Abstract In this study, biodiesel was produced from safflower seeds by converting the raw oil obtained through dampening them via the rolling process, roasting at 90 °C and pressing into Safflower Oil Methyl Esther (Safflower Biodiesel) using the trans-esterification method. Experimental fuels were obtained in the forms of D100, B2.5M2.5D95, B5M5D90, B5M2.5D92.5 and B2.5M5D92.5 by mixing the biodiesel fuel obtained from safflower with diesel fuel, adding bio-ethanol at the rates of 2.5% and 5%, and volumetrically in inverse ratio. Tests were conducted to determine the fuel properties of the mixed fuels obtained and the diesel fuel, their kinematic viscosity, density, water content, pH level, caloric value, flash point, clouding, pour and freezing points, copper bar corrosion test, iodine number, CFPP (Cold Filter Plugging Point) test and cetane number. Moreover, the mixtures obtained and the diesel fuel were tried and examined in a water-cooled, four-stroke, single cylinder diesel engine that had a direct injection fuel system. As a result of the experiments, performance characteristics of the test engine were obtained. Exhaust emission values (CO, CO2, HC, O2, SO2, NOx) were obtained from the exhaust stack of the test engine using the probe of the gas analysis device.