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Journal of Chemical Technology & Biotechnology
Article . 2010 . Peer-reviewed
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An approach to optimization of enzymatic hydrolysis from sugarcane bagasse based on organosolv pretreatment

Authors: Mesa, Leyanis; González, Erenio; Cara, Cristóbal; Ruiz, Encarnación; Castro, Eulogio; Mussatto, Solange I.;

An approach to optimization of enzymatic hydrolysis from sugarcane bagasse based on organosolv pretreatment

Abstract

AbstractBACKGROUND: The organosolv pretreatment followed by enzymatic hydrolysis of the pretreated material and subsequent fermentation of the hydrolysate produced, was the strategy used for ethanol production from sugarcane bagasse. The effect of different operational variables affecting the pretreatment (the catalyst type and its concentration, and the pretreatment time) and enzymatic hydrolysis stage (substrate concentration, cellulase loading, addition of xylanase and Tween 20, and the cellulase/β‐glucosidase ratio), were investigated.RESULTS: The best values of glucose concentration (28.8 g L−1) and yield (25.1 g per 100 g dry matter) were obtained when the material was pretreated with 1.25% (w/w) H2SO4 for 60 min, and subsequently hydrolyzed using 10% (w/v) substrate concentration in a reaction medium supplemented with xylanase (300 UI g−1) and Tween 20 (2.5% w/w). Fermentation of the broth obtained under these optimum conditions by Saccharomyces cerevisiae resulted in an ethanol yield of 92.8% based on the theoretical yield, after 24 h.CONCLUSION: Organosolv pretreatment of sugarcane bagasse under soft conditions, and subsequent enzymatic hydrolysis of the pretreated material with a cellulolytic system supplemented with xylanase and Tween 20, is a suitable procedure to obtain a glucose rich hydrolysate efficiently fermentable to ethanol by Sacharomyces cerevisiae yeasts. Copyright © 2010 Society of Chemical Industry

Country
Portugal
Keywords

Science & Technology, Ethanol, Sugarcane bagasse, Saccharomyces cerevisiae, Enzymatic hydrolysis, Fermentation, fermentation, Saccharomyces cerevisiae, ethanol, organosolv pretreatment, enzymatic hydrolysis, Organosolv pretreatment

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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