Today’s world faces two major crises including environmental pollution due to fossil fuels and the reduction of these fuels, so energy supply through renewable energies has been considered a sustainable solution. The purpose of this study was to assess factors affecting the tendency to use biofuels among villagers. Heads of households in rural areas in Chavarzagh district, Tarom township, Zanjan province formed the statistical population of the study (N=2742). The sample size was estimated using the Cochrane formula (n=161) and samples were selected using multi-stage cluster sampling method. The descriptive and correlational research method was used in this study. The validity of the questionnaire was approved by a panel of related faculty members. Cronbach's alpha coefficient was used to confirm the reliability of the questionnaire (0.70-0.83). Data was analyzed using SPSS 21. Regression analysis showed that risk, attitudes towards biofuels, self-efficacy, training, eliminating subsidies for fossil fuels and encouraging the use of biofuels, increasing public awareness about the benefits of biofuels and perceived barriers explained 40.6 percent of the variation of villagers’ tendency to use biofuels.