• Energy Research
• Closed Access close
• Embargo close
• IT close
• ES close
• National Research Council close

We report the effect of ethanol on monooxygenase activities in primary hamster hepatocyte cultures maintained on collagen-coated dishes. The addition of 50mM ethanol to cell cultures both from control and ethanol pretreated animals almost completely maintained, at least for 72hr, the P4502E1-dependent aniline hydroxylase (AnH) activity and the 2E1 immunodetectable apoprotein content at the levels of the corresponding 4-hr plated hepatocytes. On the contrary, other P450-dependent monooxygenase activities, as assayed by testosterone hydroxylases, kept decreasing falling-after 72hr of culture-to the levels of the 4-hr plated hepatocytes. In both cases, in the absence of ethanol, a rapid decline of AnH activities and 2E1 apoprotein contents were also observed, attaining undetectable levels at 72hr. The hybridizable 2E1 mRNA also rapidly declined in both cultures, but such decline was not significantly altered by the presence of 50mM ethanol in the culture medium. Furthermore, we show that P4502E1 in the liver possesses a rapid degradation phase with a half-life of about 6hr. Thus, in the hamster, P4502E1 appears regulated at post-translational level, as in rat, probably by a protein stabilization mechanism.

We report the effect of ethanol on monooxygenase activities in primary hamster hepatocyte cultures maintained on collagen-coated dishes. The addition of 50mM ethanol to cell cultures both from control and ethanol pretreated animals almost completely maintained, at least for 72hr, the P4502E1-dependent aniline hydroxylase (AnH) activity and the 2E1 immunodetectable apoprotein content at the levels of the corresponding 4-hr plated hepatocytes. On the contrary, other P450-dependent monooxygenase activities, as assayed by testosterone hydroxylases, kept decreasing falling-after 72hr of culture-to the levels of the 4-hr plated hepatocytes. In both cases, in the absence of ethanol, a rapid decline of AnH activities and 2E1 apoprotein contents were also observed, attaining undetectable levels at 72hr. The hybridizable 2E1 mRNA also rapidly declined in both cultures, but such decline was not significantly altered by the presence of 50mM ethanol in the culture medium. Furthermore, we show that P4502E1 in the liver possesses a rapid degradation phase with a half-life of about 6hr. Thus, in the hamster, P4502E1 appears regulated at post-translational level, as in rat, probably by a protein stabilization mechanism.

The effect of external EGR on knock was evaluated using a CFR engine. Combustion pressure was sampled on a time basis. A band pass filter in the time domain was applied to the pressure cycles. Five knock indices were calculated for each combustion cycle. The problem to quantify knock intensity was focused. At this extent, measurements were carried out on standard iso-octane-n-heptane blends in the test conditions used for the determ of the Motor Method Octane Number (MON). Knock intensity was varied acting on compression ratio. For each index, the conditions of absence of knock were determined using motored cycles. The indices were compared and one of them, showing the lowest C.O.V., was selected for further measurements. The effect of EGR on test fuels having different composition was evaluated varying the compression ratio, at fixed ignition timing. In this way, the same level of detonation, obtained in the absence of EGR, was realized with different amounts of external EGR. Percent variation of compression ratio was used to compare the ability of fuels, having different octane number, to tolerate compression ratio increase in presence of EGR. In particular, the tests were carried out on a matrix of twelve fuels with three levels of EGR. The results show that with all tested fuels the percent increase of compression ratio is strongly dependent on EGR.

The effect of external EGR on knock was evaluated using a CFR engine. Combustion pressure was sampled on a time basis. A band pass filter in the time domain was applied to the pressure cycles. Five knock indices were calculated for each combustion cycle. The problem to quantify knock intensity was focused. At this extent, measurements were carried out on standard iso-octane-n-heptane blends in the test conditions used for the determ of the Motor Method Octane Number (MON). Knock intensity was varied acting on compression ratio. For each index, the conditions of absence of knock were determined using motored cycles. The indices were compared and one of them, showing the lowest C.O.V., was selected for further measurements. The effect of EGR on test fuels having different composition was evaluated varying the compression ratio, at fixed ignition timing. In this way, the same level of detonation, obtained in the absence of EGR, was realized with different amounts of external EGR. Percent variation of compression ratio was used to compare the ability of fuels, having different octane number, to tolerate compression ratio increase in presence of EGR. In particular, the tests were carried out on a matrix of twelve fuels with three levels of EGR. The results show that with all tested fuels the percent increase of compression ratio is strongly dependent on EGR.

In the present work, an experimental study on a lab-scale adsorption refrigerator, based on activated carbon/ethanol working pair is reported. An extensive testing campaign has been carried out at the CNR ITAE laboratory, with multiple aims. First, the performance has been evaluated in terms of both COP and Specific Cooling Power (SCP), under different boundary conditions, including both air conditioning and refrigeration applications. Attractive SCPs, up to 180 W/kg and 70 W/kg for air conditioning and refrigeration, respectively, were measured. Under the same conditions, COP between 0.17 and 0.08 were obtained. In addition, different management strategies, namely, heat recovery between adsorbers and re-allocation of phase durations, were evaluated to identify their influence on the system. Both strategies confirmed the possibility of increasing COP and SCP up to 40% and 25%, respectively. Moreover, a design analysis based on the experimental results has been carried out, to suggest possible improvements of the system. The obtained results demonstrated the possibility of employing a non-toxic refrigerant like ethanol reaching performance comparable with other harmful refrigerants like ammonia and methanol.

In the present work, an experimental study on a lab-scale adsorption refrigerator, based on activated carbon/ethanol working pair is reported. An extensive testing campaign has been carried out at the CNR ITAE laboratory, with multiple aims. First, the performance has been evaluated in terms of both COP and Specific Cooling Power (SCP), under different boundary conditions, including both air conditioning and refrigeration applications. Attractive SCPs, up to 180 W/kg and 70 W/kg for air conditioning and refrigeration, respectively, were measured. Under the same conditions, COP between 0.17 and 0.08 were obtained. In addition, different management strategies, namely, heat recovery between adsorbers and re-allocation of phase durations, were evaluated to identify their influence on the system. Both strategies confirmed the possibility of increasing COP and SCP up to 40% and 25%, respectively. Moreover, a design analysis based on the experimental results has been carried out, to suggest possible improvements of the system. The obtained results demonstrated the possibility of employing a non-toxic refrigerant like ethanol reaching performance comparable with other harmful refrigerants like ammonia and methanol.

The official involvement of Italy in Antarctic research dates back to 1985, when Mario Zucchelli Station (the former Terra Nova Bay Station) was established in Terra Nova Bay. Italy joined the Antarctic Treaty in 1987. This article is an overview of the wide-ranging research in marine biology performed in the last three decades by the author's team in the Ross Sea. Fundamental questions have been addressed, related to cold adaptations--with special attention to the molecular bases--evolved by marine organisms along with progressive cooling in this geographic area, also analysed in comparison with other important areas, such as the Peninsula, the Weddell Sea, the sub-Antarctic and the Arctic. The basic stepping stone of this research was the integration of ecophysiology with molecular aspects, in the general framework of biodiversity, adaptation and evolution. Investigations have addressed a number of Ross Sea taxa, comprising fish, birds, urchins, whales, seals and bacteria. Its significance has special meaning in view of the control that Antarctica exerts on the world climate and ocean circulation, which has awakened great interest in the evolutionary biology of the organisms that live there.

The official involvement of Italy in Antarctic research dates back to 1985, when Mario Zucchelli Station (the former Terra Nova Bay Station) was established in Terra Nova Bay. Italy joined the Antarctic Treaty in 1987. This article is an overview of the wide-ranging research in marine biology performed in the last three decades by the author's team in the Ross Sea. Fundamental questions have been addressed, related to cold adaptations--with special attention to the molecular bases--evolved by marine organisms along with progressive cooling in this geographic area, also analysed in comparison with other important areas, such as the Peninsula, the Weddell Sea, the sub-Antarctic and the Arctic. The basic stepping stone of this research was the integration of ecophysiology with molecular aspects, in the general framework of biodiversity, adaptation and evolution. Investigations have addressed a number of Ross Sea taxa, comprising fish, birds, urchins, whales, seals and bacteria. Its significance has special meaning in view of the control that Antarctica exerts on the world climate and ocean circulation, which has awakened great interest in the evolutionary biology of the organisms that live there.