Vineyards cover about eight millions of hectares worldwide and their annual pruning generates a large amount of ligno-cellulosic biomass, potentially available for industrial and energy use. Commercial pruning residue harvesters are now available, which may allow cost-effective recovery. The study aimed at determining the quantity and the quality of pruning residues potentially derived from vineyard management. Data were obtained from 17 fields in Northern and Central Italy. Fields were harvested with seven different machines. The experimental design adapted to the necessities of field trials, but was adequate for testing the main sources of variability. Net residue yield varies around 1 oven dry tonne per hectare, with minor differences between grape varietals and harvesting technologies. Losses are still high, and are generally related to ineffective management techniques. Moisture content at harvest varies between 40% and 45%, whereas the higher heating value is slightly lower than that of forest fuels. Comminuted vineyard residues are unsuitable for firing residential boilers, due to the frequent presence of oversize and/or undersize particles. The application of pesticides does not result in any significant contamination with noxious chemicals, because these products are almost completely weathered before residues are recovered. In wine-producing regions, the recovery of vineyard pruning residue may represent a substantial source of industrial bio-fuel. © 2011 Elsevier Ltd.