AbstractBanana peel and biodiesel derived glycerol are one of the most abundant agricultural waste in Southeast Asian countries. This current research demonstrated potential biomethane production from co-digestion of agricultural wastes under mesophilic fermentation. The wastes used include (1) banana peel (varying total solids in the range 2.5-10% w/v) and (2) Banana peel mixed with pure or waste glycerol. All treatments were setup in duplicate in 0.5 L batch reacotr. Gas and liquid samples were collected every 12 and 24 hr, respectively. Maximum methane production potential and yield were 181 mL and 188mL g-1 TS at 7.5% banana peel. The maximum methane production potential and yield of 7.5% banana peel mixed with 7.5 g L-1 pure glycerol (in the presence of buffer) were 467 mL and 151 mL g-1 COD, respectively, while those of 7.5% banana peel mixed with 7.5 g L-1 waste glycerol (in the presence of buffer) were 652 mL and 281 mL g-1 COD, respectively. Small amount of VFAs and ethanol was accumulated in the reactor content. The current findings indicate the potential use of glycerol waste as a co-substrate in biomethane production.