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Validation of an HIV-1 inactivation protocol that is compatible with intracellular drug analysis by mass spectrometry

descriptionPublicationkeyboard_double_arrow_right Article , Journal 01 Feb 2007 Netherlands Publisher:Elsevier BVJournal:Journal of Chromatography B, volume 847, pages 38-44 (issn: 1570-0232,

Authors: Albert D. M. E. Osterhaus; Peter C. Burgers; Theo M. Luider; Ronald de Groot; Esther J. Verschuren; Rob A. Gruters; Jeroen J. A. van Kampen;

doi: 10.1016/j.jchromb.2006.10.001

pmid: 17055786

Validation of an HIV-1 inactivation protocol that is compatible with intracellular drug analysis by mass spectrometry

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Abstract

Mass spectrometry is a powerful tool for studying the intracellular pharmacokinetics of antiretroviral drugs. However, the biohazard of HIV-1 calls for a safety protocol for such analyses. To this end, we extracted HIV-1 producing cells with methanol or ethanol at 4 degrees C. After extraction, no viral infectivity was detected, as shown by a reduction in infectious titers of more than 6log. In addition, this protocol is compatible with the quantitative analysis of antiretroviral drugs in cell extracts using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. Thus, using this protocol, infectious HIV-1 is inactivated and antiretroviral drugs are extracted from cells in a single step.

Country

Netherlands

Related Organizations

Erasmus University Medical Center
Netherlands
Erasmus University Rotterdam
Netherlands

Keywords

Cell Extracts, Ritonavir, Ethanol, Anti-HIV Agents, Methanol, Sensitivity and Specificity, EMC MM-03-44-06, EMC MM-04-27-01, SDG 3 - Good Health and Well-being, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, HIV-1, Humans, Virus Inactivation, Cells, Cultured

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