Ethanol-induced alteration in activities of cerebral phosphatidylinositol 4,5-biphosphate-specific and cytosolic phospholipase C in the brain: analysis using NG 108-15 cells and brains from ethanol-inhaled mice
Copyright policy )Ethanol-induced alteration in activities of cerebral phosphatidylinositol 4,5-biphosphate-specific and cytosolic phospholipase C in the brain: analysis using NG 108-15 cells and brains from ethanol-inhaled mice
Effect of long-term exposure to ethanol (EtOH) on the phosphatidylinositol 4,5-biphosphate (PIP2)-specific and cytosolic phospholipase C (PLC) activities in neuroblastoma x glioma hybrid (NG 108-15) cells and the brains from EtOH-inhaled mice were investigated. Long-term (2 days) exposure of NG 108-15 cells to EtOH induced significant decrease in PIP2-specific PLC activity dependent on concentration and duration of exposure, although the presence of EtOH in the enzyme assay system induced no alteration in PIP2-specific PLC activity. On the other hand, cytosolic PLC activity in NG 108-15 cells significantly increased by both the long-term exposure of the cells to EtOH and the addition of EtOH into the assay system. These changes in activities of both types of PLC in NG 108-15 cells observed after EtOH exposure recovered rapidly by the removal of EtOH. Moreover, the changes in activities of PIP2-specific and cytosolic PLC in the brain of EtOH-inhaled mice were similar to those found in NG 108-15 cells. These results indicate that EtOH inhibits the activity of PIP2-specific PLC and activates cytosolic PLC in the brain. These changes in cerebral PLC activities are suggested to involve in central action of EtOH and establishment of alcohol dependence.
Male, Phosphatidylinositol 4,5-Diphosphate, Dose-Response Relationship, Drug, Ethanol, Brain, Mice, Inbred Strains, Glioma, Hybrid Cells, Rats, Kinetics, Mice, Neuroblastoma, Cytosol, Phosphatidylinositol Phosphates, Type C Phospholipases, Administration, Inhalation, Tumor Cells, Cultured, Animals
Male, Phosphatidylinositol 4,5-Diphosphate, Dose-Response Relationship, Drug, Ethanol, Brain, Mice, Inbred Strains, Glioma, Hybrid Cells, Rats, Kinetics, Mice, Neuroblastoma, Cytosol, Phosphatidylinositol Phosphates, Type C Phospholipases, Administration, Inhalation, Tumor Cells, Cultured, Animals
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