Abstract We herein report the extraction of biodiesel (S1B and S2B) from a two dissimilar Scenedesmus species namely Scenedesmus obliquus (S1) and Scenedesmus dimorphus(S2) by means of transesterification reaction of lipid moieties, using absolute alcohol and Ni/ H2 as medium with the presence of Ni(II)–Schiff base compound as promoter to yield biodiesel. The feature of extracted micro algal lipids was investigated via ultimate, analytical along with spectral studies. The engine characteristics with the biodiesel-diesel blends (S1B10, S1B20 &S1B50 and S2B10, S2B20 &S2B50) demonstrate that there is a slight increase in specific fuel consumption with low brake power. Additionally, decrease in the emissions of GHGs was observed with all the diesel blends, except for NOx. In addition, the blends show distinguished in vitro antimicrobial and antioxidant activities. The thermal behavior and corrosion inhibition of the biodiesel samples were analyzed.