Abstract: Mountain areas have been the testbed for sustainable development models focused on balancing their vulner-ability and the value of their natural, cultural, and social resources. In these areas, the continuous adaptative interaction between bio-geophysical and socio-cultural processes assembles Socio-Ecological Systems (SES) characterized by a great diversity of ecosystems and land uses, which provide substantial support for the live-lihoods of mountain communities and essential ecosystem services for uplands and lowlands. In this paper, we take value chains to be the operative units of analysis to examine human-natural systems interactions in mountain areas. Value chains mobilize resources and connect actors beyond territorial boundaries and economic sectors to generate economic, environmental, and social values. Strategies for local development of these areas should direct attention to value generation activities and require a systemic, integrated and assets-based approach which explores the potential synergies emerging from the coordination of the diversity of local spec-ificities and considers the opportunities and threats emerging from external sub-systems. This paper proposes a novel comparative framework to characterize value chains contribution to resilient and sustainable development of SESs in mountain areas. This framework is meant for researchers and policy analysts to identify the role value chains might accomplish for a better balance between natural resource conservation and sustainable socio-economic development in European (remote) rural areas. Two mountain food value chains are used to illus-trate the framework and test its efficacy. The cases depict two value chain configurations which result in different social, economic and environmental outcomes for the sustainable development of the SES.