Abstract Side by side biomass productivities, harvesting time (autumn vs. winter) and frequency (annual vs. biennial) of three perennial grasses were compared under northern and southern Mediterranean climates. Miscanthus ( Miscanthus × giganteus Greef et Deu.) was compared to giant reed ( Arundo donax L.) in Catania (37°24′N, 15°03′E), and to switchgrass ( Panicum virgatum L.) in Bologna (44°55'N, 11°45′E). Generally, giant reed produced about 50% higher biomass than miscanthus in Catania. Miscanthus almost halved biomass productivity in the driest year, while giant reed reduced biomass yield by only 30% under the same conditions. In Bologna, miscanthus and switchgrass produced similar amounts of cumulative biomass over 6 years. Switchgrass kept more stable annual yields than miscanthus, which, however, evidenced a higher potential under favorable climate conditions. Autumn harvest significantly reduced biomass productivity and quality (moisture content, ashes, cellulose and hemicellulose), particularly under South Mediterranean climate. In Catania, autumn biomass was 50% (giant reed) to 85% (miscanthus) lower than winter biomass, while in Bologna, autumn cut reduced switchgrass yield by 20%. Biennial harvesting resulted in almost 40% lower cumulative biomass yield than annual cut, after 6 years.