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Urea is the most common nitrogen (N) fertilizer in agriculture, due to its cheaper price and high N content. Although the reciprocal influence between NO3- and NH4+ nutrition are well known, urea (U) interactions with these N-inorganic forms are poorly studied. Here, the responses of two tomato genotypes to ammonium nitrate (AN), U alone or in combination were investigated. Significant differences in root and shoot biomass between genotypes were observed. Under AN+U supply, Linosa showed higher biomass compared to UC82, exhibiting also higher values for many root architectural traits. Linosa showed higher Nitrogen Uptake (NUpE) and Utilization Efficiency (NUtE) compared to UC82, under AN+U nutrition. Interestingly, Linosa exhibited also a significantly higher DUR3 transcript abundance. These results underline the beneficial effect of AN+U nutrition, highlighting new molecular and physiological strategies for selecting crops that can be used for more sustainable agriculture. The data suggest that translocation and utilization (NUtE) might be a more important component of NUE than uptake (NUpE) in tomato. Genetic variation could be a source for useful NUE traits in tomato; further experiments are needed to dissect the NUtE components that confer a higher ability to utilize N in Linosa.

This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No. 723649 Indoor environmental quality (IEQ) measurement and survey data of demonstration buildings in the hybridGEOTABS project

CK2 is a highly conserved protein kinase involved in different cellular processes, which shows a higher activity in actively proliferating mammalian cells and in various types of cancer and cancer cell lines. We recently demonstrated that CK2 activity is strongly influenced by growth rate in yeast cells as well. Here, we extend our previous findings and show that, in cells grown in either glucose or ethanol-supplemented media, CK2 presents no alteration in K(m) for both the ATP and the peptide substrate RRRADDSDDDDD, while a significant increase in V (max) is observed. In chemostat-grown cells, no difference of CK2 activity was observed in cells grown at the same dilution rate in media supplemented with either ethanol or glucose, excluding the contribution of carbon metabolism on CK2 activity. By using the eIF2β-derived peptide, which can be phosphorylated by the holoenzyme but not by the free catalytic subunits, we show that the holoenzyme activity requires the concurrent presence of both β and β' encoding genes. Finally, conditions of nitrogen deprivation leading to a G0-like arrest result in a decrease of total CK2 activity, but have no effect on the activity of the holoenzyme. These findings newly indicate a regulatory role of β and β' subunits of CK2 in the nutrient response.

AbstractThe co‐occurrence of chronic pain and alcohol use disorders (AUDs) involves complex interactions between genetic and neurophysiological aspects, and the research has reported mixed findings when they both co‐occur. There is also an indication of a gender‐dependent effect; males are more likely to use alcohol to cope with chronic pain problems than females. Recently, a new conceptualization has emerged, proposing that the negative affective component of pain drives and maintains alcohol‐related behaviors. We studied in a longitudinal fashion alterations in alcohol drinking patterns and pain thresholds in a mouse model of chronic neuropathic pain in a sex‐dependent manner. Following partial denervation (spared nerve injury [SNI]), stimulus‐evoked pain responses were measured before chronic alcohol consumption, during drinking, during a deprivation phase, and following an episode of excessive drinking. During the course of alcohol drinking, we observed pronounced sex differences in pain thresholds. Male mice showed a strong increase in pain thresholds, suggesting an analgesic effect induced by alcohol over time, an effect that was not observed in female mice. SNI mice did not differ from sham‐operated controls in baseline alcohol consumption. However, following a deprivation phase and the reintroduction of ethanol, male SNI mice but not female mice showed more pronounced excessive drinking than controls. Finally, we observed decreased central ethanol sensitivity in male SNI mice but not in females. Together with our finding, that ethanol is able to decrease a pain‐induced negative affective memory we come to following conclusion. We propose that a lower sensitivity to the intoxicating effects of alcohol together with the ability of alcohol to reduce the negative affective component of pain may explain the higher co‐occurrence of AUD in male chronic pain patients.

Berlin (Germany) has one of the longest climate records in the world (Cubasch and Kadow, 2011). In the 17th century mainly the family of the astronomer Kirch started measuring the temperature and wrote down general weather patterns. In the beginning of the 18th century the measurements became more regular including multiple measures per day. Over time, more inner city stations appeared but also disappeared. Therefore, this Berlin Climate Record is a moving station, but representable for the inner city of Berlin. With these datasets we reactivate the long inner city climate record in several variables. In this study, we digitized, analyzed, corrected, reconstructed, and provide the datasets on the very rare daily time frequency.

The Soutpansberg Transect investigates the abundance and diversity of ants and spiders along an altitudinal gradient in the Western Soutpansberg Mountains, South Africa. It has a north-south orientation over the mountain and is a long term project initiated in 2009. Data collection is carried out in intervals that suit the data logger capacity. To study the diversity patterns in ants and other invertebrate groups across the spatial transect over a long-term period and the climate the invertebrates.

Qualitative data set, containing formal and informal interviews field notes from non-participant observations archival documents from a case study of sustainability implementation (spec. living wages, BSCI, DETOX) along a footwear value chain between Germany and China. Data was collected with four parties of the value chain between 2015 and 2020. Languages: German, English, Mandarin (interpreted) Collected and analyzed for the dissertation (doc. rer. pol.) by Stephanie Schrage at Universität Hamburg (defended in October 2020)