Nowadays the use of cooling engine with refrigerant as its cooling source used by most people, but the use of these refrigerants such as CFCs and HFCs can cause thinning of the ozone layer in the atmosphere and also cause the greenhouse effect. Thermoelectric coolers can be used as a main source of alternative coolers that people needed. One example is the use of cooling box. The purpose of this study was to examine and analyze the cooling load in the cooling box that uses thermoelectric with energy came from photovoltaic. The study was done by using an experimental method in which the cooling box that has been designed, tested to determine the temperature inside the box by using a thermocouple. Other parameters such as Relative Humidity, Intensity of solar radiation, Dew-point Temperature were measured using HOBO data logger. Parameters that have been obtained then processed and calculated using mathematical equations to obtain the cooling load inside the cooling box. This study focused on the calculation of the efficiency of the photovoltaic, the calculation of the coefficient of performance of thermoelectric, the calculation of the capacity of battery to store energy, and analyze the cooling load. The analysis showed that the average cooling load in the cooling box with five 240 ml mineral water is 126,94 Watt, efficiency of photovoltaic is 15,57%, coefficient of performance of thermoelectric is 0,754 and the coefficient of performance of the system in cooling box is 0,225 100401033