The current work describes the synthesis of Nano magnetic catalysts, oil extraction from fish waste and processing of biodiesel. The first-order thermodynamic equations are considered to determine its kinetic and thermodynamic parameters involved in extraction process. The study has considered the rate constant (k), activation energy (Ea), frequency factor (A), entropy (ΔS), enthalpy (ΔH), and Gibb's free energy (ΔG) as variables at different temperatures. The maximum activation energy, rate constant, enthalpy and entropy are 59.31 kJ mol−1, 27.15 s−1, 65.87kJmol-1 and 27.63kJmol-1k−1 respectively. The important parameters affecting the process of biodiesel production is reaction temperature and catalyst concentration. Here, the nano magnetic catalysts accelerate the production of biodiesel from fish waste. So, the biodiesel yield is high at 55 °C reaction temperature (95 wt%) than 32 °C reaction temperature (65 wt%). The resulting biodiesel was tested according to ASTM standards and obtained the closer values as per ASTM D6751 specification.