreactor (AnMBMBR) fed with synthetic domestic wastewater was investigated under hydraulic retention time (HRT) shocks to assess the effects on the microbial (bacteria and archaea) community and reactor performance. 16S rDNA targeted polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) approach was optimized to relate the metabolic and community composition with biogas generation, methane content and COD removal efficiency. From the drastic decrease of HRT (from 8 h to 4 h), the methane production was significantly reduced due to the HRT shock, while the COD removal efficiency was not affected. The enhanced growth of homoacetogenic bacteria, Thermoanaerobacteraceae competes with methanogens under shock period. When the HRT was recovered to 8 h, the methane generation rate was higher than the initial operation before the shock HRT changes, which would be ascribed to the activity of new emerging hydrogenotrophic archaea, Methanocella sp. and Methanofollis sp.

To improve the lignocellulose conversion for ABE in high solids fermentation, this study explored the feasibility of cycling the process through the cellulolytic or/and solventogenic phases via intermittent flushing of the fermentation media. Five different flushing strategies (varying medium ingredients, inoculum supplement and cycling through phases) were investigated. Flushing regularly throughout the cellulolytic phase is necessary because re-incubation at 65 °C significantly improved glucose availability by at least 6-fold. The solvents accumulation was increased by 4-fold using corn stover (3-fold using miscanthus) over that produced by flushing only through the solventogenic phase. In addition, cycling process was simplified by re-incubating the flushed cellulolytic phase with no re-inoculation because the initial inoculum of Clostridiumthermocellum remained viable throughout sequential co-culture. This study served as the first proof of the cycling flush system applied in co-cultural SSC and the knowledge gained can be used to design a farm-scale flushing system.