AbstractSoybean is a prospective source of biofuel in Indonesia due to adaptive in Indonesian agro ecology, which is 75 d to 85 d to maturity, and high oil seed content. A total of twelve soybean genotypes tested in eight locations in Indonesia in 2013 to assess its potential yield and seed oil production. Seed yield stability was assessed by (Additive Main Effect and Multiplicative Interaction) AMMI method. The seed yield range was 2.15 t ha–1 to 2.79 t ha–1 with an average of 2.54 t ha–1. Seed oil content ranges from 16.57% to 18.85% with an average 17.6%. Four soybean (G5, G7, G9, and G10) as stable genotypes. G9 (L.Jateng×Sinabung-85) prospective to be used for biodiesel development in Indonesia due to its high yield (2.67 t ha–1) and the oil content of 18.85%, hence its seed oil production reach 504kg ha–1. G9 have 80 d to maturity. Potential production of soybean seed oil is determined by the seed yield and oil content. G9 could potentially be used for biodiesel development in Indonesia.