In India the demand of sustainable energy is growing at a fragmented level. The demand of rural electrification and economic growth requires high production of energy. Hence, the use of renewable energy may help to mitigate air pollution, and allow specific regenerative agriculture, waste, and forest biomass for producing the energy and achieving sustainable development goals. In the current work, we have selected a district of Maharashtra state of India as a case study and mapped the different land cover categories, and their estimated biomass. Based on this we have prepared the biomass thematic maps for the study area. These biomass maps have been prepared in the GIS environment and they displayed the biomass of crops, forests, and wastelands. The studied district has huge potential for agricultural biomass and can act as biofuel and biomass economies. These maps may play a crucial role in upgrading biomass sites focused on generating power from plants in the district Akola of Maharashtra, India.