AbstractWe have a poor understanding of how urban drainage and other engineered components interact with more natural hydrological processes in green and blue spaces to generate stream flow. This limits the scientific evidence base for predicting and mitigating the effects of future development of the built environment and climate change on urban water resources and their ecosystem services. Here, we synthesize > 20 years of environmental monitoring data to better understand the hydrological function of the 109-km2 Wuhle catchment, an important tributary of the river Spree in Berlin, Germany. More than half (56%) of the catchment is urbanized, leading to substantial flow path alterations. Young water from storm runoff and rapid subsurface flow provided around 20% of stream flow. However, most of it was generated by older groundwater (several years old), mainly recharged through the rural headwaters and non-urban green spaces. Recent drought years since 2018 showed that this base flow component has reduced in response to decreased recharge, causing deterioration in water quality and sections of the stream network to dry out. Attempts to integrate the understanding of engineered and natural processes in a traditional rainfall-runoff model were only partly successful due to uncertainties over the catchment area, effects of sustainable urban drainage, adjacent groundwater pumping, and limited conceptualization of groundwater storage dynamics. The study highlights the need for more extensive and coordinated monitoring and data collection in complex urban catchments and the use of these data in more advanced models of urban hydrology to enhance management.