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Characterization and fatty acid profiling in two fresh water microalgae for biodiesel production: Lipid enhancement methods and media optimization using response surface methodology

descriptionPublicationkeyboard_double_arrow_right Article , Journal 01 Jul 2015Publisher:Elsevier BVJournal:Bioresource Technology, volume 188, pages 177-184 (issn: 0960-8524,

Authors: Kalimuthu Jawahar Raj; Rathinasamy Karpagam; Perumal Varalakshmi; Balasubramaniem Ashokkumar;

doi: 10.1016/j.biortech.2015.01.053

pmid: 25682476

Characterization and fatty acid profiling in two fresh water microalgae for biodiesel production: Lipid enhancement methods and media optimization using response surface methodology

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Abstract

Two fresh water microalgae, Coelastrella sp. M-60 and Micractinium sp. M-13 were investigated in this study for their potential of biodiesel production. For increasing biomass and lipid production, these microalgae were subjected to nutrient starvation (nitrogen, phosphorous, iron), salinity stress and nutrient supplementation with sugarcane industry effluent, citric acid, glucose and vitamin B12. The lipid productivity obtained from the isolates Coelastrella sp. M-60 (13.9 ± 0.4 mg/L/day) and Micractinium sp. M-13 (11.1 ± 0.2 mg/L/day) was maximum in salinity stress. The media supplemented with all the four nutrients yielded higher lipid productivity than the control. The response surface methodology (RSM) was employed to evaluate the effect of sugarcane industry effluent and citric acid on growth and lipid yield. Fatty acid profile of Coelastrella sp. M-60 and Micractinium sp. M-13 were composed of C-14, C-16:0, C-18:0, C-18:1 and C-18:2 and their fuel properties were also in accordance with international standards.

Related Organizations

Madurai Kamaraj University
India
Madurai Kamaraj University
India

Keywords

Osmosis, Salinity, Nitrogen, Iron, Fresh Water, Citric Acid, Industrial Microbiology, Microalgae, Biomass, Fatty Acids, Phosphorus, Lipids, Vitamin B 12, Glucose, Biofuels, Chromatography, Thin Layer, Gasoline

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