Abstract Two thirds of Canadians reside in urban areas and 85% of recent population growth occurs in these areas. The intensity and duration of extreme hot weather events are predicted to increase in Canadian cities and in cities globally. It is well established that human suffering due to extreme heat is exacerbated in urban as compared to rural environments. Understanding the characteristics of urban landscapes that play the greatest roles in exacerbating the human health impact of extreme heat is thus imperative. This study explores the relationship between the amount of canopy cover from trees and the incidence of heat-related morbidity during extreme heat events in 544 neighbourhoods of Toronto, Ontario, Canada. Four extreme heat events from three years were studied. Heat-related ambulance calls were found to be 12.3% higher during the heat events than in the preceding or the following week. The number of heat-related ambulance calls was negatively correlated to canopy cover (Spearman Rank rho = −0.094, p = 0.029) and positively correlated to hard surface cover (Spearman Rank rho = 0.150, p 5% could reduce heat-related ambulance calls by approximately 80%. These results have important implications for human health during heat events, particularly in the context of global climate change and urban heat islands, both of which are trending toward hotter urban environments in future.