Due to fluctuation in fuel prices in recent times the Indian economy is facing a heavy burden on the economic front to meet the energy supply demand. Due to large gap between the supply and demand of petroleum products. The government of India in its National Biofuel Policy is moving towards the development of biofuel as alternative source of energy by increasing its blends to 20 % by year 2017. In this path of development Karanja oil is being identified as second most important source of biodiesel production in India after Jatropha. But the poor stability and cold flow property associated with the Karanja biodiesel make it difficult for using it as alternative fuel to diesel. The storage of biodiesel is the major issue area for its long term utilization. The aim of present investigation is to optimize the storage period of Karanja biodiesel using various metals and antioxidants for a period of 6 months. The optimum result for OS of KB with same time and same amount of antioxidant for iron (Fe), aluminium (Al) and zinc (Zn) are 8.34, 8.40 and 8.20 h respectively with metal concentration level of 1.10, 0.85 and 1.45 ppm.