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Methane (CH4) can be used as an alternative carbon source for denitrification with added oxygen (O2). However, the off-gas of denitrification reactors using a CH4-O2 mixture contains unused CH4 and O2 in proportions that make it unusable for fuel, carry explosion risks, and, if released into the atmosphere, contribute to the greenhouse effect. This study tested a novel reactor with a partition dividing the headspace completely and extending partly into the liquid layer. When CH4 and O2 were supplied separately to the liquid layer on opposite sides of the partition, the methane-dependent denitrification (MDD) activity was similar to that when the two gases were supplied as a mixture. In reactors with separate gas supplies, the off-gas from the CH4 supply side was high in CH4 and low in O2, and was usable for fuel, and that from the O2 supply side was very low in CH4, and might be released into the atmosphere. MDD activity increased with the O2 supply rate, and separate discharge of CH4 and O2 was maintained. The concentration of dissolved methane in the effluent was decreased by lowering the CH4/O2 supply ratio to 1.0 and drawing the effluent from the O2 supply side. This novel reactor enhances the safety of MDD, allows reuse of methane as fuel, and reduces methane leakage to the atmosphere.

Food waste generation and its consequent environmental impacts are increasing due to rapid urbanization, the global population, and associated food demand. Microbial fuel cells (MFCs) are a sustainable technology through which this food waste can be treated and used to produce bioelectricity. This study used two MFC configurations, a two-stage anaerobic up-flow leachate reactor MFC and a single-stage MFC, comparing the potential to treat solid fruit waste and fruit waste leachate. The two-stage MFC showed a higher potential to remove substrate at a shorter time compared to single-stage MFC. In 30 days, the two-stage anaerobic up-flow leachate reactor had a power density of 221 mW/m2. It was able to remove more total solids (by 95 %), volatile solids (by 70 %), total chemical oxygen demand (by 83 %), soluble chemical oxygen demand (by 87 %), and carbohydrates (by 33 %) compared to the single-stage MFC. However, the single-stage MFC showed higher coulombic efficiency (by 86.7 %) compared to the two-stage MFC. The efficiency of single-stage MFC improved by adding buffer and maintaining a neutral pH level of the substrate. The results of this study emphasize the importance of reactor design and demonstrate that MFC can be a viable technology to generate bioenergy from food waste.

To investigate the role of phosphorus in lipid production under nitrogen starvation conditions, five types of media possessing different nitrogen and phosphorus concentrations or their combination were prepared to culture Chlorella vulgaris. It was found that biomass production under nitrogen deficient condition with sufficient phosphorus supply was similar to that of the control (with sufficient nutrition), resulting in a maximum lipid productivity of 58.39 mg/L/day. Meanwhile, 31P NMR showed that phosphorus in the medium was transformed and accumulated as polyphosphate in cells. The uptake rate of phosphorus in cells was 3.8 times higher than the uptake rate of the control. This study demonstrates that phosphorus plays an important role in lipid production of C. vulgaris under nitrogen deficient conditions and implies a potential to combine phosphorus removal from wastewater with biodiesel production via microalgae.

An innovative cathodic algal biofilm microbial fuel cell equipped with a bioactive oxygen consuming unit (AB-OCU-MFC) was proposed for enhancing the leachate treatment containing biorefractory organic matters and high strength of ammonium nitrogen. The proposed AB-OCU-MFC performed better with regard to COD, NH4+-N, TN removals and algal biomass yield than standalone algal biofilm-MFC and control reactors. AB-OCU-MFC with OCU of 2 cm thickness removed more than 86% of COD, 89.4% of NH4+-N, 76.7% of TN and produced a maximum voltage of 0.39 V and biomass productivity of 1.23 g·L-1·d-1. The High-throughput sequencing of DNA showed a significant change in microbial community of reactors implemented with OCU, in which the ratio of exoelectrogenic bacteria of anode and denitrifying bacteria on cathode were significantly increased. The results obtained by cathodic algal biofilm MFC with low cost and bioactive barrier of OCU, would provide a new sight for practical application of MFC.

The purpose of this study was to contribute to filling the knowledge gap in public opinion and knowledge about forest and its certification in Japan, as well as to identify key elements and the possible role of public opinion within integrated bottom-up policies, bridging the sectors of forest, environment and energy. For the study 1930 questionnaires were disseminated in a small town in early 2007. Results from the statistical analysis indicated that forest was perceived as an ecosystem with a protective function against e.g. soil erosion or flooding, rather than a place that might serve for wood production and providing jobs. Forest certification and bioenergy from forest were identified as key elements for future integrated bottom-up policies that need to concentrate on facilitating the linkage between forestry and renewable energy as well as on promoting environmentally sound management and forest certification.

Information on vermicomposting with Metaphire posthuma is scanty. This paper, therefore, aims to evaluate the bioconversion efficiency of this species against Eiseniafetida. For comparative analysis, different combinations of municipal solid waste (MSW) and cow dung were used as substrates. The contents of total N and availability of P, K, and Fe increased significantly in both Metaphire and Eisenia systems which was accompanied by substantial reduction in pH and total organic C. Both species exhibited similar levels of urease activity and microbial respiration. Moreover, bioavailability of heavy metals (Pb, Zn, Mn, and Cu) was reduced substantially during vermicomposting, irrespective of the earthworm species. In contrast, each species was distinguished by the enhancement either in microbial biomass C and phosphatase activity (Eisenia) or in humification and fulvic/humic acid C (Metaphire). The overall results suggest that indigenous earthworm, M.posthuma could be utilized as a successful candidate for bioprocessing of toxic wastes.

Corn straw silage (CSS) is one of the organic solid residues available for biogas production. The aim of this study was to investigate the possibility and optimal controlling strategy for anaerobic digestion (AD) of CSS. Four leaching bed reactors (LBR) were operated at different pH. Maximum volatile fatty acids (VFAs) concentration of 19.34 g/L was reached at pH 8.0 with acetic and propionic acids as dominant VFAs. The subsequent microbial analysis indicated that abundant bacteria were Firmicutes, Bacteroidetes and Proteobacteria. UASB as methanogenic reactor was integrated with the LBR. The organic loading rate (OLR) could reach 8 g COD/L·d with effective conversion of VFAs. Acetotrophic Methanosaeta and hydrogenotrophic Methanobacterium played major roles in methanogenic process. In the whole process, the results showed that methane yield of 143.4 mL CH4/g volatile solid (VS) was obtained. pH and OLR controls in two-phase AD were feasible for methane production from CSS.

Four biofilm membrane bioreactors (Bf-MBRs) with various fixed carrier volumes (C:M) were operated in parallel to investigate the effect of attached-growth mode biomass involvement to the change of liquid-phase organics characteristics and membrane permeability, by comparing with conventional MBR. The experiments displayed that C:M and co-existence of biofilm with suspended solids in Bf-MBRs resulted in slight difference in pollutants removal effectiveness, and in rather distinct biomass properties and bacterial activities. The membrane permeability and specific resistance of bulk suspension of Bf-MBRs related closely with the liquid-phase organic substance, including soluble microbial products (SMP) and biopolymer cluster (BPC). Compared with conventional MBR, Bf-MBR with proper C:M had a low total biomass content and food-chain, where biofilm formation and its dominance affected liquid-phase organics, especially through reducing their content and minimizing strongly and weakly hydrophobic components with small molecular weight, and thus to mitigate membrane fouling significantly.

To determine the feasibility of microalgae-yeast mixed culture using the liquid digestate of dairy wastewater (LDDW) for biofuels and single cell protein (SCP) production, the cell growth, nutrient removal and outputs evaluation of the mono and mixed culture of Chlorella vulgaris and Yarrowia lipolytica in LDDW were investigated by adding glycerol as carbon source. The results showed that the mixed culture could enhance the biological utilization efficiency of nitrogen and phosphorus, and obtain higher yield of biomass (1.62 g/L), lipid (0.31 g/L), protein (0.51 g/L), and higher heating value (34.06 KJ/L). Compared with the mono culture of C. vulgaris, a decline of the transcription level in nitrate reductase and glutamine synthetase II genes in C. vulgaris was observed in the mixed culture when ammonia was sufficient. The results suggest the possibility of using the mixed culture for the efficient treatment of LDDW and resources recycling.