AbstractBiodiesel from vegetable oils has attracted a great deal of interest as a alternative fuel for fossil diesel. This effort able to reduce the dependence on petroleum based fuels and provides a fuel with more benign environmental. Non-edible vegetable oils are attracting more attention than edible oils due to the concern on food versus fuel and other environmental issues. Cerbera manghas oil is one of the promising non-edible feedstocks. Biodiesel production from cerbera manghas vegetable was first time reported and investigated. The viscosity of crude oil was 32.83 mm2/s and 12.64mg KOH/g for acid value which is far above the 2%. Therefore, cerbera manghas methyl ester (CMME) was produced via two step acid-alkaline transesterification using H2SO4 as acid catalyst and KOH as alkaline catalysts. Hence 98.5% of biodiesel was achieved with 9:1 methanol in presence of 1% sodium hydroxide. Moreover, blending of CCME with diesel resulted in an improvement which the viscosity is reduced to 3.54 mm2/s. On the other hand, the blending of CMME also shown the remarkable improvement in oxidation stability (14.55hours). The properties of cerbera manghas methyl ester fell within the recommended biodiesel standards. It can be conclude that cerbera manghas oil is promising feedstock for future production of biodiesel