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Urea is the most common nitrogen (N) fertilizer in agriculture, due to its cheaper price and high N content. Although the reciprocal influence between NO3- and NH4+ nutrition are well known, urea (U) interactions with these N-inorganic forms are poorly studied. Here, the responses of two tomato genotypes to ammonium nitrate (AN), U alone or in combination were investigated. Significant differences in root and shoot biomass between genotypes were observed. Under AN+U supply, Linosa showed higher biomass compared to UC82, exhibiting also higher values for many root architectural traits. Linosa showed higher Nitrogen Uptake (NUpE) and Utilization Efficiency (NUtE) compared to UC82, under AN+U nutrition. Interestingly, Linosa exhibited also a significantly higher DUR3 transcript abundance. These results underline the beneficial effect of AN+U nutrition, highlighting new molecular and physiological strategies for selecting crops that can be used for more sustainable agriculture. The data suggest that translocation and utilization (NUtE) might be a more important component of NUE than uptake (NUpE) in tomato. Genetic variation could be a source for useful NUE traits in tomato; further experiments are needed to dissect the NUtE components that confer a higher ability to utilize N in Linosa.

These data were collected as part of a case study for the ipaast project. The aim of the survey was to produce datasets interoperable for applications in archaeology and precision agriculture. The OptRx® Crop Sensors (AgLeader Technology, Ames, IO, USA) measure the reflectance in the 630–685 nm (red), 695–750 nm (RE red edge) and 760–850 nm (NIR—Near InfraRed) wavebands. Using those wavebands, NDVI and NDRE indexes are calculated. NDVI and NDRE are vegetative indexes obtained from the red, red-edge and NIR wavebands with formulas 1 and 2: NDVI = NIR−REDNIR+RED ; NDRE= NIR−RENIR+RE The two index values range from -1 (bare ground or water) to 1 (highly vigorous vegetation). To collect data, the sensor was mounted on a ground vehicle, a Kubota B2420 tractor. The sensor was paired with a GNNS receiver, GPS 6500 from AgLeader Technology (Ames, IO, USA). The instrumentation was coupled with the hardware and the rough book (Panasonic ToughPad FG-Z1, Panasonic Core. It was possible to install the sensor facing the ground using a metal bracket positioned on the front of the tractor. The sensor was positioned 1.15 m from the ground, emitting a rectangular footprint of 1.14 m in length and 20cm in width. The data were collected every 30 cm in alternate rows. 12 rows in total were analysed, covering a surface of 1.07 ha. Data were processed on QGIS. First, the data was interpolated with the Inverse Distance Weighting (IDW) function. The function was set up with a distance coefficient P of 4, with 40 rows and 98 columns. A Gaussian filter with a standard deviation value of 2 and a range of research of 3 was subsequently applied to create a representative raster.

Datasets associated with Agostini, S., Houlbreque, F., Biscéré, T., Harvey, B. P., Heitzman, J. M., Takimoto, R., et al. (2020). Greater mitochondrial energy production provides resistance to ocean acidification in ‘winning’ hermatypic corals. Front. Mar. Sci. 7. doi:10.3389/fmars.2020.600836.

We studied the performance of the AZTI Marine Biotic Index AMBI manipulating input data collected from lagoonal ecosystems. Our data set consisted of macrofaunal abundance and biomass counts gathered at a variety of sites at which the disturbance status was known. Input data were also manipulated using a set of transformations of increasing severity. Biotic indices were calculated using raw and transformed abundance, biomass and production. Among the three categories of AMBI-based indices, medium transformation of data gave the highest correlation with pressures. However, increasing the severity of transformation generally resulted in a decrease of the correlation with environmental factors. The relative importance of ecological groups changed when using abundance or biomass, sometimes leading to an improved ecological status classification. Being biomass and production more ecologically relevant than abundance, using them to derive AMBI-based new indices seems intriguing, at least in lagoonal waters, where the community is naturally disturbed and dominated by opportunists.

ABSTRACT The kinetics and the metabolism of Bifidobacterium adolescentis MB 239 growing on galactooligosaccharides (GOS), lactose, galactose, and glucose were investigated. An unstructured unsegregated model for growth in batch cultures was developed, and kinetic parameters were calculated with a recursive algorithm. The growth rate and cellular yield were highest on galactose, followed by lactose and GOS, and were lowest on glucose. Lactate, acetate, and ethanol yields allowed the calculation of carbon fluxes toward fermentation products. Distributions between two- and three-carbon products were similar on all the carbohydrates (55 and 45%, respectively), but ethanol yields were different on glucose, GOS, lactose, and galactose, in decreasing order of production. Based on the stoichiometry of the fructose-6-phosphate shunt and on the carbon distribution among the products, the ATP yield was calculated. The highest yield was obtained on galactose, while the yields were 5, 8, and 25% lower on lactose, GOS, and glucose, respectively. Therefore, a correspondence among ethanol production, low ATP yields, and low biomass production was established, demonstrating that carbohydrate preferences may result from different distributions of carbon fluxes through the fermentative pathway. During the fermentation of a GOS mixture, substrate selectivity based on the degree of polymerization was exhibited, since lactose and the trisaccharide were the first to be consumed, while a delay was observed until longer oligosaccharides were utilized. Throughout the growth on both lactose and GOS, galactose accumulated in the cultural broth, suggesting that β(1-4) galactosides can be hydrolyzed before they are taken up.

AbstractBACKGROUND: Inappropriate utilisation of biosolids may adversely impact agrosystem productivity. Here, we address the response of wheat (Triticum durum) to different doses (0, 40, 100, 200 and 300 t ha−1) of either municipal solid waste (MSW) compost or sewage sludge in a greenhouse pot experiment. Plant growth, heavy metal uptake, and antioxidant activity were considered.RESULTS: Biomass production of treated plants was significantly enhanced at 40 t ha−1 and 100 t ha−1 of MSW compost (+48% and +78% relative to the control, respectively). At the same doses of sewage sludge, the increase was only 18%. Higher doses of both biosolids restricted significantly the plant growth, in concomitance with the significant accumulation of heavy metals (Ni2+, Pb2+, Cu2+ and Zn2+), especially in leaves. Leaf activities of antioxidant enzymes (ascorbate peroxidase, glutathione reductase, catalase and superoxide dismutase) were unchanged at 40 t ha−1 MSW compost or sewage sludge, but were significantly stimulated at higher doses (200–300 t ha−1), together with higher leaf concentration of reduced glutathione.CONCLUSION: This preliminary study suggests that a MSW supply at moderate doses (100 t ha−1) could be highly beneficial for wheat productivity. Copyright © 2010 Society of Chemical Industry

The European Union has set ambitious targets of raising the share of EU energy consumption produced from renewable resources from 20% by 2020 to 27% by 2030. The aim of this paper is to assess the role of woody biomass in renewable energy as gross final energy consumption in the European Union (the EU-28). The paper identifies leading and lagging countries in biomass development by focusing on their current biomass use and forecasts future perspectives. The research compares and evaluates the role of biomass in renewable energy in the EU-28 focusing on countries' potential resources and policy support. The study shows that all countries are making efforts to reach the 20% target in 2020 and exhibit a trend of increasing renewable energy as gross final energy consumption towards the new target of 2030. Solid biomass plays an important role in reaching the EU's renewable energy targets. The majority of the EU-28 countries are close to reaching their national renewable energy targets and show a very attractive biomass development. Unless energy consumption decreases however, some member states will face serious problems in reaching their renewable energy target in 2020. Following our analysis, the largest problems occur in those MS having a relative high-energy consumption pattern: France, Germany and the United Kingdom. It is unlikely that they can comply with expected renewable energy demand, unless they mobilize more woody biomass from their available domestic potential (France, Germany) or considerably increase their woody biomass imports (mostly wood pellets) from elsewhere (United Kingdom).

Sentinel-2 data have been used in various fields of human activity. In cultural heritage, their potential is still to be fully explored. This paper aims to illustrate how remote sensing and open source tools are useful for archaeological investigations. The whole issue revolves around the application of satellite (Sentinel-2) and accessory tools for the identification, knowledge and protection of the cultural heritage of two areas of southern Italy: Sant’Arsenio (SA) and Foggia (FG). Both study cases were selected for a specific reason: to demonstrate the usefulness of open data and software for research and preservation of cultural heritage, as in the case of urban sprawl, development of public works (gas- and oil-pipelines, etc.) or intensive use of land for agricultural purposes. The results obtained are relevant for the knowledge improvement and very useful to operate in the field of preventive archaeology, for the evaluation and management of risk, the planning of city-expansion or infrastructures that could damage the buried heritage.